LOCUS       sequence-181827-        5014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: GFP, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3010
FEATURES             Location/Qualifiers
     source          1..5014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2908..2936)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3531..3551
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      3602..3850
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3922..4026
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgcctg cagtgcagcg tgacccggtc
      901 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
      961 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
     1021 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
     1081 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
     1141 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
     1201 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
     1261 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
     1321 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
     1381 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
     1441 ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag
     1501 cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc
     1561 ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca
     1621 gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct
     1681 cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg
     1741 cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg
     1801 agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag
     1861 gtacgccgct cgtcctcccc ccccccctct ctaccttctc tagatcggcg ttccggtcca
     1921 tggttagggc ccggtagttc tacttctgtt catgtttgtg ttagatccgt gtttgtgtta
     1981 gatccgtgct gctagcgttc gtacacggat gcgacctgta cgtcagacac gttctgattg
     2041 ctaacttgcc agtgtttctc tttggggaat cctgggatgg ctctagccgt tccgcagacg
     2101 ggatcgattt catgattttt tttgtttcgt tgcatagggt ttggtttgcc cttttccttt
     2161 atttcaatat atgccgtgca cttgtttgtc gggtcatctt ttcatgcttt tttttgtctt
     2221 ggttgtgatg atgtggtctg gttgggcggt cgttctagat cggagtagaa ttaattctgt
     2281 ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca tacatattca
     2341 tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat acatgttgat
     2401 gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt gatgatgtgg
     2461 tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt caaactacct
     2521 ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata gttacgagtt
     2581 taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg ttttactgat
     2641 gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga gtacctatct
     2701 attataataa acaagtatgt tttataatta ttttgatctt gatatacttg gatgatggca
     2761 tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt tatttgcttg
     2821 gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttcctg caggatggtg
     2881 agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
     2941 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     3001 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aaccctggtc
     3061 accaccctga cctacggcgt gcagtgcttc tcccgttacc ctgatcatat gaagcggcac
     3121 gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag
     3181 gacgacggga actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac
     3241 aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg
     3301 gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc
     3361 aaagccaact tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat
     3421 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
     3481 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     3541 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataactcgag
     3601 atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt
     3661 gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat
     3721 gtaagatcac attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt
     3781 ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat
     3841 taaagataag gagctccggt gacggacgtg ggcaggtgat gaaaagcctt agggtcaggt
     3901 ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt tatttttcta aatacattca
     3961 aatatgtatc cgctaatgag acaataaccc tgataaatgc ttcaattata ttgaaaaagg
     4021 aagagtatga gtatccaaca tttccgtgtc gcccttattc ccttttttgc ggccttctgc
     4081 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4141 ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt
     4201 cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg
     4261 ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4321 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4381 gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4441 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4501 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4561 acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga actacttact
     4621 cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt
     4681 ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4741 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4801 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4861 ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc
     4921 ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct
     4981 cttaggcggt ccgattagcg acatagtcat caat
//
