LOCUS       sequence-182071-        2610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module C, Promoter: none, Gene: BaeI LacZ cassette for GT donor 
            cloning, Terminator: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_C4300
FEATURES             Location/Qualifiers
     source          1..2610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    79..100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(115..120,121..138,139..145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    153..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     158..180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     177..193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     177..193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             189..554
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWNAPCSGASRKNS
                     RATKVPDIECTSSHTGE"
     primer_bind     212..232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        214..232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(279..298)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(280..298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(308..325)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(308..324)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(317..339)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        608..712
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1780..2368
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2269..2288
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2522..2539
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggtaatatc gcagaacaaa agtaccttgg aattttttaa gcttaccggt ggaaagcggg
       61 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      181 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
      241 gctggaggac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattacg
      301 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      361 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      421 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaacgcg ccctgtagcg
      481 gcgcctctag aaaaaattca agagcgacaa aagtacctga tatcgagtgt acttcaagtc
      541 acaccggcga gtgactagca ggtgatgaaa agccttaggg tcaggtggca cttttcgggg
      601 aaatgtgcga tgaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      661 aatgagacaa taaccctgat aaatgcttca attatattga aaaaggaaga gtatgagtat
      721 ccaacatttc cgtgtcgccc ttattccctt ttttgcggcc ttctgccttc ctgtttttgc
      781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cccgagtggg
      841 ttacattgaa ctggatctca acagcggcaa aatcttagag agttttcgcc ccgaagaacg
      901 cttcccaatg atgagcactt tcaaagttct gctatgtggc gcggtgttat cccgtattga
      961 tgctgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     1021 ttcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgtagtgc
     1081 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga ttggaggacc
     1141 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     1201 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     1261 aatggcaaca actctccgca aactattaac tggcgaacta cttactcttg cttcacgcca
     1321 gcaactcatt gactggatgg aggcggacaa agttgcagga ccacttctgc gctcggcact
     1381 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     1441 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1501 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1561 taagcattgg taactgtcag acgaagggaa ataaatagta gcccgcctga tgtgcgggct
     1621 tttttttggt ggtggttacc agtgtcgctt agctaccgta ggtcctctta ggcggtccga
     1681 ttagcgacat agtcatcaat gccttgacgg caaaatccct taacgtgagt tttcgttcca
     1741 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1801 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1861 tcaagagcta ctaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1921 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1981 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2041 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2101 ggggggttcg tgctcacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2161 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2221 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2281 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2341 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2401 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2461 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2521 cagcgagtca gtgagcgagg aagcggaaga gcgcccacct gcaacgccgg cgagctttgg
     2581 gtacgtcacg tggctcgagc gcgtagtcct
//
