LOCUS       sequence-181903-        1644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to fluorescent protein 
            Katushka2 in the pCS2+ expression vector. It has the SP6 RNA 
            polymerase site for in vitro transcription..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1644)
  TITLE     Dr. Philipp Keller Lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCS2+ H2B-Katushka2
FEATURES             Location/Qualifiers
     source          1..1644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..112
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     94..111
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             163..540
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1267..1293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1297..1323
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1327..1353
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1392..1526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1416..1435)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1470..1489
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaacc
       61 gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc
      121 tacttgttct ttttgcagga tcccatcgac ggtaccgcca ccatgccaga gccagcgaag
      181 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
      241 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
      301 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
      361 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
      421 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
      481 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
      541 gatccaccgg tcgccaccat ggtatccgtg ctcataaccg aaaatatgca catgaagctg
      601 tacatggaag gaacagtcaa cgatcaccat ttcaagtgta ccagtgaggg agagggcaaa
      661 ccctatgagg gaacccagac catgaagatc aaggccgttg aaggtggacc cttgcctttc
      721 gcctttgaca ttctggccac tagctttatg tacggaagta aaacctttat caatcacacg
      781 caaggcatcc ctgatttctt caagcagagc tttccagagg gcttcacctg ggagaggatt
      841 accacctacg aggacggagg cgttctgacg gccactcagg atacgtcctt gcagaacggt
      901 tgcctgattt acaatgttaa gatcaacggc gtcaactttc catccaacgg tccggtaatg
      961 cagaagaaga ctctgggctg ggaggccagc accgaaaccc tttaccccgc tgacagcgga
     1021 ctgcgcggtc acagccagat ggctctgaag ttggtgggtg gtggatacct gcattgcagc
     1081 ctcaagacca cctaccgctc caagaaaccc gccaaaaatc tcaagatgcc cggcttctac
     1141 tttgtagatc gacggttgga gcggatcaag gaggcggata aggagacgta tgttgagcag
     1201 catgagatgg cggtggcacg gtattgcgat ttgccgtcga aactgggtgg taccagcggc
     1261 accgtttacc catacgatgt tcctgactat gcgggctatc cctatgacgt cccggactat
     1321 gcaggatatc catatgacgt tccagattac gctggcggat agtctagaac tatagtgagt
     1381 cgtattacgt agatccagac atgataagat acattgatga gtttggacaa accacaacta
     1441 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     1501 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     1561 ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg cattgggccc
     1621 ggtacccagc ttttgttccc ttta
//
