LOCUS       sequence-181826-        8288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression of nuclear green-to-red photoconvertible 
            Dendra2 and the membrane blue fluorescent protein Cerulean..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Dempsey WP, Fraser SE, Pantazis P
  TITLE     PhOTO zebrafish: a transgenic resource for in vivo lineage tracing 
            during development and regeneration.
  JOURNAL   PLoS One. 2012;7(3):e32888. doi: 10.1371/journal.pone.0032888. Epub 
            2012 Mar 14.
  PUBMED    22431986
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e32888. doi"; pages: " 
            10.1371/journal.pone.0032888. Epub 2012 Mar 14"
COMMENT     Sequence Label: PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
FEATURES             Location/Qualifiers
     source          1..8288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     180..199
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     439..460
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      502..511
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      565..574
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(664..682)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(665..682)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(3397..3584,3585..3596)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(3630..3646)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3630..3646)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3683..3703)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3683..3701)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3722..3738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3722..3738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3735..3757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3746..3762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3770..3776,3777..3794,3795..3800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3815..3836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3953..3970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4124..4712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4204..4223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4883..5674,5675..5743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5506..5525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5744..5848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6011..6032)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6223..6242
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6386..6408
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6400..6417
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6401..6417
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6427..6446
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6427..6445
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(6476..6487,6488..6625)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     polyA_signal    6717..6851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6754..6773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6808..6827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6858..6876
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(6910..7599)
                     /codon_start=1
                     /product="monomeric photoswitchable green-to-red 
                     fluorescent protein from Dendronephthya"
                     /label=Dendra2
                     /note="mammalian codon-optimized"
                     /translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
                     NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
                     ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
                     MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
                     SPLPSQVW"
     CDS             complement(7618..7995)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             complement(8002..8055)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(8077..8288,1..499,500..502,503..505))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8119..8140)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatcttgaa gttggccttg atgccgttct tctgcttgtc ggcggtgata tagacgttgt
       61 cgctgatggc gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
      121 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
      181 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
      241 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcgggcg aagcactgca
      301 cgccccaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
      361 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
      421 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
      481 acagctcctc gcccttgctc accatggtgg cgaccggtgc ctcgtcgtcg ttcaggttgt
      541 ccttgcgctt gctcttgatg cagcccatgg cggcctcgtc gtcgttcagg ttgtccttgc
      601 gcttgctctt gatgcagccc atatcgatgg gatcctgcaa aaagaacaag tagcttgtac
      661 tcttctatag tgtcacctaa ataggcctag gatggcgaac tgtaaaagaa agggaaactg
      721 ttagaattgc actgctgcca gggagaggcg tttcagcaag catgtgacca tctggagtca
      781 acttcctgtt ttgctctgca ttctacagtc tgaccgctgg tgggcgctag tgacagcgcg
      841 cgcctcagat atgccagaca aaggaagtcc ctctgcattc tctcattatt accataaaag
      901 gcaatggttt gagccgcttt gcggctgccg gcggtgtggc actcagccag agtcgtaggg
      961 ctcagtaaaa aggaacagat ttcgacattc atcacaccag gaccactccc taaattttac
     1021 acaaatgaag tttcacatcc tcttccaccc tcgcctgcga tcaattacac aaccgcattt
     1081 caacgggtgt ctgccgccgg gtgttactgc gtcacagatc tgcagtcagt ccacggactc
     1141 ctcgtgagcg gagacttcac actaaagcat ttctggacgg tcactctcag tcggttcagt
     1201 tcatagtcta gcctcaaaaa tcaccgagat cctgaaaaac tgatgggctc tgcttcgaca
     1261 gtcacagcgg ctgaagtgca gccagagaac tccgcagccg ttattaaagt ggaccacacc
     1321 tctaaaattc aaatctttca tagcggatac aacacttcac taacgttaac gttaatcttc
     1381 agcttcatta aagacagagg gggctgcagg ctacatttaa atagaacaca taacaaaaag
     1441 aaaattaacc aagttaaaac taaatgccaa tacccgttac taataacaag atcttcgatc
     1501 ttataaagca cgaatgagcc gatgttagat tgatggggtt tacgttacat cagatgagag
     1561 ctggttagcc ggctagctaa agtaggtcac accctgctgc agcactgcct gcacaaagaa
     1621 cttcccatgc cggctatcca tcaagtgtgc aacatgctca ccagcaccct caaaactgta
     1681 gttaaaaatc acatttatgg ctgttaaaga gagattagcc agaacacaac tttatattaa
     1741 gacttaacac ccatttcaca ccggcctatt caatgttcaa tgtcgtgcct acgactggtc
     1801 acgttaaatt accgacattc tcacctgcca catccactaa cgctacaaaa aagttaagat
     1861 acagccaata tttattaaat ataacataga ttatcaaaag aaccaccact tataaatcaa
     1921 acctagtaga taactacaaa gagaccgtta tgaactgtct aaagacttca tcaaatcata
     1981 tttatccatt tacacatttt acttaacatt aatagataga aactataatt aaacgcttaa
     2041 aaactgtaaa accaactcac cttgggtttg gggggttgtc gacttaagag aatgggttac
     2101 aagcagatga tattccgcac tagctgcgtg tggaggagct caaagtgagg ctgaaaagct
