LOCUS       sequence-181918-        2310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ 
            (1079-1751).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Park S, Stanfield RL, Martinez-Yamout MA, Dyson HJ, Wilson IA, 
            Wright PE
  TITLE     Role of the CBP catalytic core in intramolecular SUMOylation and 
            control of histone H3 acetylation.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114.
  PUBMED    28630323
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Jun 19. pii: 201703105. doi: 
            10.1073/pnas.1703105114."
COMMENT     Sequence Label: His6tev CBP BCHZ (1079-1751)
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             157..174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             196..216
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      1864..1873
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat acatatgtcg tactaccatc accatcacca tcacgattac
      181 gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tgggatcctc ccagccacgc
      241 aaaaaaatct ttaaacccga ggagctacgc caggcactta tgccaactct agaagcactc
      301 tatcgacagg acccagagtc tttgcctttt cgtcagcctg tagatcctca gctcctagga
      361 atcccagatt attttgatat agtgaagaat cctatggacc tttctaccat caaacgaaag
      421 ctggacacag ggcaatatca agaaccctgg cagtatgtgg atgatgtctg gcttatgttc
      481 aacaatgcgt ggctatataa tcgtaaaacg tcccgtgtat ataaattttg cagtaaactt
      541 gcagaggtct ttgaacaaga aattgaccct gtcatgcagt ctcttggata ttgctgtgga
      601 cgaaagtatg agttctcccc acagactttg tgctgttacg gaaagcagct gtgtacaatt
      661 cctcgtgatg cagcctacta cagctatcag aataggtatc atttctgtga gaagtgtttc
      721 acagagatcc agggcgagaa tgtgaccctg ggtgacgacc cttcccaacc tcagacgaca
      781 atttccaagg atcaatttga aaagaagaaa aatgatacct tagatcctga accttttgtt
      841 gactgcaaag agtgtggccg gaagatgcat cagatttgtg ttctacacta tgacatcatt
      901 tggccttcag gttttgtgtg tgacaactgt ttgaagaaaa ctggcagacc tcggaaagaa
      961 aacaaattca gtgctaagag gctgcagacc acacgattgg gaaaccactt agaagacaga
     1021 gtgaataagt ttttgcggcg ccagaatcac cctgaagctg gggaggtttt tgtcagagtg
     1081 gtggccagct cagacaagac tgtggaggtc aagccgggaa tgaagtcaag gtttgtggat
     1141 tctggagaga tgtcggaatc tttcccatat cgtaccaaag cactctttgc ttttgaggag
     1201 atcgatggag tcgatgtgtg cttttttggg atgcatgtgc aagaatacgg ctctgattgc
     1261 cccccaccaa atacaaggcg tgtatacata tcttatctgg acagtattca tttcttccgg
     1321 ccccgctgcc tccggacagc tgtttaccat gagatcctca tcggatatct cgagtatgtg
     1381 aagaaattgg ggtatgtgac aggacatatt tgggcctgtc ccccaagtga aggagatgac
     1441 tatatctttc attgccaccc ccctgaccag aaaatcccca aaccaaaacg actacaggag
     1501 tggtacaaga agatgctgga caaggcgttt gcagagagga tcattaacga ctataaggac
     1561 atcttcaaac aagcgaacga agacaggctc acgagtgcca aggagttgcc ctattttgaa
     1621 ggagatttct ggcctaatgt gttggaagaa agcattaagg aactagaaca agaagaagaa
     1681 gaaaggaaaa aagaagagag tactgcagcg agtgagactc ctgagggcag tcagggtgac
     1741 agcaaaaatg cgaagaaaaa gaacaacaag aagaccaaca aaaacaaaag cagcattagc
     1801 cgcgccaaca agaagaagcc cagcatgccc aatgtttcca acgacctgtc gcagaagctg
     1861 tatgccacca tggagaagca caaggaggta ttctttgtga ttcatctgca tgctgggcct
     1921 gttatcagca ctcagccccc catcgtggac cctgatcctc tgcttagctg tgacctcatg
     1981 gatgggcgag atgccttcct caccctggcc agagacaagc actgggaatt ctcttcctta
     2041 cgccgctcca aatggtccac tctgtgcatg ctggtggagc tgcacacaca gggccaggac
     2101 cgctttgttt atacctgcaa tgagtgcaaa caccatgtgg aaacacgctg gcactgcact
     2161 gtgtgtgagg actatgacct ttgtatcaat tgctacaaca caaagagcca cacccataag
     2221 atggtgaagt gggggctagg cctagattaa taggtcgacg agctcactag tcgcggccgc
     2281 tttcgaatct agagcctgca gtctcgagca
//
