LOCUS       sequence-182058-        3511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C2806
FEATURES             Location/Qualifiers
     source          1..3511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2419..2523
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccagct ggcttgtggg
      901 gaccagacaa aaaaggaatg gtgcagaatt gttaggcgca cctaccaaaa gcatctttgc
      961 ctttattgca aagataaagc agattcctct agtacaagtg gggaacaaaa taacgtggaa
     1021 aagagctgtc ctgacagccc actcactaat gcgtatgacg aacgcagtga cgaccacaaa
     1081 acagctggct tgtggggacc agacaaaaaa ggaatggtgc agaattgtta ggcgcaccta
     1141 ccaaaagcat ctttgccttt attgcaaaga taaagcagat tcctctagta caagtgggga
     1201 acaaaataac gtggaaaaga gctgtcctga cagcccactc actaatgcgt atgacgaacg
     1261 cagtgacgac cacaaaagaa ttccctctat ataagaaggc attcattccc atttgaagga
     1321 tcatcagata ctgaaccaat atttctcact ctaagaaatt aagagctttg tattcttcaa
     1381 tgagaggcta agacctgcag gaaaaatgga tcattatctt gatattagac ttagacctga
     1441 tccagaattt ccaccagctc aacttatgtc tgttcttttt ggaaaacttc atcaagctct
     1501 tgttgctcaa ggaggagata gaattggagt ttcttttcct gatcttgatg aatcaagatc
     1561 aagacttgga gaaagactta gaattcatgc ttctgctgat gatcttagag ctttgcttgc
     1621 tagaccttgg cttgaaggac ttagagatca tcttcaattt ggagaaccag ctgttgttcc
     1681 acatccaact ccttatagac aagtttcaag agttcaagct aaatctaatc cagaaagact
     1741 tagaagaaga cttatgagaa gacatgatct ttctgaagaa gaagctagaa aaagaattcc
     1801 tgatactgtt gctagagctt tggatttgcc ttttgttaca cttagatcac aatctactgg
     1861 acaacatttt agacttttta ttagacatgg accacttcaa gttactgctg aagaaggagg
     1921 atttacttgt tatggacttt ctaagggagg ttttgttcct tggttttgat aactcgaggt
     1981 tcgagtatta tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt
     2041 actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag
     2101 ttaatgaatg atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt
     2161 atttgttgtg tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa
     2221 aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag
     2281 ttgtgagctc gcgcgtagtc ctcggtaata tcgcagaaca aaagtacctg atatcgagtg
     2341 tacttcaagt cacaccggcg agtgactagc aggtgatgaa aagccttagg gtcaggtggc
     2401 acttttcggg gaaatgtgcg atgaacccct atttgtttat ttttctaaat acattcaaat
     2461 atgtatccgc taatgagaca ataaccctga taaatgcttc aattatattg aaaaaggaag
     2521 agtatgagta tccaacattt ccgtgtcgcc cttattccct tttttgcggc cttctgcctt
     2581 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2641 gcccgagtgg gttacattga actggatctc aacagcggca aaatcttaga gagttttcgc
     2701 cccgaagaac gcttcccaat gatgagcact ttcaaagttc tgctatgtgg cgcggtgtta
     2761 tcccgtattg atgctgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2821 ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2881 ttatgtagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2941 attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3001 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3061 atgcctgtag caatggcaac aactctccgc aaactattaa ctggcgaact acttactctt
     3121 gcttcacgcc agcaactcat tgactggatg gaggcggaca aagttgcagg accacttctg
     3181 cgctcggcac ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3241 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3301 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3361 gcctcactga ttaagcattg gtaactgtca gacgaaggga aataaatagt agcccgcctg
     3421 atgtgcgggc ttttttttgg tggtggttac cagtgtcgct tagctaccgt aggtcctctt
     3481 aggcggtccg attagcgaca tagtcatcaa t
//
