LOCUS       sequence-182045-        2119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: none, Gene: none (BaeI site for GT donor 
            cloning), Terminator: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C0000
FEATURES             Location/Qualifiers
     source          1..2119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1027..1131
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgagctttgg gtacgtcacg tggctcgagc
      901 gcgtagtcct cggtaatatc gcagaacaaa agtacctgat atcgagtgta cttcaagtca
      961 caccggcgag tgactagcag gtgatgaaaa gccttagggt caggtggcac ttttcgggga
     1021 aatgtgcgat gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgcta
     1081 atgagacaat aaccctgata aatgcttcaa ttatattgaa aaaggaagag tatgagtatc
     1141 caacatttcc gtgtcgccct tattcccttt tttgcggcct tctgccttcc tgtttttgct
     1201 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc ccgagtgggt
     1261 tacattgaac tggatctcaa cagcggcaaa atcttagaga gttttcgccc cgaagaacgc
     1321 ttcccaatga tgagcacttt caaagttctg ctatgtggcg cggtgttatc ccgtattgat
     1381 gctgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
     1441 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgtagtgct
     1501 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
     1561 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1621 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     1681 atggcaacaa ctctccgcaa actattaact ggcgaactac ttactcttgc ttcacgccag
     1741 caactcattg actggatgga ggcggacaaa gttgcaggac cacttctgcg ctcggcactt
     1801 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1861 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1921 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1981 aagcattggt aactgtcaga cgaagggaaa taaatagtag cccgcctgat gtgcgggctt
     2041 ttttttggtg gtggttacca gtgtcgctta gctaccgtag gtcctcttag gcggtccgat
     2101 tagcgacata gtcatcaat
//
