LOCUS       sequence-181988-        6959 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term of HCFC1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Wilson AC, Boutros M, Johnson KM, Herr W
  TITLE     HCF-1 amino- and carboxy-terminal subunit association through two 
            separate sets of interaction modules: involvement of fibronectin 
            type 3 repeats.
  JOURNAL   Mol Cell Biol. 2000 Sep;20(18):6721-30.
  PUBMED    10958670
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2000-09"; volume: "20(18)"; pages: "6721-30"
COMMENT     Sequence Label: pCGT-HCF-1-C600
FEATURES             Location/Qualifiers
     source          1..6959
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..507
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     458..478
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     504..528
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           597..636
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          2648..3220
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3352..3371)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3417..3472
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3471..3490)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        complement(3809..4005)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3823..3958
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4072..4089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4243..4831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4323..4342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5002..5793,5794..5862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5625..5644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5863..5967)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6035..6053
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6091..6113)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6213..6232
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6299..6754)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6436..6457)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6648..6667
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6884..6906
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6898..6915
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6899..6915
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6922..6941
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6922..6940
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        join(6959,1..303)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
       61 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      121 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      181 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      241 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      301 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      361 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      421 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      481 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      541 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgggggatc
      601 ttggtggcgt gaaactcccg cacctcttcg gccagcgcct tgtagaagcg cgtatggctt
      661 ctaggatggc atcgatgaca ggtggccaac agatgggttc taggatggca tcgatgacag
      721 gtggccaaca gatgggttct agaaccagta gatctgtcac ttccaacatg agttcaaacc
      781 aagacccccc acctgctgcc agcgatcagg gagaggtgga gagcacccag ggcgacagcg
      841 tgaacatcac cagctccagt gccatcacga caaccgtgtc ctccacactg acgcgggctg
      901 tgaccaccgt gacgcagtcc acaccggtcc cgggcccctc tgtgccgccc ccagaggaac
      961 tccaggtgtc gccaggtcct cgccagcagc tgccgccacg gcagcttctg cagtcggctt
     1021 ccacagccct gatgggggag tccgccgagg tcctgtcagc ctcccagacc cctgagctcc
     1081 cggccgccgt ggatctgagc agcacagggg agccatcttc gggccaggag tctgccggct
     1141 ctgcggtggt ggccactgtg gtggtccagc cacccccacc cacacagtcc gaagtagacc
     1201 agttatcact tccccaagag ctaatggccg aggcccaagc tggcaccacc accctcatgg
     1261 taacggggct cacccccgag gagctggcag tgacggctgc tgcagaagca gctgcccagg
     1321 ccgcagccac ggaggaagcc caggccctgg ccatccaggc ggtgctccag gccgcgcagc
     1381 aggccgtcat gggcaccggc gagcccatgg acacctccga ggcagcagca accgtgactc
     1441 aggcggagct ggggcacctg tcggccgagg gtcaggaggg ccaggccacc accataccca
     1501 ttgtgctgac acagcaggag ctggctgccc tggtgcagca gcagcagctg caggaggccc
     1561 aggcccagca gcagcatcac cacctcccca ctgaggccct ggcccctgcc gacagtctca
     1621 acgacccagc cattgagagc aattgcctca atgagctggc cggcacggtc cccagcactg
     1681 tggcgctgct gccctcaacg gccactgaga gcctggctcc atccaacaca tttgtggccc
     1741 cccagccggt tgtggtggcc agcccagcca agctgcaggc tgcagctacc ctgaccgaag
     1801 tggccaatgg catcgagtcc ctgggtgtga agccagacct gccgccccca cccagcaaag
     1861 cccccatgaa gaaggaaaac cagtggtttg atgtgggagt cattaagggc accaatgtaa
     1921 tggtgacaca ctatttcctg ccaccagatg atgctgtccc atcagacgat gatttgggca
     1981 ccgtccctga ctataaccag ctgaagaagc aggagctgca gccaggcaca gcctataagt
     2041 ttcgtgttgc cggaatcaat gcctgtggcc gggggccctt cagcgaaatc tcagccttta
     2101 agacgtgcct gcctggtttc ccaggggccc cttgtgccat taaaatcagc aaaagtccgg
     2161 atggtgctca cctcacctgg gagccaccct ctgtgacctc cggcaagatt atcgagtact
     2221 ccgtgtacct ggccatccag agctcacagg ctgggggcga gctcaagagc tccaccccgg
     2281 cccagctggc cttcatgcgg gtgtactgtg ggcccagccc ctcctgcctg gtgcagtcct
     2341 ccagcctttc caacgcccac atcgactaca ccaccaagcc cgccatcatc ttccgcatcg
     2401 ccgcccgcaa tgagaagggc tatggcccgg ccacacaagt gaggtggctg caggaaacca
     2461 gtaaagacag ctctggcacc aagccagcca acaagcggcc catgtcctct ccagaaatga
     2521 aatctgctcc aaagaaatct aaggccgatg gtcagggcga gcagaagctg atctctgagg
     2581 aggacacggt atcgcgataa gcttgatatc gaattcctgc agcccggggg atcctgagaa
     2641 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     2701 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     2761 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     2821 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     2881 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     2941 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     3001 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     3061 aaatattctt attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg
     3121 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     3181 ctctgctaac catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt
     3241 tgttgtgctg tctcatcatt ttggcaaaga attcactcct caggtgcagg ctgcctatca
     3301 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     3361 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     3421 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     3481 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     3541 aacatatgcc atatgctggc tgccatgaac aaaggtggct ataaagaggt catcagtata
     3601 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     3661 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     3721 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     3781 ttctcttatg aagatcctcg agaagctgtt tgcaaaagcc taggcctcca aaaaagcctc
     3841 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     3901 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     3961 gcggagttag gggcgggact atggttgctg actaattgag atgcagctgc attaatgaat
     4021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4681 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     5821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6001 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6061 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6121 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6181 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6241 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa
     6301 attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     6361 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     6421 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     6481 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     6541 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     6601 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     6661 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     6721 cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt caggctgcgc
     6781 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     6841 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     6901 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcgcccc
//
