LOCUS       sequence-181842-       10069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: dTaCas9-VPR, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10069)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A3910
FEATURES             Location/Qualifiers
     source          1..10069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(2892..2918,2919..2921,2922..5408,5409..5411,
                     5412..6995)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6996..7043
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7059..7208
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7233..7253
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7683..8039
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8058..8627
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      8649..8897
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        8977..9081
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc tgcaggcgcg cctgcagtgc agcgtgaccc
      901 ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac
      961 atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa
     1021 actttactct acgaataata taatctatag tactacaata atatcagtgt tttagagaat
     1081 catataaatg aacagttaga catggtctaa aggacaattg agtattttga caacaggact
     1141 ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc
     1201 tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt
     1261 tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa
     1321 ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa
     1381 gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc
     1441 ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa
     1501 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg
     1561 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca
     1621 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc
     1681 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc
     1741 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt
     1801 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt
     1861 caaggtacgc cgctcgtcct cccccccccc ctctctacct tctctagatc ggcgttccgg
     1921 tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt
     1981 gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg
     2041 attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca
     2101 gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc
     2161 ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg
     2221 tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt
     2281 ctgtttcaaa ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata
     2341 ttcatagtta cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt
     2401 tgatgcgggt tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat
     2461 gtggtgtggt tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact
     2521 acctggtgta tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg
     2581 agtttaagat ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac
     2641 tgatgcatat acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct
     2701 atctattata ataaacaagt atgttttata attattttga tcttgatata cttggatgat
     2761 ggcatatgca gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg
     2821 cttggtactg tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcagggc
     2881 gatctattcg aatggacaag aagtactcga tcggcctcgc catcgggacg aactcagttg
     2941 gctgggccgt gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga
     3001 acaccgaccg ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg
     3061 agaccgctga ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga
     3121 acaggatctg ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat
     3181 tcttccaccg cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc
     3241 ccatcttcgg caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc
     3301 atctgcggaa gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg
     3361 ctctggccca catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag
     3421 acaacagcga tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg
     3481 aggagaaccc gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct
     3541 cgaagtccag gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc
     3601 tgttcgggaa cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg
     3661 atctcgctga ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata
     3721 acctcctggc ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt
     3781 cggacgccat cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac
     3841 tctctgcctc catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg
     3901 cgctggtccg ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga
     3961 acggctacgc tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca
     4021 agccaatcct ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg
     4081 acctcctgcg gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg
     4141 gggagctgca cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc
     4201 gggagaagat cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc
     4261 gggggaactc aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga
     4321 acttcgagga ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca
     4381 acttcgacaa gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt
     4441 acttcaccgt ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc
     4501 cagcgttcct gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc
     4561 ggaaggtcac ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt
     4621 cggtcgagat cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc
     4681 tcctgaagat catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg
     4741 aggacatcgt gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga
     4801 agacgtacgc ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca
     4861 ccggctgggg gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga
     4921 agaccatcct cgacttcctc aagagcgatg gcttcgccaa caggaacttc atgcaactga
     4981 tccacgatga cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg
     5041 gggactcgct gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca
     5101 tcctccagac cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg
