LOCUS       sequence-181823-       18875 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate 
            peroxidase (APX) in plant cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18875)
  AUTHORS   Exposito-Rodriguez M, Laissue PP, Yvon-Durocher G, Smirnoff N, 
            Mullineaux PM
  TITLE     Photosynthesis-dependent H2O2 transfer from chloroplasts to nuclei 
            provides a high-light signalling mechanism.
  JOURNAL   Nat Commun. 2017 Jun 29;8(1):49. doi: 10.1038/s41467-017-00074-w.
  PUBMED    28663550
REFERENCE   2  (bases 1 to 18875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-06-29"; volume: "8(1):49. doi"; pages: " 
            10.1038/s41467-017-00074-w"
COMMENT     Sequence Label: pGem-nHyPer:cAPX
FEATURES             Location/Qualifiers
     source          1..18875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(145..516)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            549..658
                     /note="incP origin of transfer"
     primer_bind     881..900
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1134..1151
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1339..1989)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2454..2478
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2602..2781
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2634..2817
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2838..3632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2892..2911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3502..3521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4022..4274
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4823..4844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4859..4864,4865..4882,4883..4889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4897..4913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4902..4924
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4921..4937
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4921..4937
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      complement(4968..5220)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    5295..5319
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6109..6133)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7244..7589
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    7611..7635
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7659..7679
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9111..9131
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9151..9175)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9207..9459
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(9888..10140)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10608..11633)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(10608..11633)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(11643..11987)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(12105..12122)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12105..12121)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12114..12136)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12261..12280)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12471..12492
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    12739..12763
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14359..15507)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(15806..16600)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  16792..17559
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      join(18252..18875,1..8)
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tgagcgtcgg aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttcctag gatccccgat ctagtaacat
     4981 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     5041 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     5101 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     5161 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     5221 gggaattgga tccggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat
     5281 tcgcccttgg atccaccact ttgtacaaga aagctgggtc ggcgcgccca cccttttaag
     5341 catcagcaaa cccaagctca gaaagcttca tgtgggcctc agcgtaatca gcgaaaaagg
     5401 catcttcatc agcagcgtat ttctcgacca aaggacggaa aacagggtcg tccaatagtg
     5461 ctttgtcaga gacaagctga agaaggcctt ccttctctcc gctcaagagt tccttgaagt
     5521 aagagttgtc gaagattaga gggtttgatg tccatgcacc ttcgaagcca gacctatcct
     5581 tgtggcatcg tcccagagtg tgggcaccag ataaagcgac aatgtctttg tcagataagc
     5641 ccatctgctt agcaaagaca tctctcaaat ggtcacaacc cttggtagca tcaggaagac
     5701 gaccctctgg aggtggttgg ggcttgtcct ctcttccagg gtggaaagga atgtcagggc
     5761 caccagtaac ttcaacggcc acaacaccag caagctgatg gaaatcagca aaagagatgg
     5821 tagggaattg ctccctgatg gggtccaaca acctaagagc aatgtggata ccactgttgg
     5881 ctccatgagc ttgctcagcg tcaaacctca ttgttccgaa tggacctcca gtccttgatt
     5941 gacaatcgaa agttccagca gagtgccatg cgagtcggac catgatgggt gcacagttct
     6001 tctcagcgat caaacctctg agcttcctcc tgcacttctc aacagccttc ttgtaatctt
     6061 cgctcacggt tgggtagttc ttcgtcatgg tgaagggggc ggccgcggag cctgcttttt
