LOCUS       sequence-182076-       10147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: none, Viral 
            Replication: BeYDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_201
FEATURES             Location/Qualifiers
     source          1..10147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    1991..2115
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2140..2145,2146..2163,2164..2170)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2224..2904
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             3224..3529
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3408..3427
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3570..3694)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5377..5401
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6702..7331
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7760..8833
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8899..9093
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9329..9351)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9437..9577
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9492..9511
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9592..9609)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9763..10147,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9843..9862)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtagagctc aaagtttaac gcgttagcag aaggcatgtt
     1621 gttgtgactc cgaggggttg cctcaaactc tatcttataa ccggcgtgga ggcatggagg
     1681 caggggtatt ttggtcattt taatagatag tggaaaatga cgtggaattt acttaaagac
     1741 gaagtctttg cgacaagggg gggcccacgc cgaatttaat attaccggcg tggccccccc
     1801 ttatcgcgag tgctttagca cgagcggtcc agatttaaag tagaaaattt cccgcccact
     1861 agggttaaag gtgttcacac tataaaagca tatacgatgt gatggtattt gatggagcgt
     1921 atattgtatc aggtatttcc gttggatacg aattattcgt acgaccctcg gtaccgatca
     1981 aaagcaggtg acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     2041 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     2101 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     2161 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     2221 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     2281 gaacattttg aggcatttca gtcagtcgct caatgtacct ataaccagac cgttcagctg
     2341 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     2401 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     2461 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     2521 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     2581 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     2641 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     2701 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     2761 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     2821 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca ggcggggcgt
     2881 aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     2941 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     3001 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     3061 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     3121 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     3181 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     3241 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     3301 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     3361 cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     3421 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     3481 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     3541 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     3601 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     3661 acgtttctcg ttcagctttc ttgtacaaag tggtcacctg caaaaagtgt ttgatcgccg
     3721 gcggtaccga gtgtacttca agtcagtggg aaatcaataa aatgattatt ttatgaatat
     3781 atttcattgt gcaagtagat agaaattaca tatgttacat aacacacgaa ataaacaaaa
     3841 aaagacaatc caaaaacaaa caccccaaaa aaaataatca ctttagataa actcgtatga
     3901 ggagaggcac gttcagtgac tcgacgattc ccgagcaaaa aaagtctccc cgtcacacat
     3961 gtagtgggtg acgcaattat ctttaaagta atccttctgt tgacttgtca ttgataacat
     4021 ccagtcttcg tcaggattgc aaagaattat agaagggatc ccacctttta ttttcttctt
     4081 ttttccatat ttagggttga cagtgaaatc agactggcaa cctattaatt gcttccacaa
     4141 tgggacgaac ttgaagggga tgtcgtcgat gatattatag gtggcgtgtt catcgtagtt
     4201 ggtgaaatcg atggtaccgt tccaatagtt gtgtcgtccg agacttctag cccaggtggt
     4261 ctttccggta cgagttggtc cgcagatgta gaggctgggg tgtcggattc cattccttcc
     4321 attgtccttg ttaaatcggc catccattca aggtcagatt gagcttgttg gtatgagaca
     4381 ggatgtatgt aagtataagc gtctatgctt acatggtata gatgggtttc cctccaggag
     4441 tgtagatctt cgtggcagcg aagatctgat tctgtgaagg gcgacacata cggttcaggt
     4501 tgtggaggga ataatttgtt ggctgaatat tccagccatt gaagctttgt tgcccattca
     4561 tgagggaatt cttccttgat catgtcaaga tattcctcct tagacgttgc agtctggata
     4621 atagttctcc atcgtgcgtc agatttgcga ggagaaacct tatgatctcg gaaatctcct
     4681 ctggttttaa tatctccgtc ctttgatatg taatcaagga cttgtttaga gtttctagct
     4741 ggctggatat tagggtgatt tccttcaaaa tcgaaaaaag aaggatccct aatacaaggt
     4801 tttttatcaa gctggagaag agcatgatag tgggtagtgc catcttgatg aagctcagaa
     4861 gcaacaccaa ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa ctggaataaa
     4921 tcatctcttt gagatgagca cttgggatag gtaaggaaaa catatttaga ttggagtctg
     4981 aagttcttac tagcagaagg catgttgttg tgactccgag gggttgcctc aaactctatc
     5041 ttataaccgg cgtggaggca tggaggcagg ggtattttgg tcattttaat agatagtgga
     5101 aaatgacgtg gaatttactt aaagacgaag tctttgcgac aagggggggc ccacgccgaa
     5161 tttaatatta ccggcgtggc ccccccttat cgcgagtgct ttagcacgag cggtccagat
     5221 ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt tcacactata aaagcatata
     5281 cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt atttccgttg gatacgaatt
     5341 attcgtacga ccctcatagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     5401 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     5461 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac
     5521 tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
     5581 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
     5641 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
     5701 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
     5761 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
     5821 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
     5881 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
     5941 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
     6001 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
     6061 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
     6121 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
     6181 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
     6241 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
     6301 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
     6361 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
     6421 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
     6481 gtgttcgagc cgcccgcgca cggctcaacc gtgcggctgc atgaaatcct ggccggtttg
     6541 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
     6601 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     6661 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     6721 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     6781 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     6841 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     6901 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     6961 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     7021 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     7081 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     7141 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     7201 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     7261 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     7321 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     7381 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     7441 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     7501 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     7561 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     7621 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     7681 gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc actggaaccc
     7741 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     7801 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     7861 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     7921 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     7981 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     8041 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     8101 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     8161 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     8221 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     8281 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     8341 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     8401 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     8461 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     8521 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     8581 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     8641 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     8701 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     8761 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     8821 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     8881 tcgaaaaggc ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     8941 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     9001 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     9061 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     9121 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     9181 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     9241 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     9301 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gaaacggtca
     9361 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9421 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     9481 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9541 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     9601 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9661 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9721 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9781 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9841 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9901 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9961 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10021 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10081 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10141 gaggtat
//
