LOCUS       sequence-182059-        4867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor 
            cloning), Terminator: pea rbcsE9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C2813
FEATURES             Location/Qualifiers
     source          1..4867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        3775..3879
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccgaag ccaactaaac
      901 aagaccataa ccatggtgac atttgacata gttgtttact acttgcttga gccccaccct
      961 tgcttatcgg ttgaacatta caagatacac tgcgggtggc ctaaggcaca ccgtccgaaa
     1021 ccggcaaacc aagcctgatc gccgaaatcc aaaatcacta ccggcaatct ctaaagttta
     1081 tttcatcctt atatgacgag gaaagaaaag aagagagaaa taatatctta acttctaaat
     1141 cagtcgcgtc aactttctcg gctaagaaag tgagcactat catttcgaag accatgtcat
     1201 gagtgccgac ttgccatatc ttattatatt cttatttatt taattataat cccattgcaa
     1261 tacgtctatt ctatcatggc ctgccactaa cgctccgtct aacgtcgtta agccattgtc
     1321 ataagcggct gctcaaaact cttcccggtg gaggcgaggc gttaacggcg tctacaaatc
     1381 taacggccac caaccatcca gccgcctctc gaaagctccg ctccgatcgc ggaaattgcg
     1441 tggcggagac gagcgggctc ctctcacacg gcccggaacc gtcacggcac gggtggggga
     1501 ttccttcccc aaccctcccc acctctcctc cccccgtcgc agcccataaa tacagggccc
     1561 tccgcgcctc ttcccacaat ctcacatcgt ctcatcgttc ggagcgcaca acccccgggt
     1621 tccaaatcca aattgctctc ctcgcgaccc tcggcgatcc ttcccccgct tcaaggtacg
     1681 gcgatcgtct cccccgtcct cttgccccat ctcctcgctc ggcgtggttt ggtggttctg
     1741 cttggtctgt ggctaggaac taggctgagg cgttgacgaa atcatgctag atccgcgtgt
     1801 ttcctgatcg tgggtggctg ggaggtgggg ttttcgtgta gatctgatcg gttccgctgt
     1861 ttatcctgtc atgctcatgt gatttgtggg gattttaggt cgtttgtccg ggaatcgtgg
     1921 ggttgcttct aggctgttcg tagatgagat cgttctcacg atctgctggg tcgctgccta
     1981 ggttcagcta ggtctgccct gtttttgggt tcgttttcgg gatctgtacg tgcatctatt
     2041 atctggttcg atggtgctag ctaggaacaa acaactgatt cgtccgatcg attgttttgt
     2101 tgccatgtgc aaggttaggt cgttatctga ttgctgtaga tcagagtaga ataagatcat
     2161 cacaagctag ctcttgggct tattatgaat ctgcgtttgt tgcatgatta agatgattat
     2221 gctttttctt atgctgccgt ttgtatatga tgcggtagct tttaactgaa tagcacacct
     2281 ttcctgttta gttagattag attagattgc atgatagatg aggatatatg ctgctacatc
     2341 agtttgatga ttctctggta cctcataatc aactagctca tgtgcttaaa ttgaaactgc
     2401 atgtgccaca tgattaagat gctaagattg gtgaagatat atacgctgct gttcctatag
     2461 gatcctgtag cttttacctg gtcaacatgc atcgtcctgt tatggataga tatgcatgat
     2521 agatgaagat atgtactgct acaatttgat gattcttttg tgcacctgat gatcatgcat
     2581 gctctttgcc cttactttga tatacttgga tgatggcatg cttagtacta atgatgtgat
     2641 gaacacacat gacctgttgg tatgaatatg atgttgctgt ttgcttgtga tgagttctgt
     2701 ttgtttactg ctaggcactt accctgttgt ctggttctct tttgcagaac ctgcaggaag
     2761 catggaccac tacctcgaca tcaggctcag gccagaccca gagttcccac cagcccagct
     2821 catgtccgtc ctcttcggca agctccacca ggccctcgtg gcccagggcg gcgacaggat
     2881 cggcgtgtcc ttcccagacc tcgacgagtc caggtccagg ctcggcgaga ggctccgcat
     2941 ccacgcctcc gccgacgacc tcagggccct cctcgccagg ccgtggctgg agggcctcag
     3001 ggaccacctc cagttcggcg agccagccgt ggtgccacac ccaaccccat acaggcaagt
     3061 gtccagggtg caagccaagt ccaacccaga gaggctcagg aggaggctca tgaggaggca
     3121 cgacctctcc gaggaagagg ccaggaagcg catcccagac accgtggcca gggccctcga
     3181 cctcccattc gtgaccctca ggtcccagtc caccggccag cacttccgcc tcttcatcag
     3241 gcacggccca ctccaggtga ccgccgagga gggcggcttt acctgctacg gcctctccaa
     3301 gggcggcttc gtgccgtggt tctgataact cgaggttcga gtattatggc attgggaaaa
     3361 ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat tcggttttcg
     3421 ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat ggtccttttg
     3481 ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt gaatttgaaa
     3541 ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg tggcctctaa
     3601 tgaccgaagt taatatgagg agtaaaacac ttgtagttgt gagctcgcgc gtagtcctcg
     3661 gtaatatcgc agaacaaaag tacctgatat cgagtgtact tcaagtcaca ccggcgagtg
     3721 actagcaggt gatgaaaagc cttagggtca ggtggcactt ttcggggaaa tgtgcgatga
     3781 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctaat gagacaataa
     3841 ccctgataaa tgcttcaatt atattgaaaa aggaagagta tgagtatcca acatttccgt
     3901 gtcgccctta ttcccttttt tgcggccttc tgccttcctg tttttgctca cccagaaacg
     3961 ctggtgaaag taaaagatgc tgaagatcag ttgggtgccc gagtgggtta cattgaactg
     4021 gatctcaaca gcggcaaaat cttagagagt tttcgccccg aagaacgctt cccaatgatg
     4081 agcactttca aagttctgct atgtggcgcg gtgttatccc gtattgatgc tgggcaagag
     4141 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc accagtcaca
     4201 gaaaagcatc ttacggatgg catgacagta agagaattat gtagtgctgc cataaccatg
     4261 agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa ggagctaacc
     4321 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4381 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaact
     4441 ctccgcaaac tattaactgg cgaactactt actcttgctt cacgccagca actcattgac
     4501 tggatggagg cggacaaagt tgcaggacca cttctgcgct cggcacttcc ggctggctgg
     4561 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4621 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4681 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4741 ctgtcagacg aagggaaata aatagtagcc cgcctgatgt gcgggctttt ttttggtggt
     4801 ggttaccagt gtcgcttagc taccgtaggt cctcttaggc ggtccgatta gcgacatagt
     4861 catcaat
//
