LOCUS       sequence-182088-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CRISPR base editor in protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, Qiu JL, Wang D, Gao C
  TITLE     Precise base editing in rice, wheat and maize with a Cas9-cytidine 
            deaminase fusion.
  JOURNAL   Nat Biotechnol. 2017 May;35(5):438-440. doi: 10.1038/nbt.3811. Epub 
            2017 Feb 27.
  PUBMED    28244994
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3811"; 
            journalName: "Nat Biotechnol"; date: "2017-05"; volume: "35"; issue:
            "5"; pages: "438-440"
COMMENT     Sequence Label: pnCas9-PBE
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2034..2054
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2055..2738
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGG
                     RHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLS
                     RYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNE
                     AHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHIL
                     WATGLK"
     CDS             join(2790..2813,2814..2816,2817..6890)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6891..6938
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6960..7208
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7221..7241
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7315..7491
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8045..8061)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8045..8061)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8058..8080)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8069..8085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8093..8099,8100..8117,8118..8123))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8138..8159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8276..8293)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8447..9035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8527..8546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9206..9997,9998..10066))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9829..9848
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10067..10171)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccaagctaa ttcgagctcg gtacctgacc cggtcgtgcc cctctctaga gataatgagc
       61 attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact tgtttgaagt
      121 gcagtttatc tatctttata catatattta aactttactc tacgaataat ataatctata
      181 gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag acatggtcta
      241 aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta gtgtgcatgt
      301 gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat tttattagta
      361 catccattta gggtttaggg ttaatggttt ttatagacta atttttttag tacatctatt
      421 ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt tttttattta
      481 ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa atacccttta
      541 agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg ccagcctgtt
      601 aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc gcgtcgggcc
      661 aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc gatcgagagt
      721 tccgctccac cgttggactt gctccgctgt cggcatccag aaattgcgtg gcggagcggc
      781 agacgtgagc cggcacggca ggcggcctcc tcctcctctc acggcaccgg cagctacggg
      841 ggattccttt cccaccgctc cttcgctttc ccttcctcgc ccgccgtaat aaatagacac
      901 cccctccaca ccctctttcc ccaacctcgt gttgttcgga gcgcacacac acacaaccag
