LOCUS       sequence-181949-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  binary construct of cytosolic mCherry with sfGFP11 tag for plant 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Park E, Lee HY, Woo J, Choi D, Dinesh-Kumar SP
  TITLE     Spatiotemporal Monitoring of Pseudomonas syringae Effectors via Type
            III Secretion Using Split Fluorescent Protein Fragments.
  JOURNAL   Plant Cell. 2017 Jul;29(7):1571-1584. doi: 10.1105/tpc.17.00047. 
            Epub 2017 Jun 14.
  PUBMED    28619883
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00047"; journalName: "Plant Cell"; date: "2017-07"; 
            volume: "29"; issue: "7"; pages: "1571-1584"
COMMENT     Sequence Label: PEP109E
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      58..310
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    332..356
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1656..2285
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2719..3786
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3852..4046
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4282..4304)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4390..4530
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4445..4464
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4545..4562)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4716..5304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4796..4815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5391..6185)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6610..6634
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6712..6886
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6926..7951)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8019..8696)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             10128..10832
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     10538..10557
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             10842..10871
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(10890..10906)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10890..10906)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             10938..10985
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
       61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
      121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
      181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
      241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
      301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
      361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
      421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
      481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
      541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
      601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
      661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
      721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
      781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
      841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
      901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
      961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
     1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
     1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
     1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
     1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
     1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
     1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
     1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
     1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
     1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
     1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
     1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
     1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
     1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
     1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
     1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
     1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
     1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
     2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
     2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
     2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
     2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
     2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
     2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
     2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
     2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
     2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
     2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
     2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
     2701 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     2761 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     2821 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     2881 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     2941 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     3001 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     3061 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     3121 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     3181 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     3241 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     3301 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     3361 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     3421 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     3481 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     3541 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     3601 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     3661 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     3721 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     3781 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     3841 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     3901 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     3961 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     4021 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     4081 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     4141 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     4201 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     4261 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     4321 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     4381 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     4441 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     4501 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     4561 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4621 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     4741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     4801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     4861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     4921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     4981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     5161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5341 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
     5401 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
     5461 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
     5521 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
     5581 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
     5641 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
     5701 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
     5761 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
     5821 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
     5881 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
     5941 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
     6001 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
     6061 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     6121 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     6181 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     6241 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     6301 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     6361 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     6421 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     6481 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     6541 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     6601 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     6661 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     6721 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     6781 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     6841 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     6901 tcgacagatc cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc
     6961 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
     7021 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
     7081 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
     7141 gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc
     7201 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
     7261 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
     7321 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
     7381 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
     7441 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
     7501 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
     7561 tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa
     7621 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
     7681 tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg
     7741 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
     7801 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
     7861 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga
     7921 cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggga tctgcgaaag
     7981 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     8041 aatgaacttc cttatataga ggaaggtctt gcgaaggata gtgggattgt gcgtcatccc
     8101 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     8161 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     8221 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
     8281 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
     8341 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
     8401 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttatcacat caatccactt
     8461 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     8521 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     8581 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     8641 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     8701 ccctttggtc ttctgagact gtatctttga tttggagtag acgagagtgt cgtgctccac
     8761 catgttgggc ccggcgcgcc gaattcccgg ggatccgtcg aggatcagga tattcttgtt
     8821 taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg gataagttcc
     8881 cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt acattgttat
     8941 taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg gaccaggccc
     9001 caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc accaaaccac
     9061 ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat cctatgcaaa
     9121 tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg gataatttca
     9181 caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt tataagccca
     9241 cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc acgaagaatt
     9301 ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta ttgccaattt
     9361 tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat aaatcgtaac
     9421 gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg gttgtcgacg
     9481 agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa
     9541 ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt
     9601 gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt
     9661 agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc
     9721 caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct
     9781 ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt
     9841 cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat
     9901 cttagatcga agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag
     9961 ggtttcataa atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct
    10021 tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt
    10081 cgattaatct gagtttttct gattaacaga tgaagcttat gggtaccgtg agcaagggcg
    10141 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
    10201 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
    10261 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
    10321 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
    10381 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
    10441 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
    10501 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
    10561 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
    10621 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
    10681 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
    10741 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
    10801 actccaccgg cggcatggac gagctgtaca aggcatgcat ggagcaaaag ctcatttctg
    10861 aagaggactt gaattcctgc agcccggggg atccactagt tctagagctc ggcgacggcg
    10921 gcagcggcgg cggcagccgg gaccacatgg tgctgcacga gtacgtgaac gccgccggca
    10981 tcacatgaag atctatctaa acg
//
