LOCUS       sequence-181837-        5016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: PvUbi2, Gene: GFP (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3013
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2791..2819)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3414..3434
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        3924..4028
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccgaag ccaactaaac
      901 aagaccataa ccatggtgac atttgacata gttgtttact acttgcttga gccccaccct
      961 tgcttatcgg ttgaacatta caagatacac tgcgggtggc ctaaggcaca ccgtccgaaa
     1021 ccggcaaacc aagcctgatc gccgaaatcc aaaatcacta ccggcaatct ctaaagttta
     1081 tttcatcctt atatgacgag gaaagaaaag aagagagaaa taatatctta acttctaaat
     1141 cagtcgcgtc aactttctcg gctaagaaag tgagcactat catttcgaag accatgtcat
     1201 gagtgccgac ttgccatatc ttattatatt cttatttatt taattataat cccattgcaa
     1261 tacgtctatt ctatcatggc ctgccactaa cgctccgtct aacgtcgtta agccattgtc
     1321 ataagcggct gctcaaaact cttcccggtg gaggcgaggc gttaacggcg tctacaaatc
     1381 taacggccac caaccatcca gccgcctctc gaaagctccg ctccgatcgc ggaaattgcg
     1441 tggcggagac gagcgggctc ctctcacacg gcccggaacc gtcacggcac gggtggggga
     1501 ttccttcccc aaccctcccc acctctcctc cccccgtcgc agcccataaa tacagggccc
     1561 tccgcgcctc ttcccacaat ctcacatcgt ctcatcgttc ggagcgcaca acccccgggt
     1621 tccaaatcca aattgctctc ctcgcgaccc tcggcgatcc ttcccccgct tcaaggtacg
     1681 gcgatcgtct cccccgtcct cttgccccat ctcctcgctc ggcgtggttt ggtggttctg
     1741 cttggtctgt ggctaggaac taggctgagg cgttgacgaa atcatgctag atccgcgtgt
     1801 ttcctgatcg tgggtggctg ggaggtgggg ttttcgtgta gatctgatcg gttccgctgt
     1861 ttatcctgtc atgctcatgt gatttgtggg gattttaggt cgtttgtccg ggaatcgtgg
     1921 ggttgcttct aggctgttcg tagatgagat cgttctcacg atctgctggg tcgctgccta
     1981 ggttcagcta ggtctgccct gtttttgggt tcgttttcgg gatctgtacg tgcatctatt
     2041 atctggttcg atggtgctag ctaggaacaa acaactgatt cgtccgatcg attgttttgt
     2101 tgccatgtgc aaggttaggt cgttatctga ttgctgtaga tcagagtaga ataagatcat
     2161 cacaagctag ctcttgggct tattatgaat ctgcgtttgt tgcatgatta agatgattat
     2221 gctttttctt atgctgccgt ttgtatatga tgcggtagct tttaactgaa tagcacacct
     2281 ttcctgttta gttagattag attagattgc atgatagatg aggatatatg ctgctacatc
     2341 agtttgatga ttctctggta cctcataatc aactagctca tgtgcttaaa ttgaaactgc
     2401 atgtgccaca tgattaagat gctaagattg gtgaagatat atacgctgct gttcctatag
     2461 gatcctgtag cttttacctg gtcaacatgc atcgtcctgt tatggataga tatgcatgat
     2521 agatgaagat atgtactgct acaatttgat gattcttttg tgcacctgat gatcatgcat
     2581 gctctttgcc cttactttga tatacttgga tgatggcatg cttagtacta atgatgtgat
     2641 gaacacacat gacctgttgg tatgaatatg atgttgctgt ttgcttgtga tgagttctgt
     2701 ttgtttactg ctaggcactt accctgttgt ctggttctct tttgcagaac ctgcaggatg
     2761 gtgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     2821 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     2881 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaaccctg
     2941 gtcaccaccc tgacctacgg cgtgcagtgc ttctcccgtt accctgatca tatgaagcgg
     3001 cacgacttct tcaagagcgc catgcctgag ggatacgtgc aggagaggac catcttcttc
     3061 aaggacgacg ggaactacaa gacacgtgct gaagtcaagt ttgagggaga caccctcgtc
     3121 aacaggatcg agcttaaggg aatcgatttc aaggaggacg gaaacatcct cggccacaag
     3181 ttggaataca actacaactc ccacaacgta tacatcatgg ccgacaagca aaagaacggc
     3241 atcaaagcca acttcaagac ccgccacaac atcgaagacg gcggcgtgca actcgctgat
     3301 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     3361 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     3421 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataactc
     3481 gaggttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt
     3541 aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag
     3601 aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt
     3661 ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga
     3721 gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact
     3781 tgtagttgtg agctcgcgcg tagtcctcgg taatatcgca gaacaaaagt acctgatatc
     3841 gagtgtactt caagtcacac cggcgagtga ctagcaggtg atgaaaagcc ttagggtcag
     3901 gtggcacttt tcggggaaat gtgcgatgaa cccctatttg tttatttttc taaatacatt
     3961 caaatatgta tccgctaatg agacaataac cctgataaat gcttcaatta tattgaaaaa
     4021 ggaagagtat gagtatccaa catttccgtg tcgcccttat tccctttttt gcggccttct
     4081 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4141 tgggtgcccg agtgggttac attgaactgg atctcaacag cggcaaaatc ttagagagtt
     4201 ttcgccccga agaacgcttc ccaatgatga gcactttcaa agttctgcta tgtggcgcgg
     4261 tgttatcccg tattgatgct gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4321 atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4381 gagaattatg tagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4441 caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4501 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4561 ccacgatgcc tgtagcaatg gcaacaactc tccgcaaact attaactggc gaactactta
     4621 ctcttgcttc acgccagcaa ctcattgact ggatggaggc ggacaaagtt gcaggaccac
     4681 ttctgcgctc ggcacttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4741 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4801 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4861 taggtgcctc actgattaag cattggtaac tgtcagacga agggaaataa atagtagccc
     4921 gcctgatgtg cgggcttttt tttggtggtg gttaccagtg tcgcttagct accgtaggtc
     4981 ctcttaggcg gtccgattag cgacatagtc atcaat
//