     6121 tgtacaaact tgttctagaa ggattggttg agtatctgat gatccttcaa atgggaatga
     6181 atgccttctt atatagaggg aattcttttg tggtcgtcac tgcgttcgtc atacgcatta
     6241 gtgagtgggc tgtcaggaca gctcttttcc acgttatttt gttccccact tgtactagag
     6301 gaatctgctt tatctttgca ataaaggcaa agatgctttt ggtaggtgcg cctaacaatt
     6361 ctgcaccatt ccttttttgt ctggtcccca caagccagct gctcgatgtt gacaagatta
     6421 ctttcaaaga tgcccactaa ctttaagtct tcggtggatg tctttttctg aaacttactg
     6481 accatgatgc atgtgctgga acagtagttt actttgattg aagattcttc attgatctcc
     6541 tgtagctttt ggctaatggt ttggagactc tgtaccctga ccttgttgag gctttggact
     6601 gagaattctt ccttacaaac ctttgaggat gggagttcct tcttggtttt ggcgatacca
     6661 atttgaataa agtgatatgg ctcgtacctt gttgattgaa cccaatctgg aatgctgcta
     6721 aatcctgagc tcaagcttgc atgcctgcag gtccccagat tagccttttc aatttcagaa
     6781 agaatgctaa cccacagatg gttagagagg cttacgcagc aggtctcatc aagacgatct
     6841 acccgagcaa taatctccag gaaatcaaat accttcccaa gaaggttaaa gatgcagtca
     6901 aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc aagatcagaa
     6961 gtactattcc agtatggacg attcaaggct tgcttcacaa accaaggcaa gtaatagaga
     7021 ttggagtctc taaaaaggta gttcccactg aatcaaaggc catggagtca aagattcaaa
     7081 tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag agtctcttac
     7141 gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca cttgtctact
     7201 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa
     7261 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga
     7321 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca
     7381 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
     7441 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
     7501 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     7561 aaggaagttc atttcatttg gagagaacac gggggactct agagttatca acaagtttgt
     7621 acaaaaaagc aggctccgcg gccgccccct tcaccatgcc taagaagaag agaaaggttg
     7681 aaatggcttc tcaacagggt gagactatgt caggaccatt gcatatcggt ctaatcccaa
     7741 cagttggacc ttacttactt cctcacatta tcccaatgct acaccaaact ttccctaaac
     7801 ttgaaatgta ccttcacgag gcacagactc atcaactatt ggcccagtta gatagtggta
     7861 aacttgattg tgtcatacta gctttggtaa aggaatccga ggcattcata gaagtgcctc
     7921 ttttcgatga acctatgctt ttagcaatct atgaagatca cccatgggcc aatagagaat
     7981 gcgttccaat ggcagatttg gccggtgaga aacttctaat gcttgaggat ggacactgct
     8041 tgagggatca agctatgtcc gccggttaca attcagataa cgtctatatc atggccgaca
     8101 aacagaagaa tggtataaag gccaacttca aaattagaca taacgtggaa gatggttccg
     8161 tacaactagc agaccattat caacagaaca ccccaatcgg tgacggacct gttttacttc
     8221 cagataacca ctacctatct tttcagtctg tcctatccaa agacccaaat gagaaaaggg
     8281 atcacatggt gttgcttgag ttcgtaaccg ctgccggtat tactcttggt atggatgagc
     8341 tatacaatgt agacggaggt agtggaggta caggttccaa gggagaagag ttgttcacag
     8401 gagttgtccc aatattagta gaacttgatg gtgacgtgaa cggtcacaaa ttttcagtct
     8461 ccggagaagg tgagggtgac gcaacctacg gaaaactaac attgaaatta atctgtacaa
     8521 ctggaaaact tcctgtccca tggccaacat tagtaacaac cttaggttac ggtttaaagt
     8581 gcttcgcaag atatcctgac catatgaagc aacatgactt cttcaagagt gctatgcctg
     8641 agggatatgt gcaagagagg actatattct tcaaagacga cggtaattat aaaactaggg
     8701 cagaagtgaa gtttgaggga gatactttgg tgaatagaat agagcttaaa ggtatcggat
     8761 tcaaagagga cggaaatatt cttggacaca agctagagta caacggaacc ggattttgct
     8821 tcgaagccgg tgctgacgag gatactcact tcagggctac atcactagaa accttgagga
     8881 acatggtagc cgttggaagt ggaataactt tgttaccagc acttgccgtg ccaccagaga
     8941 ggaagaggga tggtgtagtt tatcttccat gcattaaacc agagccaaga aggaccattg
     9001 gattggtcta caggccaggt tcaccattga gatcaaggta tgaacagtta gcagaggcta
     9061 taagagccag gatggatggt cactttgata aggttttgaa gcaagcagtg cctaagaaga
     9121 agagaaaggt ttgaaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata
     9181 acagcgctta gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     9241 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     9301 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     9361 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     9421 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattagct tcatcaacgc
     9481 aagacatgcg cacgaccgtc tgacaggaga ggaatttccg acgagcacag aaaggacttg
     9541 ctcttggacg taggcctatt tctcaggcac atgtatcaag tgttcggacg tgggttttcg
     9601 atggtgtatc agccgccgcc aactgggaga tgaggaggct ttcttggggg gcagtcagca
     9661 gttcatttca caagacagag gaacttgtaa ggagatgcac tgatttatct tggcgcaaac
     9721 cagcaggacg aattagtggg aatagcccgc gaatatctaa gttatgcctg tcggcatgag
     9781 cagaaacttc caattcgaaa cagtttggag aggttgtttt tgggcatacc ttttgttagt
     9841 cagcctctcg attgctcatc gtcattacac agtaccgaag tttgatcgat ctagtaacat
     9901 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     9961 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
    10021 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
    10081 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
    10141 tgcttcgacg cactccttct ttactccacc atctcgtcct tattgaaaac gtgggtagca
    10201 ccaaaacgaa tcaagtcgct ggaactgaag ttaccaatca cgctggatga tttgccagtt
    10261 ggattaatct tgcctttccc cgcatgaata atattgatga atgcatgcgt gaggggtatt
