LOCUS       sequence-182063-        6661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: FMV 34S, Gene: TALE-VPR backbone with Esp3I ccdb
            cassette for repeat cloning, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C3106
FEATURES             Location/Qualifiers
     source          1..6661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             1418..1483
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1490..1510
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2258..2563)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2360..2379)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             3547..3696
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3721..3741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4171..4527
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4546..5115
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     promoter        5569..5673
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gcaacgccgg cgtaatatgg cgcgccagct ggcttgtggg
      901 gaccagacaa aaaaggaatg gtgcagaatt gttaggcgca cctaccaaaa gcatctttgc
      961 ctttattgca aagataaagc agattcctct agtacaagtg gggaacaaaa taacgtggaa
     1021 aagagctgtc ctgacagccc actcactaat gcgtatgacg aacgcagtga cgaccacaaa
     1081 acagctggct tgtggggacc agacaaaaaa ggaatggtgc agaattgtta ggcgcaccta
     1141 ccaaaagcat ctttgccttt attgcaaaga taaagcagat tcctctagta caagtgggga
     1201 acaaaataac gtggaaaaga gctgtcctga cagcccactc actaatgcgt atgacgaacg
     1261 cagtgacgac cacaaaagaa ttccctctat ataagaaggc attcattccc atttgaagga
     1321 tcatcagata ctgaaccaat atttctcact ctaagaaatt aagagctttg tattcttcaa
     1381 tgagaggcta agacctgcag gaaaaatggc ttcctccgat tataaggatc acgatggcga
     1441 ctacaaggac cacgatattg actacaaaga cgatgacgat aaaatggctc ctaagaaaaa
     1501 gcgcaaagtc ggtatccatg gcgttcccgc tagcgtgtcc cggacccggc tgccatctcc
     1561 ccctgcgccc tctcctgcgt tctcggcggg cagcttcagc gatctgctcc gtccgttcga
     1621 tccgtcgctt cttgatacat cgcttcttga ttcgatgcct gccgtcggca cgccgcatac
     1681 agcggctgcc ccagcagagt gggatgaggc gcaatcggct ctgcgtgcag ccgatgaccc
     1741 gccacccacc gtgcgtgtcg ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc
     1801 gcgacggcgt gctgcgcaac cctccgacgc ttcgccggcc gcgcaggttg atctacgcac
     1861 gctcggctac agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc
     1921 gcagcaccac gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag
     1981 ccaacacccg gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt
     2041 gccagaggcg acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc
     2101 cctggaggcc ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac
     2161 aggccaactt gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc
     2221 cagcaggaat gcgttaacag gtgcccccct ggagacgtta tattccccag aacatcaggt
     2281 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
     2341 cggaaaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
     2401 gcaccaccgg gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg
     2461 ggatcaccat ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac
     2521 gataacggct ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc
     2581 tcgtcagcaa aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga
     2641 ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt
     2701 accagaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa
     2761 ctgtcactgt aatacgctgc ttcatagcat acctcctttt gacatacttc gggtatacat
     2821 atcagtatat attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc
     2881 ttcgtctcca acgaccacct cgtcgccttg gcctgcctcg gcggacgtcc tgccatggat
     2941 gcagtgaaaa agggattgcc gcacgcgccg gaattgatca gaagagtcaa tcgccgtatt
     3001 ggcgaacgca cgtcccatcg cgttgccgac tacgcgcaag tggttcgcgt gctggagttt
     3061 ttccagtgcc actcccaccc agcgtacgca tttgatgagg ccatgacgca gttcgggatg
     3121 agcaggaacg ggttggtaca gctctttcgc agagtgggcg tcaccgaact cgaagcccgc
     3181 ggtggaacgc tccccccagc ctcgcagcgt tgggaccgta tcctccaggc atcagggatg
     3241 aaaagggcca aaccgtcccc tacttcagct caaacaccgg atcaggcgtc tttgcatgca
     3301 ttcgccgatt cgctggagcg tgaccttgat gcgcccagcc caatgcacga gggagatcag
