LOCUS       sequence-181836-        5134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: PvUbi1, Gene: GFP (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5134)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3012
FEATURES             Location/Qualifiers
     source          1..5134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1597..1608)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2909..2937)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3532..3552
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        4042..4146
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccgaag agcacgtcag
      901 tgtttggttt ccactagcac gagtagcgca atcagaaaat tttcaatgca tgaagtacta
      961 aacgaagttt atttagaaat ttttttaaga aatgagtgta attttttgcg acgaatttaa
     1021 tgacaataat taatcgatga ttgcctacag taatgctaca gtaaccaacc tctaatcatg
     1081 cgtcgaatgc gtcattagat tcgtctcgca aaatagcaca agaattatga aattaatttt
     1141 acaaactatt tttatttaat actaataatt aactgtcaaa gtttgtgcta ctcgcaagag
     1201 tagcgcgaac caaacacggc ctggaggagc acggtaacgg cgtcgacaaa ctaacggcca
     1261 ccacccgcca acgcaaagga gacggatgag agttgacttc ttgacggttc tccacccctc
     1321 tgtctctctg tcactgggcc ctgggtcccc ctctcgaaag ttcctctggc cgaaattgcg
     1381 cggcggagac gaggcgggcg gaaccgtcac ggcagaggat tccttcccca ccctgcctgg
     1441 cccggccata tataaacagc caccgcccct ccccgttccc catcgcgtct cgtctcgtgt
     1501 tgttcccaga acacaaccaa aatccaaatc ctcctcctcc tcccgagcct cgtcgatccc
     1561 tcacccgctt caaggtacgg cgatcctcct ctcccttctc ccctcgatcg attatgcgtg
     1621 ttccgtttcc gtttccgatc gagcgaatcg atggttagga cccatggggg acccatgggg
     1681 tgtcgtgtgg tggtctggtt tgatccgcga tatttctccg ttcgtagtgt agatctgatc
     1741 gaatccctgg tgaaatcgtt gatcgtgcta ttcgtgtgag ggttcttagg tttggagttg
     1801 tggaggtagt tctgatcggt ttgtaggtga gattttcccc atgattttgc ttggctcgtt
     1861 tgtcttggtt agattagatc tgcccgcatt ttgttcgata tttctgatgc agatatgatg
     1921 aataatttcg tccttgtatc ccgcgtccgt atgtgtatta agtttgcagg tcctagttag
     1981 gtttttccta ctgatttgtc ttatccattc tgtttagctt gcaaggtttg gtaatggtcc
     2041 ggcatgtttg tctctataga ttagagtaga ataagattat ctcaacaagc tgttggctta
     2101 tcaattttgg atctgcatgt gtttcgcatc tatatctttg caattaagat ggtagatgga
     2161 catatgctcc tgttgagttg atgttgtacc ttttacctga ggtctgagga acatgcatcc
     2221 tcctgctact ttgtgcttat acagatcatc aagattatgt agctaatatt cgatcagttt
     2281 ctagtatcta catggtaaac ttgcatgcac ttgctactta tttttgatat acttggatga
     2341 taacatatgc tgctggttga ttcctaccta catgatgaac attttacagg ccattagtgt
     2401 ctgtctgtat gtgttgttcc tgtttgcttc agtctatttc tgtttcattc ctagtttatt
     2461 ggttctctgc tagatactta ccctgctggg cttagttatc atcttatctc gaatgcattt
     2521 tcatgtttat agatgaatat acactcagat aggtgtagat gtatgctact gtttctctac
     2581 gttgctgtag gttttacctg tggcaactgc atactcctgt tgcttcgcta gatatgtatg
     2641 tgcttatata gattaagata tgtgtgatgg ttctttagta tatctgatga tcatgtatgc
     2701 tcttttaact tcttgctaca cttggtaaca tgctgtgatg ctgtttgttg attctgtagc
     2761 actaccaatg atgaccttat ctctctttgt atatgatgtt tctgtttgtt tgaggcttgt
     2821 gttactgcta gttacttacc ctgttgcctg gctaatcttc tgcagatcct gcaggatggt
     2881 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     2941 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     3001 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caaccctggt
     3061 caccaccctg acctacggcg tgcagtgctt ctcccgttac cctgatcata tgaagcggca
     3121 cgacttcttc aagagcgcca tgcctgaggg atacgtgcag gagaggacca tcttcttcaa
     3181 ggacgacggg aactacaaga cacgtgctga agtcaagttt gagggagaca ccctcgtcaa
     3241 caggatcgag cttaagggaa tcgatttcaa ggaggacgga aacatcctcg gccacaagtt
     3301 ggaatacaac tacaactccc acaacgtata catcatggcc gacaagcaaa agaacggcat
     3361 caaagccaac ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac tcgctgatca
     3421 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     3481 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     3541 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataactcga
     3601 ggttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     3661 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     3721 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     3781 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     3841 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacacttg
     3901 tagttgtgag ctcgcgcgta gtcctcggta atatcgcaga acaaaagtac ctgatatcga
     3961 gtgtacttca agtcacaccg gcgagtgact agcaggtgat gaaaagcctt agggtcaggt
     4021 ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt tatttttcta aatacattca
     4081 aatatgtatc cgctaatgag acaataaccc tgataaatgc ttcaattata ttgaaaaagg
     4141 aagagtatga gtatccaaca tttccgtgtc gcccttattc ccttttttgc ggccttctgc
     4201 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4261 ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt
     4321 cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg
     4381 ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4441 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4501 gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4561 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4621 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4681 acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga actacttact
     4741 cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt
     4801 ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4861 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4921 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4981 ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc
     5041 ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct
     5101 cttaggcggt ccgattagcg acatagtcat caat
//