     2161 gggcgcgctc ttttatatga cggcacatac ccggctctag ccataaaagg taaactttcg
     2221 gagctctgtg ctctgattgg tccagcgtca cacacccggc tgtcgcgtgc acattgctac
     2281 acttgcttct tgcaacttga gagtgaaaga aggggaagct taactggtta aagaacaagg
     2341 ccatggcgat ttcctataaa actggaattt ttatttgcaa attttaacac agaatttttc
     2401 gtatttttct gcgacaacgc caagactcaa ttaaactttt caaaaagtct gtgcagagtt
     2461 acaagtggca gaatgcgtca taatgatgga tgcctccatg atcctgtgtt gtgttttcaa
     2521 tctaacacaa cagcagctct gtaggtcatt tacctcttgt gttcacgaaa taccgtaata
     2581 caaacaatgg gggatctaat gagtactttt ccatgtataa aatggacatt ttttttatcc
     2641 aaattaggat gacactactt gtagtatgtt gtatgagcaa ctagctggtc ttagctcagt
     2701 agtaatgcac tttaagaaag tgctttaata actttgaatg aatgggacct gcattccatg
     2761 ccacattgct tcactatttt tgtgtgtaca agttacacaa tgatgtataa agtgagtgat
     2821 agcagttaca tttttgcaag tgggcctacc tcaggtttaa tgttcattag aaatgttata
     2881 tgtcaagtct tgatcttaaa tagttaaatt ttctgttcat aatttcatgt cgtgtctatg
     2941 cagacataac atttcttttt aaagaggttt ataaggataa atcctaagaa cataattctc
     3001 attaaaagta gaaaaaaaaa aacactgaaa gtgttggtga tcagctacaa taaataatat
     3061 tttgcacaca tttcagatat tgccacactg aataacagat aaagctgtgc ttgagcagca
     3121 gtgatcatga taaactacat gtgacttttt actgtgtaac ttgggagtaa tgcacatggc
     3181 cttcagtggg agttataaac aaaaacagct gattgtgtta aaagtgcttg ctgataccaa
     3241 ggacaatgag gtgatgtgac ttttattttg tttttggcaa attccaagta ttttgacaag
     3301 actttttaca attctgagca ttttacctct aaaatatgga agtttctcag agtaaacgtt
     3361 gagtataggt aatgactagg ccctcgagcc gggcccaagt gatctccaaa aaataagtac
     3421 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     3481 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     3541 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
     3601 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
     3661 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     3721 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3781 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3841 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3901 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3961 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4021 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4081 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4141 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     4201 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     4261 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     4321 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     4381 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     4441 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     4501 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4561 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4621 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4681 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4741 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4801 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4861 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4921 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4981 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5041 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5101 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5161 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5221 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5281 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5341 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5401 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5461 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5521 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5581 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5641 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5701 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5821 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     5881 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     5941 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     6001 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     6061 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     6121 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     6181 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     6241 agcgggcgct agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     6301 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     6361 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     6421 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg gcatatggtt cttgacagag
     6481 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     6541 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     6601 tccattaaaa ttgtacttga gtattagatc tgcatgcgtt taaacccggg atatcaaaaa
     6661 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     6721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6841 atgtctggat caccggtgct agttattgct cagcgggacg tctcacttgt acacgccgct
     6901 gtcgccggac cacacctggc tgggcagggg gctgtagcgg gccacggcgt gctcgtacag
     6961 cttcaccttg ttgtagtcgc tgtcgttgcc caggatctcg atgcggtggt ccacgaagtg
     7021 ggcgtcgggc agctgcacca ccttcttggc cttgtaggtg gtcttgaagt cgcacaggta
     7081 gtggccgccg ccctccagca gcagggccat gttgatgttg cccaccagca ggccgtcgcg
     7141 cacgtgcagc ttctcggtgc tgggctccca cttcagggtc ttcttctgca tcacggggcc
     7201 gttggggggg aagttggtgc ccttgaagcg cacgttctgg aagaagcagt cgccctccag
     7261 gctgatgtcg ctgcggatgg tgcagatgcc cttgtcctcg aaggtcatgg tgcgctccca
     7321 gctgtagccc tcggggaagc tctgcttgaa gtagtcgggg atgtcctcgg ggtacttggt
     7381 gaacacccgg ttgccgtagt gcacggcggt ggtcaggatg tcgtagctga agggcagggg
     7441 ggcgccctcc ttcacggtca ggttggcggt ctgggtgccc tcgtagggct tgcccttgcc
     7501 ctcgccctcg atcacgaagg cgtggccgtt cacgttgccc tccatgtgca ccttcacgcg
     7561 catgtcctcc ttgatcaggt taattcccgg ggtgttcatg gtggcgaccg gtggatcctt
     7621 agcgctggtg tacttggtga tggccttagt accctcggac acggcgtgct tggccaactc
     7681 cccaggcagc agcaggcgca cggccgtctg gatctccctg gaggtgatgg tcgagcgctt
     7741 gttgtaatgc gccaggcggg aagcctcacc tgcgatgcgc tcgaaaatgt cgttcacaaa
     7801 cgaattcatg atgcccatgg ccttggacga aatgccggtg tcagggtgga cctgcttcag
     7861 aaccttgtac acatagatgg aatagctctc cttgcggctg cgcttgcgct tcttgccgcc
     7921 tttcttctgc gccttagtca ccgccttctt ggagcccttt ttcggggcgg gagcagactt
     7981 cgctggctct ggcattacgt aagggccggg attctcctcc acgtcaccgc atgttagaag
     8041 acttcctctg ccctctcctg atcccttaat taaactcttg tacagctcgt ccatgccgag
     8101 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     8161 gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     8221 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     8281 gttgtggc
//
