LOCUS       sequence-181835-        5141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: ZmUbi, Gene: GFP (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3010
FEATURES             Location/Qualifiers
     source          1..5141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2916..2944)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3539..3559
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        4049..4153
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgcctgca gtgcagcgtg
      901 acccggtcgt gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta
      961 ccacatattt tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat
     1021 ttaaacttta ctctacgaat aatataatct atagtactac aataatatca gtgttttaga
     1081 gaatcatata aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag
     1141 gactctacag ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt
     1201 cacctatata atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg
     1261 tttttataga ctaatttttt tagtacatct attttattct attttagcct ctaaattaag
     1321 aaaactaaaa ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa
     1381 taaagtgact aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt
     1441 tttcttgttt cgagtagata atgccagcct gttaaacgcc gtcgacgagt ctaacggaca
     1501 ccaaccagcg aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct
     1561 gtcgctgcct ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc
     1621 ggcatccaga aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct
     1681 cctcctctca cggcaccggc agctacgggg gattcctttc ccaccgctcc ttcgctttcc
     1741 cttcctcgcc cgccgtaata aatagacacc ccctccacac cctctttccc caacctcgtg
     1801 ttgttcggag cgcacacaca cacaaccaga tctcccccaa atccacccgt cggcacctcc
     1861 gcttcaaggt acgccgctcg tcctcccccc ccccctctct accttctcta gatcggcgtt
     1921 ccggtccatg gttagggccc ggtagttcta cttctgttca tgtttgtgtt agatccgtgt
     1981 ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc gacctgtacg tcagacacgt
     2041 tctgattgct aacttgccag tgtttctctt tggggaatcc tgggatggct ctagccgttc
     2101 cgcagacggg atcgatttca tgattttttt tgtttcgttg catagggttt ggtttgccct
     2161 tttcctttat ttcaatatat gccgtgcact tgtttgtcgg gtcatctttt catgcttttt
     2221 tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg ttctagatcg gagtagaatt
     2281 aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata
     2341 catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac
     2401 atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga
     2461 tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca
     2521 aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt
     2581 tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt
     2641 ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt
     2701 acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga
     2761 tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta
     2821 tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttcctgca
     2881 ggatggtgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     2941 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     3001 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa
     3061 ccctggtcac caccctgacc tacggcgtgc agtgcttctc ccgttaccct gatcatatga
     3121 agcggcacga cttcttcaag agcgccatgc ctgagggata cgtgcaggag aggaccatct
     3181 tcttcaagga cgacgggaac tacaagacac gtgctgaagt caagtttgag ggagacaccc
     3241 tcgtcaacag gatcgagctt aagggaatcg atttcaagga ggacggaaac atcctcggcc
     3301 acaagttgga atacaactac aactcccaca acgtatacat catggccgac aagcaaaaga
     3361 acggcatcaa agccaacttc aagacccgcc acaacatcga agacggcggc gtgcaactcg
     3421 ctgatcatta tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc
     3481 attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg
     3541 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat
     3601 aactcgaggt tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg
     3661 cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga
     3721 tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt
     3781 tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt
     3841 tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa
     3901 acacttgtag ttgtgagctc gcgcgtagtc ctcggtaata tcgcagaaca aaagtacctg
     3961 atatcgagtg tacttcaagt cacaccggcg agtgactagc aggtgatgaa aagccttagg
     4021 gtcaggtggc acttttcggg gaaatgtgcg atgaacccct atttgtttat ttttctaaat
     4081 acattcaaat atgtatccgc taatgagaca ataaccctga taaatgcttc aattatattg
     4141 aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc cttattccct tttttgcggc
     4201 cttctgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4261 tcagttgggt gcccgagtgg gttacattga actggatctc aacagcggca aaatcttaga
     4321 gagttttcgc cccgaagaac gcttcccaat gatgagcact ttcaaagttc tgctatgtgg
     4381 cgcggtgtta tcccgtattg atgctgggca agagcaactc ggtcgccgca tacactattc
     4441 tcagaatgac ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac
     4501 agtaagagaa ttatgtagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     4561 tctgacaacg attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     4621 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     4681 tgacaccacg atgcctgtag caatggcaac aactctccgc aaactattaa ctggcgaact
     4741 acttactctt gcttcacgcc agcaactcat tgactggatg gaggcggaca aagttgcagg
     4801 accacttctg cgctcggcac ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4861 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4921 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4981 tgagataggt gcctcactga ttaagcattg gtaactgtca gacgaaggga aataaatagt
     5041 agcccgcctg atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt
     5101 aggtcctctt aggcggtccg attagcgaca tagtcatcaa t
//
