LOCUS       sequence-182066-        2078 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains a fusion of human histone H2B to fluorescent protein mRuby2
            in the pCS2+ expression vector. It has the SP6 RNA polymerase site 
            for in vitro transcription..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2078)
  TITLE     Dr. Philipp Keller Lab
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCS2+ H2B-mRuby2
FEATURES             Location/Qualifiers
     source          1..2078
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..112
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     94..111
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             163..540
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             565..591
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             595..621
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             625..651
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1159..1185
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1192..1218
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1231..1257
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1264..1290
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1474..1500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1504..1530
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1534..1560
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1561..1587
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1591..1617
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1621..1647
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1686..1820
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1710..1729)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1764..1783
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1933..1953)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1933..1951)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1972..1988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1972..1988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1985..2007)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1996..2012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2020..2026,2027..2044,2045..2050))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt ttagggaacc
       61 gccattctgc ctggggacgt cggagcaagc ttgatttagg tgacactata gaatacaagc
      121 tacttgttct ttttgcagga tcccatcgac ggtaccgcca ccatgccaga gccagcgaag
      181 tctgctcccg ccccgaaaaa gggctccaag aaggcggtga ctaaggcgca gaagaaaggc
      241 ggcaagaagc gcaagcgcag ccgcaaggag agctattcca tctatgtgta caaggttctg
      301 aagcaggtcc accctgacac cggcatttcg tccaaggcca tgggcatcat gaattcgttt
      361 gtgaacgaca ttttcgagcg catcgcaggt gaggcttccc gcctggcgca ttacaacaag
      421 cgctcgacca tcacctccag ggagatccag acggccgtgc gcctgctgct gcctggggag
      481 ttggccaagc acgccgtgtc cgagggtact aaggccatca ccaagtacac cagcgctaag
      541 gatccaccgg tcgccaccat gggttatcct tacgatgtcc ctgattacgc tggctatcct
      601 tatgatgtcc cggattatgc tggctatccg tatgatgttc ctgattatgc aggtggaaat
      661 tcgctgatta aggagaatat gagaatgaaa gttgtgctcg aaggctcggt gaatggacat
      721 caattcaaat gcactggaga gggagaaggt aacccgtaca tgggaacaca aactatgcga
      781 attaaggtga ttgagggagg cccacttcct tttgcttttg acatcctcgc aacaagtttt
      841 atgtacggat cgagaacatt tatcaagtat ccaaaaggaa tcccagactt tttcaaacaa
      901 tcattcccgg aaggatttac ttgggaaaga gttacacgat atgaagatgg tggagttatc
      961 accgtgatgc aggatacttc tctcgaagat ggatgtctcg tttaccatgc acaagttaga
     1021 ggagtcaatt ttccatcaaa tggcgctgtt atgcagaaga agacgaaggg atgggaaccg
     1081 aatactgaga tgatgtatcc agctgatgga ggattacgcg gatacacgca catggctttg
     1141 aaagttgatg gtggaggata cccttatgat gtgccagact acgctggagg ttacccttat
     1201 gatgttccag attatgctgg cggaggagga tacccttatg acgttccaga ttatgccgga
     1261 ggttacccgt acgatgtccc ggactacgct ggtggacacc tttcctgctc gttcgtcact
     1321 acatatagat cgaaaaagac cgtgggaaac atcaagatgc caggaatcca tgctgtcgat
     1381 catagactgg aaagacttga agaatccgat aacgagatgt ttgttgttca acgcgagcac
     1441 gcggtcgcta aatttgcagg acttggtggt ggatacccgt atgacgtccc ggattacgct
     1501 ggatacccgt acgatgtccc tgattatgca ggatacccgt atgatgtgcc ggattatgct
     1561 tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga
     1621 tatccatatg acgttccaga ttacgctggc ggatagtcta gaactatagt gagtcgtatt
     1681 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1741 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1801 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     1861 gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc
     1921 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     1981 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2041 aaagtgtaaa gcctggggtg cctaatgagt gagctaac
//
