LOCUS       sequence-181977-        2803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of fission yeast (S. pombe) Tropomyosin, cdc8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Skoumpla K, Coulton AT, Lehman W, Geeves MA, Mulvihill DP
  TITLE     Acetylation regulates tropomyosin function in the fission yeast 
            Schizosaccharomyces pombe.
  JOURNAL   J Cell Sci. 2007 May 1;120(Pt 9):1635-45.
  PUBMED    17452625
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2007-05-1"; volume: "120(Pt 9)"; pages: "1635-45"
COMMENT     Sequence Label: pJC20cdc8
FEATURES             Location/Qualifiers
     source          1..2803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     75..94
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             125..147
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(707..725)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      721..768
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(812..830)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        898..1002
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1003..1071,1072..1863)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1221..1240)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2034..2622
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2776..2793
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaggatct
       61 cgatcccgcg aaattaatac gactcactat agggagacca caacggtttc cctctagaaa
      121 taattttgtt taactttaag aaggagatgt acatatggat aagcttagag agaaaattaa
      181 tgccgctcgt gctgagactg atgaggctgt cgctcgtgct gaagcagctg aagccaagtt
      241 gaaagaagtt gaacttcaac tttctcttaa agagcaagaa tatgagtctc tttcgaggaa
      301 aagcgaagcc gccgagtctc aactcgaaga gttagaagaa gaaactaagc agttacgtct
      361 caaagccgac aatgaagata ttcaaaagac ggaagccgag cagttgagcc gtaaagttga
      421 gcttttagag gaagaactag aaactaatga caagctactt cgcgaaacca ccgaaaagat
      481 gcgtcaaact gatgttaagg ctgagcattt tgaacgtcgc gtccaatctc tagagcgtga
      541 gcgtgatgat atggaacaga aactagaaga aatgactgac aagtatacta aggtcaaggc
      601 tgaattagat gaagttcacc aagctcttga ggatttgtag ggattcccct agctaactga
      661 tccggctgct aacaaagccc aaaggaagct gagttggctg ctgccaccgc tgagcaataa
      721 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
      781 actatagata attcttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
      841 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
      901 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      961 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     1021 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1081 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     1141 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     1201 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     1261 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     1321 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataagc
     1381 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     1441 accgcttttt ttcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     1501 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     1561 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     1621 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     1681 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     1741 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg cagtcaggca
     1801 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     1861 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     1921 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1981 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2161 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2221 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2281 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2341 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2401 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     2461 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2521 ggggggaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2581 cgatttttgt gatgctcgtc aggggggccg agcctatgga aaaacgccag caacgcggcc
     2641 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2701 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     2761 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
