LOCUS       sequence-181867-        7134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7134)
  TITLE     Chao Plasmids
REFERENCE   2  (bases 1 to 7134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TrkA-RFP
FEATURES             Location/Qualifiers
     source          1..7134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        113..416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        417..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     571..591
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3180..3857
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             3180..3857
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3310..3328)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3578..3597
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3772..3795
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3974..4095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4011..4030)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4065..4084
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4102..4557)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4239..4260)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4451..4470
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4584..4688
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4690..5047
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4690..4881
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4713..4733)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4898..5033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5082..5876
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5082..5876
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5136..5155)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5746..5765
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6064..6083)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6108..6155
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6484..7072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6973..6992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt
       61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      421 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      481 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      541 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      601 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc
      661 agatctcgag ctcaagcttc gaattcgcgc acgtgtcgcg cgaggccggg cggcggcagc
      721 taggagcgca cggacggctg cgcggcccga gcaaggcggg cgccgccgcg atgctgcgag
      781 gccagcggct cgggcagctg ggctggcatc gcccggccgc ggggctaggc agtctgatga
      841 cttcgttgat gctggcctgt gcctccgccg catcctgtcg cgaggtctgc tgtcccgtgg
      901 gcccctcggg gttgcgctgc accagggcag ggaccctgaa taccctccgc ggcctgcggg
      961 gcgccgggaa cctgacggag ctctatgtgg aaaaccagcg ggatctgcaa cgcctggagt
     1021 ttgaggacct gcagggcctg ggggagttga gaagcctaac catcgtgaag agtggcctcc
     1081 gctttgtggc ccctgatgcc ttccatttca cccctcggct cagtcacctg aatctctcct
     1141 ccaatgcttt ggagtccctc tcctggaaaa ctgtgcaggg cctctcccta caggacttga
     1201 ccctgtcagg gaacccactg cactgttcct gtgccctatt gtggctccag cgctgggagc
     1261 aggaggattt gtgtggtgtg tatacacaaa agcttcaggg ctctgggtct ggagaccagt
     1321 tcctcccact gggacacaac aacagttgtg gtgtaccctc agtgaagatc cagatgccca
     1381 atgactctgt ggaagtgggg gatgacgttt ttctgcagtg ccaggtggag gggcaggccc
     1441 tacagcaggc tgactggatc ctcacagagc tggaagggac agccaccatg aagaaatctg
     1501 gagatctgcc atccctgggg ctaactctgg tcaatgtcac cagtgatctc aacaagaaga
     1561 atgtgacgtg ctgggcagag aatgatgtgg gccgggctga ggtctctgtc caagtcagcg
     1621 tctccttccc agccagtgtg catctgggca aagccgtgga acagcatcac tggtgcattc
     1681 ccttctctgt ggacgggcag ccagcaccgt ccctgcgctg gttcttcaac ggctctgtgc
     1741 tcaatgagac cagcttcatc ttcactcagt tcttggagtc agcgctgacc aatgagacca
     1801 tgcggcatgg ctgccttcgc ctcaaccagc ccacgcatgt caacaacggg aactacaccc
     1861 tgctggctgc caacccctat ggccaggctg ctgcctccat catggctgcc tttatggaca
     1921 acccttttga gttcaaccct gaggacccca tccctgtctc cttctcgcca gtggacacta
     1981 acagcacatc aagagaccca gtggagaaga aggacgaaac accttttggg gtctctgtgg
     2041 ctgtgggcct ggccgtctcc gccgccctct tcctttctgc cctcctccta gtgctcaaca
     2101 aatgtggaca gaggagcaaa tttgggatca accgccctgc tgtgctggcg ccagaggatg
     2161 ggctggccat gtccctacac ttcatgacac tgggtggcag ttctctttcc cctactgagg
     2221 gcaaaggctc cggactccag ggccacatca tggagaaccc acagtacttc agtgatacct
     2281 gtgtccacca tatcaagcgc caggacatca ttctcaagtg ggagctaggg gagggagcct
     2341 ttggaaaggt ctttcttgct gagtgctaca accttctgaa tgatcaggac aagatgctgg
     2401 tggctgtcaa ggcactgaag gagacatctg agaatgctcg tcaggacttc catcgtgagg
     2461 cagagctgct caccatgcta cagcaccaac acatcgtacg cttctttgga gtctgcacgg
     2521 agggtggccc attgctcatg gtcttcgagt acatgcgcca tggggacctc aaccgtttcc
     2581 tccggtccca cggacctgat gcaaaactgc tggctggcgg cgaggatgtg gctcctggtc
     2641 ctttgggcct tgggcagctt ctggctgtgg ctagccaggt ggctgctggt atggtgtacc
     2701 tagccagcct gcactttgtg caccgggacc tggccacacg caactgtctg gtgggtcagg
     2761 gactagtggt caagattgga gacttcggca tgagcaggga catctacagc acagactact
     2821 accgtgtggg aggtcggacc atgctgccca tccgctggat gcctccagag agcatcctct
     2881 accgcaagtt cagcaccgag agtgatgtgt ggagcttcgg ggtggtgctc tgggagatct
     2941 tcacctatgg aaagcaaccc tggtaccagc tctccaacac tgaggcgatc gagtgcatca
     3001 cgcagggccg ggagctggag cggccgcgcg cctgccctcc tgatgtctac gccatcatgc
     3061 gcggctgctg gcagcgtgag ccgcaacagc gcctcagcat gaaggatgtg cacgcgcggc
     3121 tgcaggcctt ggcacaggcg ccaccgagtt acctggacgt tctgggaccg ggggatccga
     3181 tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt caaggtgcgc atggagggct
     3241 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     3301 cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc
     3361 tgtcccctca gttccagtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     3421 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3481 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     3541 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     3601 tgggctggga ggcctccacc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3661 tcaagatgag gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct
     3721 acatggccaa gaagcccgtg cagctgcccg gcgcctacaa gaccgacatc aagctggaca
     3781 tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc
     3841 actccaccgg cgcctaaggc gactctagat cataatcagc cataccacat ttgtagaggt
     3901 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3961 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4021 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4081 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4141 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4201 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4261 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4321 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4381 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4441 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4501 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     4561 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4621 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4681 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     4741 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     4801 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     4861 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4921 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4981 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5041 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
     5101 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5161 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5221 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5281 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5341 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5401 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5461 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5521 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5581 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     5641 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5701 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5761 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     5821 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
     5881 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     5941 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6001 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
     6061 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     6121 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
     6181 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
     6241 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
     6301 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
     6361 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6421 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     6481 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     6541 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     6601 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     6661 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     6721 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     6781 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     6841 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     6901 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     6961 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7021 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     7081 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtt
//