    10321 tcgattttgg caatagctgc aattgccgcg acatcctcca acgagcataa ttcttcagaa
    10381 aaatagcgat gttccatgtt gtcagggcat gcatgatgca cgttatgagg tgacggtgct
    10441 aggcagtatt ccctcaaagt ttcatagtca gtatcatatt catcattgca ttcctgcaag
    10501 agagaattga gacgcaatcc acacgctgcg gcaaccttcc ggcgttcgtg gtctatttgc
    10561 tcttggacgt tgcaaacgta agtgttggat cccggtcggc atctactcta ttcctttgcc
    10621 ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg
    10681 gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat
    10741 cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc
    10801 aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat
    10861 cctgcaagct ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac
    10921 cacggcctcc agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg
    10981 ctccagtcaa tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa
    11041 tccgcgtgca cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg
    11101 agagcctgcg cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca
    11161 tggggatcag caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc
    11221 ggtccgaatg ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg
    11281 gcctccgcga ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg
    11341 acaccctgtg cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca
    11401 agcacttccg gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg
    11461 tagaaaccat cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag
    11521 ctgaaagcac gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac
    11581 ttttcgatca gaaacttctc gacagacgtc gcggtgagtt caggcttttt catatcgggg
    11641 tcgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
    11701 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
    11761 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
    11821 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
    11881 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
    11941 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
    12001 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
    12061 tgacggatct ctaggacgcg tcgacgcgtc ctagaagcta attcactggc cgtcgtttta
    12121 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    12181 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    12241 cgcagcctga atggcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
    12301 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
    12361 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
    12421 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
    12481 gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat
    12541 tttgccgatt tcggaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
    12601 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
    12661 tcactggtga aaagaaaaac caccccagta cattaaaaac gtccgcaatg tgttattaag
    12721 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
    12781 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
    12841 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
    12901 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
    12961 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aaatatcatc tccctcgcag
    13021 agatccgaat tatcagcctt cttattcatt tctcgcttaa ccgtgacagg ctgtcgatct
    13081 tgagaactat gccgacataa taggaaatcg ctggataaag ccgctgagga agctgagtgg
    13141 cgctatttct ttagaagtga acgttgacga tatcaactcc cctatccatt gctcaccgaa
    13201 tggtacaggt cggggacccg aagttccgac tgtcggcctg atgcatcccc ggctgatcga
    13261 ccccagatct ggggctgaga aagcccagta aggaaacaac tgtaggttcg agtcgcgaga
    13321 tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc cacgccaggc
    13381 cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa gctactggaa
    13441 cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga ggcacgggag
    13501 gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc gccaggcccg
    13561 ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc gccacgcccg
    13621 cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct agcagagcgg
    13681 cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg accccgcccg
    13741 gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta agtgcgccga
    13801 ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc atcacgagca
    13861 ataaacccgc cggcaacgcc cgcagcagca taccggcgac ccctcggcct cgctgttcgg
    13921 gctccacgaa aacgccggac agatgcgcct tgtgagcgtc cttggggccg tcctcctgtt
    13981 tgaagaccga cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc acggcatctc
    14041 gcaaccgttc agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa acggacccga
    14101 acatctctgg agctttcttc agggccgaca atcggatctc gcggaaatcc tgcacgtcgg
    14161 ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt caattttaat cctctgttta
    14221 tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc aagtgcgtcg
    14281 agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac ccccagccgg
    14341 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
    14401 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
    14461 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
    14521 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
