LOCUS       sequence-181917-        1430 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of His tagged mouse CBP HAT domain (1324-1700).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Park S, Stanfield RL, Martinez-Yamout MA, Dyson HJ, Wilson IA, 
            Wright PE
  TITLE     Role of the CBP catalytic core in intramolecular SUMOylation and 
            control of histone H3 acetylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114.
  PUBMED    28630323
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114."
COMMENT     Sequence Label: His6tev CBP HAT (1324-1700)
FEATURES             Location/Qualifiers
     source          1..1430
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             157..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      1129..1138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acatatgtcg tactaccatc accatcacca tcacgattac
      181 gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tgggatccaa attcagtgct
      241 aagaggctgc agaccacacg attgggaaac cacttagaag acagagtgaa taagtttttg
      301 cggcgccaga atcaccctga agctggggag gtttttgtca gagtggtggc cagctcagac
      361 aagactgtgg aggtcaagcc gggaatgaag tcaaggtttg tggattctgg agagatgtcg
      421 gaatctttcc catatcgtac caaagcactc tttgcttttg aggagatcga tggagtcgat
      481 gtgtgctttt ttgggatgca tgtgcaagaa tacggctctg attgcccccc accaaataca
      541 aggcgtgtat acatatctta tctggacagt attcatttct tccggccccg ctgcctccgg
      601 acagctgttt accatgagat cctcatcgga tatctcgagt atgtgaagaa attggggtat
      661 gtgacaggac atatttgggc ctgtccccca agtgaaggag atgactatat ctttcattgc
      721 cacccccctg accagaaaat ccccaaacca aaacgactac aggagtggta caagaagatg
      781 ctggacaagg cgtttgcaga gaggatcatt aacgactata aggacatctt caaacaagcg
      841 aacgaagaca ggctcacgag tgccaaggag ttgccctatt ttgaaggaga tttctggcct
      901 aatgtgttgg aagaaagcat taaggaacta gaacaagaag aagaagaaag gaaaaaagaa
      961 gagagtactg cagcgagtga gactcctgag ggcagtcagg gtgacagcaa aaatgcgaag
     1021 aaaaagaaca acaagaagac caacaaaaac aaaagcagca ttagccgcgc caacaagaag
     1081 aagcccagca tgcccaatgt ttccaacgac ctgtcgcaga agctgtatgc caccatggag
     1141 aagcacaagg aggtattctt tgtgattcat ctgcatgctg ggcctgttat cagcactcag
     1201 ccccccatcg tggaccctga tcctctgctt agctgtgacc tcatggatgg gcgagatgcc
     1261 ttcctcaccc tggccagaga caagcactgg gaattctctt ccttacgccg ctccaaatgg
     1321 tccactctgt gcatgctggt ggagctgcac acacagggct aataggtcga cgagctcact
     1381 agtgcggccg ctttcgaatc tagagcctgc agtctcgagc accaccacca
//
