LOCUS       sequence-181909-        1647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to fluorescent protein 
            mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA 
            polymerase site for in vitro transcription..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1647)
  TITLE     Dr. Philipp Keller Lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCS2+ H2B-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..1647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..112
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     94..111
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             163..540
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1270..1296
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1300..1326
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1330..1356
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1395..1529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1419..1438)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1473..1492
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaacc
       61 gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc
      121 tacttgttct ttttgcagga tcccatcgac ggtaccgcca ccatgccaga gccagcgaag
      181 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
      241 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
      301 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
      361 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
      421 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
      481 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
      541 gatccaccgg tcgccaccat ggtttcgaag ggagaggagc tgatcaaaga gaatatgcac
      601 atgaagctgt atatggaggg aaccgtcgac aaccaccact ttaagtgcac cagcgagggt
      661 gagggaaagc cctacgaggg cacacagacc atgcgtatta aagttgtcga gggaggtcca
      721 ctgccatttg cattcgatat cctggccacc agtttcctct atggcagcaa gacattcatc
      781 aatcatactc agggaatacc agatttcttc aagcaatcct tccctgaagg cttcacatgg
      841 gaacgcgtta ctacctatga ggacggcgga gttctgactg ccacccaaga tactagcctg
      901 caggatggtt gcttgattta caacgtgaag atacgcggcg tgaactttac ctcgaacggc
      961 ccggttatgc agaaaaagac gctcggatgg gaggccttta cggagacgct gtaccccgcc
     1021 gatggcggtc ttgaaggtcg caacgatatg gctctgaagc tggtgggcgg ttcgcacctt
     1081 atagccaatg ccaagactac gtaccgtagt aagaagccag cgaagaacct gaagatgccc
     1141 ggtgtgtact acgtggacta taggctggag cgcattaagg aagctaacaa cgaaacctac
     1201 gtcgagcagc acgaagtcgc cgtcgcccgc tactgtgatt tgcctagtaa gttgggtcat
     1261 aagctcaact acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
     1321 tatgcaggat atccatatga cgttccagat tacgctggcg gataatctag aactatagtg
     1381 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     1441 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1501 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1561 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgcggcgcca atgcattggg
     1621 cccggtaccc agcttttgtt cccttta
//
