LOCUS       sequence-181829-        4889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: PvUbi2, Gene: GFP, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3013
FEATURES             Location/Qualifiers
     source          1..4889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2783..2811)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3406..3426
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      3477..3725
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        3797..3901
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc ggcgcgccga agccaactaa acaagaccat
      901 aaccatggtg acatttgaca tagttgttta ctacttgctt gagccccacc cttgcttatc
      961 ggttgaacat tacaagatac actgcgggtg gcctaaggca caccgtccga aaccggcaaa
     1021 ccaagcctga tcgccgaaat ccaaaatcac taccggcaat ctctaaagtt tatttcatcc
     1081 ttatatgacg aggaaagaaa agaagagaga aataatatct taacttctaa atcagtcgcg
     1141 tcaactttct cggctaagaa agtgagcact atcatttcga agaccatgtc atgagtgccg
     1201 acttgccata tcttattata ttcttattta tttaattata atcccattgc aatacgtcta
     1261 ttctatcatg gcctgccact aacgctccgt ctaacgtcgt taagccattg tcataagcgg
     1321 ctgctcaaaa ctcttcccgg tggaggcgag gcgttaacgg cgtctacaaa tctaacggcc
     1381 accaaccatc cagccgcctc tcgaaagctc cgctccgatc gcggaaattg cgtggcggag
     1441 acgagcgggc tcctctcaca cggcccggaa ccgtcacggc acgggtgggg gattccttcc
     1501 ccaaccctcc ccacctctcc tccccccgtc gcagcccata aatacagggc cctccgcgcc
     1561 tcttcccaca atctcacatc gtctcatcgt tcggagcgca caacccccgg gttccaaatc
     1621 caaattgctc tcctcgcgac cctcggcgat ccttcccccg cttcaaggta cggcgatcgt
     1681 ctcccccgtc ctcttgcccc atctcctcgc tcggcgtggt ttggtggttc tgcttggtct
     1741 gtggctagga actaggctga ggcgttgacg aaatcatgct agatccgcgt gtttcctgat
     1801 cgtgggtggc tgggaggtgg ggttttcgtg tagatctgat cggttccgct gtttatcctg
     1861 tcatgctcat gtgatttgtg gggattttag gtcgtttgtc cgggaatcgt ggggttgctt
     1921 ctaggctgtt cgtagatgag atcgttctca cgatctgctg ggtcgctgcc taggttcagc
     1981 taggtctgcc ctgtttttgg gttcgttttc gggatctgta cgtgcatcta ttatctggtt
     2041 cgatggtgct agctaggaac aaacaactga ttcgtccgat cgattgtttt gttgccatgt
     2101 gcaaggttag gtcgttatct gattgctgta gatcagagta gaataagatc atcacaagct
     2161 agctcttggg cttattatga atctgcgttt gttgcatgat taagatgatt atgctttttc
     2221 ttatgctgcc gtttgtatat gatgcggtag cttttaactg aatagcacac ctttcctgtt
     2281 tagttagatt agattagatt gcatgataga tgaggatata tgctgctaca tcagtttgat
     2341 gattctctgg tacctcataa tcaactagct catgtgctta aattgaaact gcatgtgcca
     2401 catgattaag atgctaagat tggtgaagat atatacgctg ctgttcctat aggatcctgt
     2461 agcttttacc tggtcaacat gcatcgtcct gttatggata gatatgcatg atagatgaag
     2521 atatgtactg ctacaatttg atgattcttt tgtgcacctg atgatcatgc atgctctttg
     2581 cccttacttt gatatacttg gatgatggca tgcttagtac taatgatgtg atgaacacac
     2641 atgacctgtt ggtatgaata tgatgttgct gtttgcttgt gatgagttct gtttgtttac
     2701 tgctaggcac ttaccctgtt gtctggttct cttttgcaga acctgcagga tggtgagtaa
     2761 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     2821 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     2881 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaaccc tggtcaccac
     2941 cctgacctac ggcgtgcagt gcttctcccg ttaccctgat catatgaagc ggcacgactt
     3001 cttcaagagc gccatgcctg agggatacgt gcaggagagg accatcttct tcaaggacga
     3061 cgggaactac aagacacgtg ctgaagtcaa gtttgaggga gacaccctcg tcaacaggat
     3121 cgagcttaag ggaatcgatt tcaaggagga cggaaacatc ctcggccaca agttggaata
     3181 caactacaac tcccacaacg tatacatcat ggccgacaag caaaagaacg gcatcaaagc
     3241 caacttcaag acccgccaca acatcgaaga cggcggcgtg caactcgctg atcattatca
     3301 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     3361 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     3421 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataac tcgagatatg
     3481 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     3541 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     3601 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     3661 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     3721 ataaggagct ccggtgacgg acgtgggcag gtgatgaaaa gccttagggt caggtggcac
     3781 ttttcgggga aatgtgcgat gaacccctat ttgtttattt ttctaaatac attcaaatat
     3841 gtatccgcta atgagacaat aaccctgata aatgcttcaa ttatattgaa aaaggaagag
     3901 tatgagtatc caacatttcc gtgtcgccct tattcccttt tttgcggcct tctgccttcc
     3961 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4021 ccgagtgggt tacattgaac tggatctcaa cagcggcaaa atcttagaga gttttcgccc
     4081 cgaagaacgc ttcccaatga tgagcacttt caaagttctg ctatgtggcg cggtgttatc
     4141 ccgtattgat gctgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4201 ggttgagtat tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4261 atgtagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4321 tggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4381 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4441 gcctgtagca atggcaacaa ctctccgcaa actattaact ggcgaactac ttactcttgc
     4501 ttcacgccag caactcattg actggatgga ggcggacaaa gttgcaggac cacttctgcg
     4561 ctcggcactt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4621 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4681 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4741 ctcactgatt aagcattggt aactgtcaga cgaagggaaa taaatagtag cccgcctgat
     4801 gtgcgggctt ttttttggtg gtggttacca gtgtcgctta gctaccgtag gtcctcttag
     4861 gcggtccgat tagcgacata gtcatcaat
//
