LOCUS       sequence-181833-        3660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: FMV 34S, Gene: GFP (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3006
FEATURES             Location/Qualifiers
     source          1..3660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1435..1463)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2058..2078
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2568..2672
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccagct ggcttgtggg
      901 gaccagacaa aaaaggaatg gtgcagaatt gttaggcgca cctaccaaaa gcatctttgc
      961 ctttattgca aagataaagc agattcctct agtacaagtg gggaacaaaa taacgtggaa
     1021 aagagctgtc ctgacagccc actcactaat gcgtatgacg aacgcagtga cgaccacaaa
     1081 acagctggct tgtggggacc agacaaaaaa ggaatggtgc agaattgtta ggcgcaccta
     1141 ccaaaagcat ctttgccttt attgcaaaga taaagcagat tcctctagta caagtgggga
     1201 acaaaataac gtggaaaaga gctgtcctga cagcccactc actaatgcgt atgacgaacg
     1261 cagtgacgac cacaaaagaa ttccctctat ataagaaggc attcattccc atttgaagga
     1321 tcatcagata ctgaaccaat atttctcact ctaagaaatt aagagctttg tattcttcaa
     1381 tgagaggcta agacctgcag gatggtgagt aaaggagaag aacttttcac tggagttgtc
     1441 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     1501 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     1561 ctacctgttc catggccaac cctggtcacc accctgacct acggcgtgca gtgcttctcc
     1621 cgttaccctg atcatatgaa gcggcacgac ttcttcaaga gcgccatgcc tgagggatac
     1681 gtgcaggaga ggaccatctt cttcaaggac gacgggaact acaagacacg tgctgaagtc
     1741 aagtttgagg gagacaccct cgtcaacagg atcgagctta agggaatcga tttcaaggag
     1801 gacggaaaca tcctcggcca caagttggaa tacaactaca actcccacaa cgtatacatc
     1861 atggccgaca agcaaaagaa cggcatcaaa gccaacttca agacccgcca caacatcgaa
     1921 gacggcggcg tgcaactcgc tgatcattat caacaaaata ctccaattgg cgatggccct
     1981 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     2041 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     2101 atggatgaac tatacaaata actcgaggtt cgagtattat ggcattggga aaactgtttt
     2161 tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga
     2221 actgtgaaat ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc
     2281 tcaaattaat attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag
     2341 agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga
     2401 agttaatatg aggagtaaaa cacttgtagt tgtgagctcg cgcgtagtcc tcggtaatat
     2461 cgcagaacaa aagtacctga tatcgagtgt acttcaagtc acaccggcga gtgactagca
     2521 ggtgatgaaa agccttaggg tcaggtggca cttttcgggg aaatgtgcga tgaaccccta
     2581 tttgtttatt tttctaaata cattcaaata tgtatccgct aatgagacaa taaccctgat
     2641 aaatgcttca attatattga aaaaggaaga gtatgagtat ccaacatttc cgtgtcgccc
     2701 ttattccctt ttttgcggcc ttctgccttc ctgtttttgc tcacccagaa acgctggtga
     2761 aagtaaaaga tgctgaagat cagttgggtg cccgagtggg ttacattgaa ctggatctca
     2821 acagcggcaa aatcttagag agttttcgcc ccgaagaacg cttcccaatg atgagcactt
     2881 tcaaagttct gctatgtggc gcggtgttat cccgtattga tgctgggcaa gagcaactcg
     2941 gtcgccgcat acactattct cagaatgact tggttgagta ttcaccagtc acagaaaagc
     3001 atcttacgga tggcatgaca gtaagagaat tatgtagtgc tgccataacc atgagtgata
     3061 acactgcggc caacttactt ctgacaacga ttggaggacc gaaggagcta accgcttttt
     3121 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     3181 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca actctccgca
     3241 aactattaac tggcgaacta cttactcttg cttcacgcca gcaactcatt gactggatgg
     3301 aggcggacaa agttgcagga ccacttctgc gctcggcact tccggctggc tggtttattg
     3361 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3421 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3481 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3541 acgaagggaa ataaatagta gcccgcctga tgtgcgggct tttttttggt ggtggttacc
     3601 agtgtcgctt agctaccgta ggtcctctta ggcggtccga ttagcgacat agtcatcaat
//
