LOCUS       sequence-182062-        8379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human PRDX5-E80K in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8379)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 8379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pLD-puro-Cc-PRDX5E80K-VA
FEATURES             Location/Qualifiers
     source          1..8379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     510..534
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    565..589
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1235..1259)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1339..1362
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1399..1422
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             1435..1452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1462..1482
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1495..1515
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1528..1593
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1641..1758
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1807..2317
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2236..2254
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2339..2938
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2339..2358)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2835..2855
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3066..3299
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3371..3492
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3408..3427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3462..3481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3532..3667
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3687..3706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3688..3706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3716..3733)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3716..3732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3725..3747)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3874..4329
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3961..3980)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4171..4192
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4355..4459
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4460..4528,4529..5320)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4678..4697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5491..6079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5980..5999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6233..6250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6403..6408,6409..6426,6427..6433)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6441..6457
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6446..6468
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6465..6481
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6465..6481
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6500..6520
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6502..6520
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6548..6774
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6775..6955
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7002..7127
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7620..7853
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8038..8082
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      541 agacgccatc cacgctgtac gcgtacaagt ttgtacaaaa aagttggcac catgggacta
      601 gctggcgtgt gcgccctgag acgctcagcg ggctatatac tcgtcggtgg ggccggcggt
      661 cagtctgcgg cagcggcagc aagacggtgc agtgaaggag agtgggcgtc tggcggggtc
      721 cgcagtttca gcagagccgc tgcagccatg gccccaatca aggtgggaga tgccatccca
      781 gcagtggagg tgtttgaagg ggagccaggg aacaaggtga acctggcaaa gctgttcaag
      841 ggcaagaagg gtgtgctgtt tggagttcct ggggccttca cccctggatg ttccaagaca
      901 cacctgccag ggtttgtgga gcaggctgag gctctgaagg ccaagggagt ccaggtggtg
      961 gcctgtctga gtgttaatga tgcctttgtg actggcgagt ggggccgagc ccacaaggcg
     1021 gaaggcaagg ttcggctcct ggctgatccc actggggcct ttgggaagga gacagactta
     1081 ttactagatg attcgctggt gtccatcttt gggaatcgac gtctcaagag gttctccatg
     1141 gtggtacagg atggcatagt gaaggccctg aatgtggaac cagatggcac aggcctcacc
     1201 tgcagcctgg cacccaatat catctcacag ctcttgccaa ctttcttgta caaagtggtt
     1261 gattctagag cgatcgccag agagctgttc aacctgctgg gcgagaacca gccccccgtg
     1321 gtgatcaagg cgatcgcctg gagccaccct cagttcgaga agggaggagg aagcggcgga
     1381 ggcagcggag gaggaagctg gagccacccg cagttcgaga aaggcggaag cggccatcac
     1441 catcaccatc accctgcagg agaaaatctg tacttccagg ggagaggagg cagcgagaac
     1501 ctgtactttc aggggcctgc aggaagagac tacaaagacc atgacggtga ttataaagat
     1561 catgacatcg attacaagga tgacgatgac aagtagttcg aattctcgac ctcgagacaa
     1621 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     1681 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     1741 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggccgcgg
     1801 ctcgaggggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg
     1861 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt
     1921 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac
     1981 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg
     2041 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat
     2101 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga
     2161 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct
     2221 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg
     2281 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat
     2341 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     2401 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     2461 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     2521 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     2581 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     2641 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     2701 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     2761 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     2821 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     2881 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     2941 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc
     3001 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     3061 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     3121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     3181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     3241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     3301 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     3361 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     3421 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     3481 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     3541 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     3601 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     3661 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     3721 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3781 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3841 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     3901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3961 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4021 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4081 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4141 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4201 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4261 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     4321 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4381 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4441 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4501 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4561 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4621 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4681 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4741 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4801 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4861 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4921 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4981 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5041 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5101 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5161 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5221 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5281 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5341 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5401 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5461 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5521 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5581 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5641 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5701 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5761 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5821 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5881 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5941 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6001 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6061 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6121 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6181 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6241 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6301 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6361 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6421 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6481 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     6541 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     6601 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     6661 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     6721 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     6781 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     6841 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     6901 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     6961 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     7021 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     7081 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     7141 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     7201 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     7261 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     7321 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     7381 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     7441 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     7501 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     7561 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     7621 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     7681 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7741 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     7801 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     7861 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     7921 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     7981 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     8041 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     8101 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     8161 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     8221 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     8281 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     8341 cgatcacgag actagcctcg agcggccgcc cccttcacc
//