     5161 agaacatcgt catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact
     5221 ctagggagcg catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca
     5281 aggagcaccc agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc
     5341 agaacggcag ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg
     5401 acgtcgatgc tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc
     5461 tcaccaggtc ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta
     5521 agaagatgaa gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt
     5581 tcgacaacct caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca
     5641 tcaagcggca gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact
     5701 ctcggatgaa cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca
     5761 ccctgaagtc taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg
     5821 agatcaacaa ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc
     5881 tgatcaagaa gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg
     5941 atgtgcgcaa gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact
     6001 tcttctactc aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga
     6061 tccggaagag accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg
     6121 gcagggattt cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga
     6181 agaccgaggt ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg
     6241 acaagctcat cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc
     6301 ctaccgtggc ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc
     6361 tcaagagcgt gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga
     6421 acccaatcga cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca
     6481 agctcccgaa gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg
     6541 ctggcgagct ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct
     6601 acctggcttc ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc
     6661 tgttcgtcga gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca
     6721 gcaagcgcgt gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc
     6781 accgggacaa gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga
     6841 acctcggcgc tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca
     6901 cctccacgaa ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg
     6961 agacgaggat cgacctctca caactcggcg gggataagag acccgcagca accaagaagg
     7021 cagggcaagc aaagaagaag aagggatccg gacgggctga cgcattggac gattttgatc
     7081 tggatatgct gggaagtgac gccctcgatg attttgacct tgacatgctt ggttcggatg
     7141 cccttgatga ctttgacctc gacatgctcg gcagtgacgc ccttgatgat ttcgacctgg
     7201 acatgctgat taactctaga agttccggat ctccgaaaaa gaaacgcaaa gttggtagcc
     7261 agtacctgcc cgacaccgac gaccggcacc ggatcgagga aaagcggaag cggacctacg
     7321 agacattcaa gagcatcatg aagaagtccc ccttcagcgg ccccaccgac cctagacctc
     7381 cacctagaag aatcgccgtg cccagcagat ccagcgccag cgtgccaaaa cctgcccccc
     7441 agccttaccc cttcaccagc agcctgagca ccatcaacta cgacgagttc cctaccatgg
     7501 tgttccccag cggccagatc tctcaggcct ctgctctggc tccagcccct cctcaggtgc
     7561 tgcctcaggc tcctgctcct gcaccagctc cagccatggt gtctgcactg gctcaggcac
     7621 cagcacccgt gcctgtgctg gctcctggac ctccacaggc tgtggctcca ccagccccta
     7681 aacctacaca ggccggcgag ggcacactgt ctgaagctct gctgcagctg cagttcgacg
     7741 acgaggatct gggagccctg ctgggaaaca gcaccgatcc tgccgtgttc accgacctgg
     7801 ccagcgtgga caacagcgag ttccagcagc tgctgaacca gggcatccct gtggcccctc
     7861 acaccaccga gcccatgctg atggaatacc ccgaggccat cacccggctc gtgacaggcg
     7921 ctcagaggcc tcctgatcca gctcctgccc ctctgggagc accaggcctg cctaatggac
     7981 tgctgtctgg cgacgaggac ttcagctcta tcgccgacat ggacttctcc gcactgctgg
     8041 gtagcggatc gggatctcgg gattccaggg aagggatgtt tttgccgaag cctgaggccg
     8101 gctccgctat tagtgacgtg tttgagggcc gcgaggtgtg ccagccaaaa cgaatccggc
     8161 catttcatcc tccaggaagt ccatgggcca accgcccact ccccgccagc ctcgcaccaa
     8221 caccaaccgg tccagtacat gagccagtcg ggtcactgac cccggcacca gtccctcagc
     8281 cactggatcc agcgcccgca gtgactcccg aggccagtca cctgttggag gatcccgatg
     8341 aagagactag ccaggctgtc aaagcccttc gggagatggc cgatactgtg attccccaga
     8401 aggaagaggc tgcaatctgt ggccaaatgg acctttccca tccgccccca aggggccatc
     8461 tggatgagct gacaaccaca cttgagtcca tgaccgagga tctgaacctg gactcacccc
     8521 tgaccccgga attgaacgag attctggata ccttcctgaa cgacgagtgc ctcttgcatg
     8581 ccatgcatat cagcacagga ctgtccatct tcgacacatc tctgttctag atgacgcgta
     8641 ctcgagtata tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct
     8701 taagtttgtg tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta
     8761 aatgaatgta agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg
     8821 ttttgttgga tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga
     8881 atatgattaa agataaggag ctcgttaacc ggtgacggac gtgggcaggt gtgatgaaaa
     8941 gccttagggt caggtggcac ttttcgggga aatgtgcgat gaacccctat ttgtttattt
     9001 ttctaaatac attcaaatat gtatccgcta atgagacaat aaccctgata aatgcttcaa
     9061 ttatattgaa aaaggaagag tatgagtatc caacatttcc gtgtcgccct tattcccttt
     9121 tttgcggcct tctgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9181 gctgaagatc agttgggtgc ccgagtgggt tacattgaac tggatctcaa cagcggcaaa
     9241 atcttagaga gttttcgccc cgaagaacgc ttcccaatga tgagcacttt caaagttctg
     9301 ctatgtggcg cggtgttatc ccgtattgat gctgggcaag agcaactcgg tcgccgcata
     9361 cactattctc agaatgactt ggttgagtat tcaccagtca cagaaaagca tcttacggat
     9421 ggcatgacag taagagaatt atgtagtgct gccataacca tgagtgataa cactgcggcc
     9481 aacttacttc tgacaacgat tggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9541 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9601 gacgagcgtg acaccacgat gcctgtagca atggcaacaa ctctccgcaa actattaact
     9661 ggcgaactac ttactcttgc ttcacgccag caactcattg actggatgga ggcggacaaa
     9721 gttgcaggac cacttctgcg ctcggcactt ccggctggct ggtttattgc tgataaatct
     9781 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9841 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9901 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga cgaagggaaa
     9961 taaatagtag cccgcctgat gtgcgggctt ttttttggtg gtggttacca gtgtcgctta
    10021 gctaccgtag gtcctcttag gcggtccgat tagcgacata gtcatcaat
//
