LOCUS       sequence-181832-        3618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: CmYLCV, Gene: GFP (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3003
FEATURES             Location/Qualifiers
     source          1..3618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1393..1421)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2016..2036
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2526..2630
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgcctggc agacatactg
      901 tcccacaaat gaagatggaa tctgtaaaag aaaacgcgtg aaataatgcg tctgacaaag
      961 gttaggtcgg ctgcctttaa tcaataccaa agtggtccct accacgatgg aaaaactgtg
     1021 cagtcggttt ggctttttct gacgaacaaa taagattcgt ggccgacagg tgggggtcca
     1081 ccatgtgaag gcatcttcag actccaataa tggagcaatg acgtaagggc ttacgaaata
     1141 agtaagggta gtttgggaaa tgtccactca cccgtcagtc tataaatact tagcccctcc
     1201 ctcattgtta agggagcaaa atctcagaga gatagtccta gagagagaaa gagagcaagt
     1261 agcctagaag tagtcaaggc ggcgaagtat tcaggcacgt ggccaggaag aagaaaagcc
     1321 aagacgacga aaacaggtaa gagctaagct tcctgcagga tggtgagtaa aggagaagaa
     1381 cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa
     1441 ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt
     1501 atttgcacta ctggaaaact acctgttcca tggccaaccc tggtcaccac cctgacctac
     1561 ggcgtgcagt gcttctcccg ttaccctgat catatgaagc ggcacgactt cttcaagagc
     1621 gccatgcctg agggatacgt gcaggagagg accatcttct tcaaggacga cgggaactac
     1681 aagacacgtg ctgaagtcaa gtttgaggga gacaccctcg tcaacaggat cgagcttaag
     1741 ggaatcgatt tcaaggagga cggaaacatc ctcggccaca agttggaata caactacaac
     1801 tcccacaacg tatacatcat ggccgacaag caaaagaacg gcatcaaagc caacttcaag
     1861 acccgccaca acatcgaaga cggcggcgtg caactcgctg atcattatca acaaaatact
     1921 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     1981 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     2041 gctgggatta cacatggcat ggatgaacta tacaaataac tcgaggttcg agtattatgg
     2101 cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta
     2161 ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata
     2221 tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt
     2281 tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc
     2341 gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg tgagctcgcg
     2401 cgtagtcctc ggtaatatcg cagaacaaaa gtacctgata tcgagtgtac ttcaagtcac
     2461 accggcgagt gactagcagg tgatgaaaag ccttagggtc aggtggcact tttcggggaa
     2521 atgtgcgatg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctaa
     2581 tgagacaata accctgataa atgcttcaat tatattgaaa aaggaagagt atgagtatcc
     2641 aacatttccg tgtcgccctt attccctttt ttgcggcctt ctgccttcct gtttttgctc
     2701 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgcc cgagtgggtt
     2761 acattgaact ggatctcaac agcggcaaaa tcttagagag ttttcgcccc gaagaacgct
     2821 tcccaatgat gagcactttc aaagttctgc tatgtggcgc ggtgttatcc cgtattgatg
     2881 ctgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtatt
     2941 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgtagtgctg
     3001 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatt ggaggaccga
     3061 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     3121 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     3181 tggcaacaac tctccgcaaa ctattaactg gcgaactact tactcttgct tcacgccagc
     3241 aactcattga ctggatggag gcggacaaag ttgcaggacc acttctgcgc tcggcacttc
     3301 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     3361 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     3421 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     3481 agcattggta actgtcagac gaagggaaat aaatagtagc ccgcctgatg tgcgggcttt
     3541 tttttggtgg tggttaccag tgtcgcttag ctaccgtagg tcctcttagg cggtccgatt
     3601 agcgacatag tcatcaat
//
