LOCUS       sequence-182056-        1718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to fluorescent protein 
            mEmerald in the pCS2+ expression vector. It has the SP6 RNA 
            polymerase site for in vitro transcription..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1718)
  TITLE     Dr. Philipp Keller Lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCS2+ H2B-mEmerald
FEATURES             Location/Qualifiers
     source          1..1718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        66..84
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     66..83
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             135..512
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      525..534
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(531..533,534..536,537..1250)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(576..597)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(837..856)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1184..1205
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1298..1432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1322..1341)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1376..1395
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1545..1565)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1545..1563)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1584..1600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1584..1600)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1597..1619)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1608..1624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1632..1638,1639..1656,1657..1662))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1677..1698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggtctatata agcaatgctc gtttagggaa ccgccattct gcctggggac gtcggagcaa
       61 gcttgattta ggtgacacta tagaatacaa gctacttgtt ctttttgcag gatcccatcg
      121 acggtaccgc caccatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
      181 agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg
      241 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
      301 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
      361 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
      421 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
      481 ctaaggccat caccaagtac accagcgcta aggatccacc ggtcgccacc atggtgagca
      541 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      601 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
      661 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
      721 ccttgaccta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag cagcacgact
      781 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
      841 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
      901 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
      961 acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1021 tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     1081 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     1141 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1201 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa agcggcccct
     1261 ctcgagcctc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     1321 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     1381 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     1441 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     1501 ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta
     1561 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1621 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1681 gtgagctaac tcacattaat tgcgttgcgc tcactgcc
//
