LOCUS       sequence-181828-        5007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: PvUbi1, Gene: GFP, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3012
FEATURES             Location/Qualifiers
     source          1..5007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1589..1600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2901..2929)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3524..3544
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      3595..3843
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3915..4019
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccga agagcacgtc agtgtttggt
      901 ttccactagc acgagtagcg caatcagaaa attttcaatg catgaagtac taaacgaagt
      961 ttatttagaa atttttttaa gaaatgagtg taattttttg cgacgaattt aatgacaata
     1021 attaatcgat gattgcctac agtaatgcta cagtaaccaa cctctaatca tgcgtcgaat
     1081 gcgtcattag attcgtctcg caaaatagca caagaattat gaaattaatt ttacaaacta
     1141 tttttattta atactaataa ttaactgtca aagtttgtgc tactcgcaag agtagcgcga
     1201 accaaacacg gcctggagga gcacggtaac ggcgtcgaca aactaacggc caccacccgc
     1261 caacgcaaag gagacggatg agagttgact tcttgacggt tctccacccc tctgtctctc
     1321 tgtcactggg ccctgggtcc ccctctcgaa agttcctctg gccgaaattg cgcggcggag
     1381 acgaggcggg cggaaccgtc acggcagagg attccttccc caccctgcct ggcccggcca
     1441 tatataaaca gccaccgccc ctccccgttc cccatcgcgt ctcgtctcgt gttgttccca
     1501 gaacacaacc aaaatccaaa tcctcctcct cctcccgagc ctcgtcgatc cctcacccgc
     1561 ttcaaggtac ggcgatcctc ctctcccttc tcccctcgat cgattatgcg tgttccgttt
     1621 ccgtttccga tcgagcgaat cgatggttag gacccatggg ggacccatgg ggtgtcgtgt
     1681 ggtggtctgg tttgatccgc gatatttctc cgttcgtagt gtagatctga tcgaatccct
     1741 ggtgaaatcg ttgatcgtgc tattcgtgtg agggttctta ggtttggagt tgtggaggta
     1801 gttctgatcg gtttgtaggt gagattttcc ccatgatttt gcttggctcg tttgtcttgg
     1861 ttagattaga tctgcccgca ttttgttcga tatttctgat gcagatatga tgaataattt
     1921 cgtccttgta tcccgcgtcc gtatgtgtat taagtttgca ggtcctagtt aggtttttcc
     1981 tactgatttg tcttatccat tctgtttagc ttgcaaggtt tggtaatggt ccggcatgtt
     2041 tgtctctata gattagagta gaataagatt atctcaacaa gctgttggct tatcaatttt
     2101 ggatctgcat gtgtttcgca tctatatctt tgcaattaag atggtagatg gacatatgct
     2161 cctgttgagt tgatgttgta ccttttacct gaggtctgag gaacatgcat cctcctgcta
     2221 ctttgtgctt atacagatca tcaagattat gtagctaata ttcgatcagt ttctagtatc
     2281 tacatggtaa acttgcatgc acttgctact tatttttgat atacttggat gataacatat
     2341 gctgctggtt gattcctacc tacatgatga acattttaca ggccattagt gtctgtctgt
     2401 atgtgttgtt cctgtttgct tcagtctatt tctgtttcat tcctagttta ttggttctct
     2461 gctagatact taccctgctg ggcttagtta tcatcttatc tcgaatgcat tttcatgttt
     2521 atagatgaat atacactcag ataggtgtag atgtatgcta ctgtttctct acgttgctgt
     2581 aggttttacc tgtggcaact gcatactcct gttgcttcgc tagatatgta tgtgcttata
     2641 tagattaaga tatgtgtgat ggttctttag tatatctgat gatcatgtat gctcttttaa
     2701 cttcttgcta cacttggtaa catgctgtga tgctgtttgt tgattctgta gcactaccaa
     2761 tgatgacctt atctctcttt gtatatgatg tttctgtttg tttgaggctt gtgttactgc
     2821 tagttactta ccctgttgcc tggctaatct tctgcagatc ctgcaggatg gtgagtaaag
     2881 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     2941 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     3001 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaaccctg gtcaccaccc
     3061 tgacctacgg cgtgcagtgc ttctcccgtt accctgatca tatgaagcgg cacgacttct
     3121 tcaagagcgc catgcctgag ggatacgtgc aggagaggac catcttcttc aaggacgacg
     3181 ggaactacaa gacacgtgct gaagtcaagt ttgagggaga caccctcgtc aacaggatcg
     3241 agcttaaggg aatcgatttc aaggaggacg gaaacatcct cggccacaag ttggaataca
     3301 actacaactc ccacaacgta tacatcatgg ccgacaagca aaagaacggc atcaaagcca
     3361 acttcaagac ccgccacaac atcgaagacg gcggcgtgca actcgctgat cattatcaac
     3421 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     3481 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     3541 taacagctgc tgggattaca catggcatgg atgaactata caaataactc gagatatgaa
     3601 gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt
     3661 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat
     3721 cacattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc
     3781 ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat
     3841 aaggagctcc ggtgacggac gtgggcaggt gatgaaaagc cttagggtca ggtggcactt
     3901 ttcggggaaa tgtgcgatga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3961 atccgctaat gagacaataa ccctgataaa tgcttcaatt atattgaaaa aggaagagta
     4021 tgagtatcca acatttccgt gtcgccctta ttcccttttt tgcggccttc tgccttcctg
     4081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc
     4141 gagtgggtta cattgaactg gatctcaaca gcggcaaaat cttagagagt tttcgccccg
     4201 aagaacgctt cccaatgatg agcactttca aagttctgct atgtggcgcg gtgttatccc
     4261 gtattgatgc tgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4321 ttgagtattc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4381 gtagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg
     4441 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4501 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4561 ctgtagcaat ggcaacaact ctccgcaaac tattaactgg cgaactactt actcttgctt
     4621 cacgccagca actcattgac tggatggagg cggacaaagt tgcaggacca cttctgcgct
     4681 cggcacttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4861 cactgattaa gcattggtaa ctgtcagacg aagggaaata aatagtagcc cgcctgatgt
     4921 gcgggctttt ttttggtggt ggttaccagt gtcgcttagc taccgtaggt cctcttaggc
     4981 ggtccgatta gcgacatagt catcaat
//
