LOCUS       sequence-181869-        2052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module B, Promoter: none, Gene: none, Terminator: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_B0000
FEATURES             Location/Qualifiers
     source          1..2052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        960..1064
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcgggaga gcgcccacct gccaggggac ggcgcgacta gttttacgta cgttaattaa
      901 cccggtgacg caggtgatga aaagccttag ggtcaggtgg cacttttcgg ggaaatgtgc
      961 gatgaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctaatgagac
     1021 aataaccctg ataaatgctt caattatatt gaaaaaggaa gagtatgagt atccaacatt
     1081 tccgtgtcgc ccttattccc ttttttgcgg ccttctgcct tcctgttttt gctcacccag
     1141 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcccgagtg ggttacattg
     1201 aactggatct caacagcggc aaaatcttag agagttttcg ccccgaagaa cgcttcccaa
     1261 tgatgagcac tttcaaagtt ctgctatgtg gcgcggtgtt atcccgtatt gatgctgggc
     1321 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tattcaccag
     1381 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgtagt gctgccataa
     1441 ccatgagtga taacactgcg gccaacttac ttctgacaac gattggagga ccgaaggagc
     1501 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1561 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1621 caactctccg caaactatta actggcgaac tacttactct tgcttcacgc cagcaactca
     1681 ttgactggat ggaggcggac aaagttgcag gaccacttct gcgctcggca cttccggctg
     1741 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1801 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1861 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1921 ggtaactgtc agacgaaggg aaataaatag tagcccgcct gatgtgcggg cttttttttg
     1981 gtggtggtta ccagtgtcgc ttagctaccg taggtcctct taggcggtcc gattagcgac
     2041 atagtcatca at
//
