LOCUS       sequence-181915-        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse CBP CH2 domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Park S, Martinez-Yamout MA, Dyson HJ, Wright PE
  TITLE     The CH2 domain of CBP/p300 is a novel zinc finger.
  JOURNAL   FEBS Lett. 2013 Aug 19;587(16):2506-11. doi: 
            10.1016/j.febslet.2013.06.051. Epub 2013 Jul 4.
  PUBMED    23831576
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.febslet.2013.06.051"; journalName: "FEBS Lett"; date: 
            "2013-08-19- 19"; volume: "587"; issue: "16"; pages: "2506-11"
COMMENT     Sequence Label: pET-CH2 (1192-1318)
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             496..513
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(566..584)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      580..627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      664..1043
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(751..770)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtggggcgg tacattccct ctagaataat tttgtttaac tttaagaagg agatatacat
       61 atgcctgtca tgcagtctct tggatattgc tgtggacgaa agtatgagtt ctccccacag
      121 actttgtgct gttacggaaa gcagctgtgt acaattcctc gtgatgcagc ctactacagc
      181 tatcagaata ggtatcattt ctgtgagaag tgtttcacag agatccaggg cgagaatgtg
      241 accctgggtg acgacccttc ccaacctcag acgacaattt ccaaggatca atttgaaaag
      301 aagaaaaatg ataccttaga tcctgaacct tttgttgact gcaaagagtg tggccggaag
      361 atgcatcaga tttgtgttct acactatgac atcatttggc cttcaggttt tgtgtgtgac
      421 aactgtttga agaaaactgg cagataatag gatccgaatt cgagctccgt cgacaagctt
      481 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      541 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      601 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      661 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      721 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      781 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      841 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      901 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      961 gtggactctt gttccaaact gtaacaacac tcaaccctat ctcggtctat tcttttgatt
     1021 tataaggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aac
//