      961 atctccccca aatccacccg tcggcacctc cgcttcaagg tacgccgctc gtcctccccc
     1021 cccccccctc tctaccttct ctagatcggc gttccggtcc atggttaggg cccggtagtt
     1081 ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt
     1141 cgtacacgga tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct
     1201 ctttggggaa tcctgggatg gctctagccg ttccgcagac gggatcgatt tcatgatttt
     1261 ttttgtttcg ttgcataggg tttggtttgc ccttttcctt tatttcaata tatgccgtgc
     1321 acttgtttgt cgggtcatct tttcatgctt ttttttgtct tggttgtgat gatgtggtct
     1381 ggttgggcgg tcgttctaga tcggagtaga attaattctg tttcaaacta cctggtggat
     1441 ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga attgaagatg
     1501 atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt actgatgcat
     1561 atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg gcggtcgttc
     1621 attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt attaattttg
     1681 gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga tggaaatatc
     1741 gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca tgatggcata
     1801 tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata aacaagtatg
     1861 ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca gctatatgtg
     1921 gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt cttttgtcga
     1981 tgctcaccct gttgtttggt gttacttctg caaagcttgt cgacggatcc atgccaaaga
     2041 agaagaggaa ggtttcatcg gagaccggcc ctgttgctgt tgaccccacc ctgcggcgga
     2101 gaatcgagcc acacgagttc gaggtgttct tcgacccaag ggagctccgc aaggagacgt
     2161 gcctcctgta cgagatcaac tggggcggca ggcactccat ctggaggcac accagccaaa
     2221 acaccaacaa gcacgtggag gtcaacttca tcgagaagtt caccaccgag aggtacttct
     2281 gcccaaacac ccgctgctcc atcacctggt tcctgtcctg gagcccatgc ggcgagtgct
     2341 ccagggccat caccgagttc ctcagccgct acccacacgt caccctgttc atctacatcg
     2401 ccaggctcta ccaccacgcc gacccaagga acaggcaggg cctccgcgac ctgatctcca
     2461 gcggcgtgac catccaaatc atgaccgagc aggagtccgg ctactgctgg aggaacttcg
     2521 tcaactactc cccaagcaac gaggcccact ggccaaggta cccacacctc tgggtgcgcc
     2581 tctacgtgct cgagctgtac tgcatcatcc tcggcctgcc accatgcctc aacatcctga
     2641 ggcgcaagca accacagctg accttcttca ccatcgccct ccaaagctgc cactaccaga
     2701 ggctcccacc acacatcctg tgggctaccg gcctcaagtc cggcagcgag acgccaggca
     2761 cctccgagag cgctacgcct gaacttaagg acaagaagta ctcgatcggc ctcgccatcg
     2821 ggacgaactc agttggctgg gccgtgatca ccgacgagta caaggtgccc tctaagaagt
     2881 tcaaggtcct ggggaacacc gaccgccatt ccatcaagaa gaacctcatc ggcgctctcc
     2941 tgttcgacag cggggagacc gctgaggcta cgaggctcaa gagaaccgct aggcgccggt
     3001 acacgagaag gaagaacagg atctgctacc tccaagagat tttctccaac gagatggcca
     3061 aggttgacga ttcattcttc caccgcctgg aggagtcttt cctcgtggag gaggataaga
     3121 agcacgagcg gcatcccatc ttcggcaaca tcgtggacga ggttgcctac cacgagaagt
     3181 accctacgat ctaccatctg cggaagaagc tcgtggactc caccgataag gcggacctca
     3241 gactgatcta cctcgctctg gcccacatga tcaagttccg cggccatttc ctgatcgagg
     3301 gggatctcaa cccagacaac agcgatgttg acaagctgtt catccaactc gtgcagacct
     3361 acaaccaact cttcgaggag aacccgatca acgcctctgg cgtggacgcg aaggctatcc
     3421 tgtccgcgag gctctcgaag tccaggaggc tggagaacct gatcgctcag ctcccaggcg
     3481 agaagaagaa cggcctgttc gggaacctca tcgctctcag cctggggctc accccgaact
     3541 tcaagtcgaa cttcgatctc gctgaggacg ccaagctgca actctccaag gacacctacg
     3601 acgatgacct cgataacctc ctggcccaga tcggcgatca atacgcggac ctgttcctcg
