LOCUS       sequence-181831-        3995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3001
FEATURES             Location/Qualifiers
     source          1..3995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1374..1719
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1662..1682
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     primer_bind     complement(1770..1798)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2393..2413
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2903..3007
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccagat ttgccttttc
      901 aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc aggtatcatc
      961 aagacgatct acccgagcaa taatctccag gaaatcaaat accttcccaa gaaggttaaa
     1021 gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga tatatttctc
     1081 aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcacaa accaaggcaa
     1141 gtaatagaga ttggagtctc taaaaaggta gttcccactg aatcaaaggc catggagtca
     1201 aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca gttcatacag
     1261 agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga gcacgacaca
     1321 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact
     1381 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac
     1441 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa
     1501 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
     1561 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
     1621 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct
     1681 tcctctatat aaggaagttc atttcatttg gagagaacac gggggactcc tgcaggatgg
     1741 tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
     1801 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1861 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaaccctgg
     1921 tcaccaccct gacctacggc gtgcagtgct tctcccgtta ccctgatcat atgaagcggc
     1981 acgacttctt caagagcgcc atgcctgagg gatacgtgca ggagaggacc atcttcttca
     2041 aggacgacgg gaactacaag acacgtgctg aagtcaagtt tgagggagac accctcgtca
     2101 acaggatcga gcttaaggga atcgatttca aggaggacgg aaacatcctc ggccacaagt
     2161 tggaatacaa ctacaactcc cacaacgtat acatcatggc cgacaagcaa aagaacggca
     2221 tcaaagccaa cttcaagacc cgccacaaca tcgaagacgg cggcgtgcaa ctcgctgatc
     2281 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     2341 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     2401 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataactcg
     2461 aggttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
     2521 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
     2581 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
     2641 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
     2701 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt
     2761 gtagttgtga gctcgcgcgt agtcctcggt aatatcgcag aacaaaagta cctgatatcg
     2821 agtgtacttc aagtcacacc ggcgagtgac tagcaggtga tgaaaagcct tagggtcagg
     2881 tggcactttt cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc
     2941 aaatatgtat ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag
     3001 gaagagtatg agtatccaac atttccgtgt cgcccttatt cccttttttg cggccttctg
     3061 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     3121 gggtgcccga gtgggttaca ttgaactgga tctcaacagc ggcaaaatct tagagagttt
     3181 tcgccccgaa gaacgcttcc caatgatgag cactttcaaa gttctgctat gtggcgcggt
     3241 gttatcccgt attgatgctg ggcaagagca actcggtcgc cgcatacact attctcagaa
     3301 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     3361 agaattatgt agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     3421 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     3481 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     3541 cacgatgcct gtagcaatgg caacaactct ccgcaaacta ttaactggcg aactacttac
     3601 tcttgcttca cgccagcaac tcattgactg gatggaggcg gacaaagttg caggaccact
     3661 tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     3721 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3781 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3841 aggtgcctca ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg
     3901 cctgatgtgc gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc
     3961 tcttaggcgg tccgattagc gacatagtca tcaat
//
