LOCUS       sequence-182077-       10565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: none, Viral 
            Replication: ToLCV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_202
FEATURES             Location/Qualifiers
     source          1..10565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    1932..2056
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2081..2086,2087..2104,2105..2111)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2165..2845
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             3165..3470
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3349..3368
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3511..3635)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(3672..3924)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5795..5819
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7120..7749
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8178..9251
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9317..9511
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(9747..9769)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9855..9995
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9910..9929
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10010..10027)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10181..10565,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10261..10280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtagagctc aaagtttaac gcgtcgatta agttgacttg
     1621 gtcaatcggt gtctctcaac tctctctatg tatcggtgta ttggagtcct atatatatgg
     1681 agactctaat ggcataaatg taaatatgga actttaaatc aaaaccctta cgctccaaaa
     1741 agcggccatc cgtataatat taccggatgg ccgcgatttt ttttttggac cccacaacgc
     1801 aataactgac aaagacatgc ggaccaatca cctgacgcgc tcaaagctta attgttttgt
     1861 gttttctatt taaacttggg caccaagtat tgtagttcgt actacaagtt tgtaccgatc
     1921 aaaagcaggt gacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     1981 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     2041 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     2101 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     2161 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     2221 agaacatttt gaggcatttc agtcagtcgc tcaatgtacc tataaccaga ccgttcagct
     2281 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     2341 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     2401 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     2461 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     2521 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     2581 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     2641 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2701 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     2761 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg
     2821 taatctagag gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     2881 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     2941 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     3001 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     3061 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     3121 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     3181 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     3241 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     3301 tcccccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     3361 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     3421 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     3481 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     3541 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     3601 tacgtttctc gttcagcttt cttgtacaaa gtggtcacct gcaaaaagtg tttgatcgcc
     3661 ggcggtacaa agtggtagta acatagatga caccgcgcgc gataatttat cctagtttgc
     3721 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     3781 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     3841 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     3901 ttattgccaa atgtttgaac gatcggggaa attcctaata aatattaaat tttattatat
     3961 gcgaactgtc tacaaatgtt gtttgcgtca atgcatccca tagtacataa ttcactgctc
     4021 taattacatt attcaaacta attacaccca aattattaag aaatttcaac acttgtgttt
     4081 taaataccct taagaaacga ccagtcggag gctgtgaggt catccagatt cggtaggtta
     4141 ggaaacattt gtgtatcccc aacactttcc tcaggttgtg attgaactgt atccggacgg
     4201 tgatgatgtc ttcgttcatc acgaagggcc ggttttggtg ctctgttatc ttgaaatata
     4261 ggggattttg aatctcccag ataaacacgc cattctctgc ttgagctgca gtgatgagtt
     4321 cccctgtgcg tgaatccatg gtcgtggcag gttattgcta tgaagtaaga acagccacac
     4381 ggtagatcaa ctcgtcgacg tctggtcccc ttcttggcta gcctgtgctg tactttgatt
     4441 ggtacctgag tagagtgggc cttcgagggt gacgaaggtc gcatttttta aagcccagtt
     4501 ttttagtgca gaattctttt cctcatccaa gtattcttta tagctggagt tgggtcctgg
     4561 attgcagagg aaggtagtgg gaattccgcc tttaatttga actggcttcc cgtactttgt
     4621 atttgattgc cagtcccttt gggcccccat gaattcttta aagtgcttta ggtagtgggg
     4681 gtctacgtca tcaatgacgt tgtaccaggc atcattactg taaacctttg ggctaaggtc
     4741 tagatgacca cacaaataat tatgtggtcc caatgatctg gcccacatag tcttccccgt
     4801 cctactgtca ccctcaatta ctatacttat gggtctcaga ggccgcgcag cgggactgca
     4861 gacgttctcg gcagcccact cttcaagttc ttctggaact tggtcgaaag aagaagataa
     4921 aaaaggtgaa acataaacat ccattggagg tgtaaaaatc ctatctaaat tagaatttaa
     4981 attgtgaaat tgtaaaacaa aatctttggg agctttctcc ctcaatatat taagggcctg
     5041 agctttggac cctgaattga ttgcctcggc atatgcgtcg ttggcagatt ggcaacctcc
     5101 tctagctgat cgtccatcga cttggaaaac tccatgatca atgacgtctc cgtctttttc
     5161 catgtaggat ttgacatcgc ttgagctctt agctccctga atgttcggat ggaaatgtgc
     5221 tgacctggtt ggggatgtga ggtcgaagaa tctattgttt ttgcactgga actttccttc
     5281 gaactggatg agcacatgca agtgaggagt cccatcttcg tgaagctctc tgcaaattct
     5341 aataaatttt ttgaaagtgg gtgtttggag atttaataat ttgggaaagt gctcttcttt
     5401 agttagagag cacttgggat aagtgagaaa ataatttttg gcatttattt taaagcgatt
     5461 gggggctgcc atgttgactt ggtcaatcgg tgtctctcaa ctctctctat gtatcggtgt
     5521 attggagtcc tatatatatg gagactctaa tggcataaat gtaaatatgg aactttaaat
     5581 caaaaccctt acgctccaaa aagcggccat ccgtataata ttaccggatg gccgcgattt
     5641 tttttttgga ccccacaacg caataactga caaagacatg cggaccaatc acctgacgcg
     5701 ctcaaagctt aattgttttg tgttttctat ttaaacttgg gcaccaagta ttgtagttcg
     5761 tacttggggc cctcgagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     5821 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     5881 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     5941 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     6001 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     6061 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     6121 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     6181 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     6241 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     6301 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     6361 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     6421 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     6481 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     6541 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     6601 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     6661 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     6721 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     6781 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     6841 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     6901 gttcgagccg cccgcgcacg gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     6961 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     7021 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     7081 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     7141 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     7201 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     7261 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     7321 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     7381 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     7441 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     7501 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     7561 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     7621 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     7681 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     7741 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     7801 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     7861 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     7921 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     7981 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     8041 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     8101 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
     8161 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
     8221 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
     8281 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
     8341 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
     8401 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
     8461 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
     8521 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
     8581 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
     8641 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
     8701 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
     8761 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
     8821 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
     8881 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
     8941 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
     9001 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
     9061 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
     9121 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
     9181 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
     9241 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
     9301 gaaaaggcct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
     9361 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
     9421 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
     9481 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
     9541 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
     9601 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
     9661 gcggacaagc cgcgccgtcg ccactcgaca gccggcgccc acatcaaggc accctgcctc
     9721 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga aacggtcaca
     9781 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     9841 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     9901 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     9961 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    10021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    10081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    10141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    10201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    10261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    10321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    10381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    10441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    10501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    10561 ggtat
//