    14581 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
    14641 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
    14701 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
    14761 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
    14821 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
    14881 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
    14941 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
    15001 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
    15061 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
    15121 ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag
    15181 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
    15241 cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca cggcggccga
    15301 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
    15361 ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
    15421 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
    15481 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
    15541 cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc
    15601 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
    15661 attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc
    15721 ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc
    15781 ggcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata taatatttta
    15841 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    15901 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    15961 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    16021 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    16081 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
    16141 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    16201 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    16261 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    16321 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
    16381 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    16441 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    16501 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    16561 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    16621 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    16681 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    16741 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat gggtgatgct
    16801 gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg cttctgtttc
    16861 tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca aaagcaccgc
    16921 cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc agttcactta
    16981 caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga atggcgttgg
    17041 atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac gtcacttaaa
    17101 aaactcaggc cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt catcgtctgc
    17161 gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg ctggctgttt
    17221 tacgcgtatg acagtctccg gaagacggtt gttgcgcacg tattcggtga acgcactatg
    17281 gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat atggatgacg
    17341 gatggctggc cgctgtatga atcccgcctg aagggaaagc tgcacgtaat cagcaagcga
    17401 tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc acggctggga
    17461 cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat cgggcattat
    17521 ctgaacataa aacactatca ataagttgga gtcattaccc aattatgata gaatttacaa
    17581 gctataaggt tattgtcctg ggtttcaagc attagtccat gcaagttttt atgctttgcc
    17641 cattctatag atatattgat aagcgcgctg cctatgcctt gccccctgaa atccttacat
    17701 acggcgatat cttctatata aaagatatat tatcttatca gtattgtcaa tatattcaag
    17761 gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg tagcttttta aatatggcgc
    17821 ttcatagagt aattctgtaa aggtccaatt ctcgttttca tacctcggta taatcttacc
    17881 tatcacctca aatggttcgc tgggtttatc gcacccccga acacgagcac ggcacccgcg
    17941 accactatgc caagaatgcc caaggtaaaa attgccggcc ccgccatgaa gtccgtgaat
    18001 gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc actgcccggc
    18061 acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc gggcgtcgcg
    18121 ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg caatggcaag gactgccagc
    18181 gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg gggggatggg
    18241 aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct tcggcgtgcg
    18301 cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata ttggtttaaa
    18361 agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct tgcaaatgct
    18421 ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc atctgtcagc
    18481 actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg cgcccctcaa
    18541 gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg ggaaactcgc
    18601 gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg ccggccgaaa
    18661 tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc aagtgtcaac
    18721 gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac aacgccggcg
    18781 gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag ggccatagac
    18841 ggccgccagc ccagcggcga gggcaaccag cccgg
//