     3361 acgcgggcaa gcagccgtaa acggtcccga tcggatcgtg ctgtcaccgg cccctccgca
     3421 cagcaggctg tcgaggtgcg cgttcccgaa cagcgcgatg cgctgcattt gcccctcagc
     3481 tggagggtaa aacgcccgcg taccaggatc tggggcggcc tcccggatcc gggttcagga
     3541 cgggctgacg cattggacga ttttgatctg gatatgctgg gaagtgacgc cctcgatgat
     3601 tttgaccttg acatgcttgg ttcggatgcc cttgatgact ttgacctcga catgctcggc
     3661 agtgacgccc ttgatgattt cgacctggac atgctgatta actctagaag ttccggatct
     3721 ccgaaaaaga aacgcaaagt tggtagccag tacctgcccg acaccgacga ccggcaccgg
     3781 atcgaggaaa agcggaagcg gacctacgag acattcaaga gcatcatgaa gaagtccccc
     3841 ttcagcggcc ccaccgaccc tagacctcca cctagaagaa tcgccgtgcc cagcagatcc
     3901 agcgccagcg tgccaaaacc tgccccccag ccttacccct tcaccagcag cctgagcacc
     3961 atcaactacg acgagttccc taccatggtg ttccccagcg gccagatctc tcaggcctct
     4021 gctctggctc cagcccctcc tcaggtgctg cctcaggctc ctgctcctgc accagctcca
     4081 gccatggtgt ctgcactggc tcaggcacca gcacccgtgc ctgtgctggc tcctggacct
     4141 ccacaggctg tggctccacc agcccctaaa cctacacagg ccggcgaggg cacactgtct
     4201 gaagctctgc tgcagctgca gttcgacgac gaggatctgg gagccctgct gggaaacagc
     4261 accgatcctg ccgtgttcac cgacctggcc agcgtggaca acagcgagtt ccagcagctg
     4321 ctgaaccagg gcatccctgt ggcccctcac accaccgagc ccatgctgat ggaatacccc
     4381 gaggccatca cccggctcgt gacaggcgct cagaggcctc ctgatccagc tcctgcccct
     4441 ctgggagcac caggcctgcc taatggactg ctgtctggcg acgaggactt cagctctatc
     4501 gccgacatgg acttctccgc actgctgggt agcggatcgg gatctcggga ttccagggaa
     4561 gggatgtttt tgccgaagcc tgaggccggc tccgctatta gtgacgtgtt tgagggccgc
     4621 gaggtgtgcc agccaaaacg aatccggcca tttcatcctc caggaagtcc atgggccaac
     4681 cgcccactcc ccgccagcct cgcaccaaca ccaaccggtc cagtacatga gccagtcggg
     4741 tcactgaccc cggcaccagt ccctcagcca ctggatccag cgcccgcagt gactcccgag
     4801 gccagtcacc tgttggagga tcccgatgaa gagactagcc aggctgtcaa agcccttcgg
     4861 gagatggccg atactgtgat tccccagaag gaagaggctg caatctgtgg ccaaatggac
     4921 ctttcccatc cgcccccaag gggccatctg gatgagctga caaccacact tgagtccatg
     4981 accgaggatc tgaacctgga ctcacccctg accccggaat tgaacgagat tctggatacc
     5041 ttcctgaacg acgagtgcct cttgcatgcc atgcatatca gcacaggact gtccatcttc
     5101 gacacatctc tgttctagat gactcgaggt tcgagtatta tggcattggg aaaactgttt
     5161 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     5221 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     5281 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     5341 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     5401 aagttaatat gaggagtaaa acacttgtag ttgtgagctc gcgcgtagtc ctcggtaata
     5461 tcgcagaaca aaagtacctg atatcgagtg tacttcaagt cacaccggcg agtgactagc
     5521 aggtgatgaa aagccttagg gtcaggtggc acttttcggg gaaatgtgcg atgaacccct
     5581 atttgtttat ttttctaaat acattcaaat atgtatccgc taatgagaca ataaccctga
     5641 taaatgcttc aattatattg aaaaaggaag agtatgagta tccaacattt ccgtgtcgcc
     5701 cttattccct tttttgcggc cttctgcctt cctgtttttg ctcacccaga aacgctggtg
     5761 aaagtaaaag atgctgaaga tcagttgggt gcccgagtgg gttacattga actggatctc
     5821 aacagcggca aaatcttaga gagttttcgc cccgaagaac gcttcccaat gatgagcact
     5881 ttcaaagttc tgctatgtgg cgcggtgtta tcccgtattg atgctgggca agagcaactc
     5941 ggtcgccgca tacactattc tcagaatgac ttggttgagt attcaccagt cacagaaaag
     6001 catcttacgg atggcatgac agtaagagaa ttatgtagtg ctgccataac catgagtgat
     6061 aacactgcgg ccaacttact tctgacaacg attggaggac cgaaggagct aaccgctttt
     6121 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6181 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aactctccgc
     6241 aaactattaa ctggcgaact acttactctt gcttcacgcc agcaactcat tgactggatg
     6301 gaggcggaca aagttgcagg accacttctg cgctcggcac ttccggctgg ctggtttatt
     6361 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6421 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6481 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6541 gacgaaggga aataaatagt agcccgcctg atgtgcgggc ttttttttgg tggtggttac
     6601 cagtgtcgct tagctaccgt aggtcctctt aggcggtccg attagcgaca tagtcatcaa
     6661 t
//
