LOCUS       sequence-182086-       12477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral 
            Replication: BeYDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12477)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_211
FEATURES             Location/Qualifiers
     source          1..12477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(2919..3595)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2962..2982)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3786..3807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3822..3827,3828..3845,3846..3852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3860..3876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3865..3887
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3884..3900
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3884..3900
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4321..4445
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4470..4475,4476..4493,4494..4500)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4554..5234
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5554..5859
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5738..5757
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5900..6024)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7707..7731
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9032..9661
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10090..11163
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11229..11423
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11659..11681)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11767..11907
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11822..11841
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11922..11939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12093..12477,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12173..12192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     2581 atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag
     2881 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     2941 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     3001 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     3061 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     3121 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     3181 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     3241 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     3301 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg
     3361 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     3421 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     3481 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     3541 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     3601 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     3661 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
     3721 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3781 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     3841 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     3901 atgattacga attcgagctc aaagtttaac gcgttagcag aaggcatgtt gttgtgactc
     3961 cgaggggttg cctcaaactc tatcttataa ccggcgtgga ggcatggagg caggggtatt
     4021 ttggtcattt taatagatag tggaaaatga cgtggaattt acttaaagac gaagtctttg
     4081 cgacaagggg gggcccacgc cgaatttaat attaccggcg tggccccccc ttatcgcgag
     4141 tgctttagca cgagcggtcc agatttaaag tagaaaattt cccgcccact agggttaaag
     4201 gtgttcacac tataaaagca tatacgatgt gatggtattt gatggagcgt atattgtatc
     4261 aggtatttcc gttggatacg aattattcgt acgaccctcg gtaccgatca aaagcaggtg
     4321 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
     4381 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
     4441 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
     4501 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
     4561 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     4621 aggcatttca gtcagtcgct caatgtacct ataaccagac cgttcagctg gatattacgg
     4681 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     4741 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     4801 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     4861 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     4921 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     4981 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     5041 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     5101 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
     5161 gaatgcttaa tgaattacaa cagtactgcg atgagtggca ggcggggcgt aatctagagg
     5221 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
     5281 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag
     5341 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
     5401 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
     5461 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
     5521 gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa
     5581 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
     5641 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt cccccgtgaa
     5701 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
     5761 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
     5821 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
     5881 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
     5941 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     6001 ttcagctttc ttgtacaaag tggtcacctg caaaaagtgt ttgatcgccg gcggtaccga
     6061 gtgtacttca agtcagtggg aaatcaataa aatgattatt ttatgaatat atttcattgt
     6121 gcaagtagat agaaattaca tatgttacat aacacacgaa ataaacaaaa aaagacaatc
     6181 caaaaacaaa caccccaaaa aaaataatca ctttagataa actcgtatga ggagaggcac
     6241 gttcagtgac tcgacgattc ccgagcaaaa aaagtctccc cgtcacacat gtagtgggtg
     6301 acgcaattat ctttaaagta atccttctgt tgacttgtca ttgataacat ccagtcttcg
     6361 tcaggattgc aaagaattat agaagggatc ccacctttta ttttcttctt ttttccatat
     6421 ttagggttga cagtgaaatc agactggcaa cctattaatt gcttccacaa tgggacgaac
     6481 ttgaagggga tgtcgtcgat gatattatag gtggcgtgtt catcgtagtt ggtgaaatcg
     6541 atggtaccgt tccaatagtt gtgtcgtccg agacttctag cccaggtggt ctttccggta
     6601 cgagttggtc cgcagatgta gaggctgggg tgtcggattc cattccttcc attgtccttg
     6661 ttaaatcggc catccattca aggtcagatt gagcttgttg gtatgagaca ggatgtatgt
     6721 aagtataagc gtctatgctt acatggtata gatgggtttc cctccaggag tgtagatctt
     6781 cgtggcagcg aagatctgat tctgtgaagg gcgacacata cggttcaggt tgtggaggga
     6841 ataatttgtt ggctgaatat tccagccatt gaagctttgt tgcccattca tgagggaatt
     6901 cttccttgat catgtcaaga tattcctcct tagacgttgc agtctggata atagttctcc
     6961 atcgtgcgtc agatttgcga ggagaaacct tatgatctcg gaaatctcct ctggttttaa
     7021 tatctccgtc ctttgatatg taatcaagga cttgtttaga gtttctagct ggctggatat
     7081 tagggtgatt tccttcaaaa tcgaaaaaag aaggatccct aatacaaggt tttttatcaa
     7141 gctggagaag agcatgatag tgggtagtgc catcttgatg aagctcagaa gcaacaccaa
     7201 ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa ctggaataaa tcatctcttt
     7261 gagatgagca cttgggatag gtaaggaaaa catatttaga ttggagtctg aagttcttac
     7321 tagcagaagg catgttgttg tgactccgag gggttgcctc aaactctatc ttataaccgg
     7381 cgtggaggca tggaggcagg ggtattttgg tcattttaat agatagtgga aaatgacgtg
     7441 gaatttactt aaagacgaag tctttgcgac aagggggggc ccacgccgaa tttaatatta
     7501 ccggcgtggc ccccccttat cgcgagtgct ttagcacgag cggtccagat ttaaagtaga
     7561 aaatttcccg cccactaggg ttaaaggtgt tcacactata aaagcatata cgatgtgatg
     7621 gtatttgatg gagcgtatat tgtatcaggt atttccgttg gatacgaatt attcgtacga
     7681 ccctcatagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa
     7741 aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt
     7801 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
     7861 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
     7921 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
     7981 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
     8041 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
     8101 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     8161 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     8221 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     8281 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     8341 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     8401 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     8461 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     8521 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     8581 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     8641 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     8701 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     8761 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     8821 cgcccgcgca cggctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     8881 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     8941 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     9001 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     9061 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     9121 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     9181 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     9241 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     9301 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     9361 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     9421 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     9481 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     9541 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     9601 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     9661 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     9721 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     9781 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     9841 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     9901 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     9961 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
    10021 aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc actggaaccc ccaagcccga
    10081 ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt
    10141 gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg catcgaggca
    10201 gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg
    10261 caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga cgagcaacca
    10321 gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag catcatggac
    10381 gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag
    10441 cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag tgtgtgggat
    10501 tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa
    10561 gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc
    10621 tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg cattcggtta
    10681 aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg cctggtgacg
    10741 gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga aaccgggcgg
    10801 ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac agaaggcaag
    10861 aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg catcggccgt
    10921 tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg gttgttcaag
    10981 acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt caccgtgcgc
    11041 aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc ggggcaggct
    11101 ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc
    11161 taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc
    11221 ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg gaaccggaac
    11281 ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta agtgactgat
    11341 ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat taaaactctt
    11401 aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagcccaaga gctgcaaaaa
    11461 gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg
    11521 gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg gcgcggacaa
    11581 gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt
    11641 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gaaacggtca cagcttgtct
    11701 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    11761 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
    11821 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
    11881 tgcgtaagga gaaaataccg catcaggccc tcttccgctt cctcgctcac tgactcgctg
    11941 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12001 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12061 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12121 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12181 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12241 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12301 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12361 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12421 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtat
//
