LOCUS       sequence-182073-       11605 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For constitutive expression of firefly luciferase from the CAG 
            promoter together with an IRES-linked neo resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11605)
  AUTHORS   Stringer BW, Day BW, D'Souza RCJ, Jamieson PR, Ensbey KS, Bruce ZC, 
            Lim YC, Goasdoue K, Offenhauser C, Akgul S, Allan S, Robertson T, 
            Lucas P, Tollesson G, Campbell S, Winter C, Do H, Dobrovic A, Inglis
            PL, Jeffree RL, Johns TG, Boyd AW
  TITLE     A reference collection of patient-derived cell line and xenograft 
            models of proneural, classical and mesenchymal glioblastoma.
  JOURNAL   Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.1038/s41598-019-41277-z.
  PUBMED    30894629
REFERENCE   2  (bases 1 to 11605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-03-20"; volume: "9(1):4902. doi"; pages: " 
            10.1038/s41598-019-41277-z"
COMMENT     Sequence Label: pF CAG luc IRES neo
FEATURES             Location/Qualifiers
     source          1..11605
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..61
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     123..142
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      173..182
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             179..1831
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(245..263)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_feature    1839..2412
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1862..2414
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2006..2023)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2233..2252
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2477..3271
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2531..2550)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3141..3160
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3328..3916
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3381..3401)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3799..3810)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3919..3935)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3920..3936)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4441..4621
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4647..4664)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4653..4877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4923..5351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5010..5029)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5220..5241
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5360..5380)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5365..5694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5545..5680
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5607..5626
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5742..5789
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5808..6182
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6312..6433
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6349..6368)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6403..6422
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6482..6498)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6482..6498)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6495..6517)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6506..6522
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6530..6536,6537..6554,6555..6560))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6575..6596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6713..6730)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6884..7472)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6964..6983)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7643..8434,8435..8503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8266..8285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8504..8608)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8683..8702)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8874..9253
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9255..9453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9408..9428
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9471..9651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9698..9823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10316..10549
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10734..10778
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11076..11193
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        11301..11604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
       61 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
      121 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaaggatccg ccgccaccat
      181 ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatcctctag aggatggaac
      241 cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc
      301 ttttacagat gcacatatcg aggtgaacat cacgtacgcg gaatacttcg aaatgtccgt
      361 tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg
      421 cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc
      481 agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta tgaacatttc
      541 gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa
      601 aaaattacca ataatccaga aaattattat catggattct aaaacggatt accagggatt
      661 tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt
      721 tgtaccagag tcctttgatc gtgacaaaac aattgcactg ataatgaatt cctctggatc
      781 tactgggtta cctaagggtg tggcccttcc gcatagaact gcctgcgtca gattctcgca
      841 tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt
      901 tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg
      961 agtcgtctta atgtatagat ttgaagaaga gctgttttta cgatcccttc aggattacaa
     1021 aattcaaagt gcgttgctag taccaaccct attttcattc ttcgccaaaa gcactctgat
     1081 tgacaaatac gatttatcta atttacacga aattgcttct gggggcgcac ctctttcgaa
     1141 agaagtcggg gaagcggttg caaaacgctt ccatcttcca gggatacgac aaggatatgg
     1201 gctcactgag actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc
     1261 ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac
     1321 gctgggcgtt aatcagagag gcgaattatg tgtcagagga cctatgatta tgtccggtta
     1381 tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg
     1441 agacatagct tactgggacg aagacgaaca cttcttcata gttgaccgct tgaagtcttt
     1501 aattaaatac aaaggatatc aggtggcccc cgctgaattg gaatcgatat tgttacaaca
     1561 ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac gatgacgccg gtgaacttcc
     1621 cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta
     1681 cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga
     1741 agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa
     1801 ggccaagaag ggcggaaagt ccaaattgta agctagcgcc cctctccctc ccccccccct
     1861 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     1921 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     1981 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     2041 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     2101 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     2161 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     2221 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     2281 gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac atgtgtttag
     2341 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     2401 acacgatgat aatatggcca caaaccggtc ttgattcttc tgacacaaca gtctcgaact
     2461 taaggctaga gccaccatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2521 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2581 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2641 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2701 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2761 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2821 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2881 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2941 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3001 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3061 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3121 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3181 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3241 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaggcgc
     3301 gccgaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
     3361 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3421 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3481 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3541 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3601 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3661 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3721 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3781 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3841 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3901 tgggccgcct ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc
     3961 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag
     4021 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
     4081 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4141 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
     4201 tggcagaact acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac
     4261 aagctagtac cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc
     4321 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg
     4381 aggtttgaca gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact
     4441 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4501 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4561 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4621 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4681 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4741 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4801 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4861 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4921 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4981 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     5041 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     5101 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     5161 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5221 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     5281 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     5341 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5401 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5521 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5581 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     5641 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     5701 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     5761 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     5821 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     5881 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     5941 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     6001 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     6061 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     6121 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     6181 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6241 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6301 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6361 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6421 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     6481 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6541 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6601 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6661 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     8641 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     8701 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8761 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8821 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     8881 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     8941 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     9001 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     9061 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     9121 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     9181 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     9241 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     9301 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     9361 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     9421 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     9481 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9541 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9601 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9661 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     9721 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     9781 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     9841 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     9901 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     9961 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10021 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10081 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10141 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
    10201 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
    10261 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
    10321 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
    10381 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    10441 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    10501 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    10561 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    10621 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    10681 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    10741 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    10801 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    10861 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    10921 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    10981 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
    11041 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
    11101 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
    11161 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
    11221 ccagtttggt taattaagac tagttattaa tagtaatcaa ttacggggtc attagttcat
    11281 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
    11341 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
    11401 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
    11461 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
    11521 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
    11581 gtattagtca tcgctattac catgg
//