     3661 ctgccaagaa cctgtcggac gccatcctcc tgtcagatat cctccgcgtg aacaccgaga
     3721 tcacgaaggc tccactctct gcctccatga tcaagcgcta cgacgagcac catcaggatc
     3781 tgaccctcct gaaggcgctg gtccgccaac agctcccgga gaagtacaag gagattttct
     3841 tcgatcagtc gaagaacggc tacgctgggt acatcgacgg cggggcctca caagaggagt
     3901 tctacaagtt catcaagcca atcctggaga agatggacgg cacggaggag ctcctggtga
     3961 agctcaacag ggaggacctc ctgcggaagc agagaacctt cgataacggc agcatccccc
     4021 accaaatcca tctcggggag ctgcacgcca tcctgagaag gcaagaggac ttctaccctt
     4081 tcctcaagga taaccgggag aagatcgaga agatcctgac cttcagaatc ccatactacg
     4141 tcggccctct cgcgcggggg aactcaagat tcgcttggat gacccgcaag tctgaggaga
     4201 ccatcacgcc gtggaacttc gaggaggtgg tggacaaggg cgctagcgct cagtcgttca
     4261 tcgagaggat gaccaacttc gacaagaacc tgcccaacga gaaggtgctc cctaagcact
     4321 cgctcctgta cgagtacttc accgtctaca acgagctcac gaaggtgaag tacgtcaccg
     4381 agggcatgcg caagccagcg ttcctgtccg gggagcagaa gaaggctatc gtggacctcc
     4441 tgttcaagac caaccggaag gtcacggtta agcaactcaa ggaggactac ttcaagaaga
     4501 tcgagtgctt cgattcggtc gagatcagcg gcgttgagga ccgcttcaac gccagcctcg
     4561 ggacctacca cgatctcctg aagatcatca aggataagga cttcctggac aacgaggaga
     4621 acgaggatat cctggaggac atcgtgctga ccctcacgct gttcgaggac agggagatga
     4681 tcgaggagcg cctgaagacg tacgcccatc tcttcgatga caaggtcatg aagcaactca
     4741 agcgccggag atacaccggc tgggggaggc tgtcccgcaa gctcatcaac ggcatccggg
     4801 acaagcagtc cgggaagacc atcctcgact tcctcaagag cgatggcttc gccaacagga
     4861 acttcatgca actgatccac gatgacagcc tcaccttcaa ggaggatatc caaaaggctc
     4921 aagtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcccccg
     4981 cgatcaagaa gggcatcctc cagaccgtga aggttgtgga cgagctcgtg aaggtcatgg
     5041 gccggcacaa gcctgagaac atcgtcatcg agatggccag agagaaccaa accacgcaga
     5101 aggggcaaaa gaactctagg gagcgcatga agcgcatcga ggagggcatc aaggagctgg
     5161 ggtcccaaat cctcaaggag cacccagtgg agaacaccca actgcagaac gagaagctct
     5221 acctgtacta cctccagaac ggcagggata tgtacgtgga ccaagagctg gatatcaacc
     5281 gcctcagcga ttacgacgtc gatcatatcg ttccccagtc tttcctgaag gatgactcca
     5341 tcgacaacaa ggtcctcacc aggtcggaca agaaccgcgg caagtcagat aacgttccat
     5401 ctgaggaggt cgttaagaag atgaagaact actggaggca gctcctgaac gccaagctga
     5461 tcacgcaaag gaagttcgac aacctcacca aggctgagag aggcgggctc tcagagctgg
     5521 acaaggccgg cttcatcaag cggcagctgg tcgagaccag acaaatcacg aagcacgttg
     5581 cgcaaatcct cgactctcgg atgaacacga agtacgatga gaacgacaag ctgatcaggg
     5641 aggttaaggt gatcaccctg aagtctaagc tcgtctccga cttcaggaag gatttccagt
     5701 tctacaaggt tcgcgagatc aacaactacc accatgccca tgacgcttac ctcaacgctg
     5761 tggtcggcac cgctctgatc aagaagtacc caaagctgga gtccgagttc gtgtacgggg
     5821 actacaaggt ttacgatgtg cgcaagatga tcgccaagtc ggagcaagag atcggcaagg
     5881 ctaccgccaa gtacttcttc tactcaaaca tcatgaactt cttcaagacc gagatcacgc
     5941 tggccaacgg cgagatccgg aagagaccgc tcatcgagac caacggcgag acgggggaga
     6001 tcgtgtggga caagggcagg gatttcgcga ccgtccgcaa ggttctctcc atgccccagg
     6061 tgaacatcgt caagaagacc gaggtccaaa cgggcgggtt ctcaaaggag tctatcctgc
     6121 ctaagcggaa cagcgacaag ctcatcgcca gaaagaagga ctgggaccca aagaagtacg
     6181 gcgggttcga cagccctacc gtggcctact cggtcctggt tgtggcgaag gttgagaagg
     6241 gcaagtccaa gaagctcaag agcgtgaagg agctcctggg gatcaccatc atggagaggt
     6301 ccagcttcga gaagaaccca atcgacttcc tggaggccaa gggctacaag gaggtgaaga
     6361 aggacctgat catcaagctc ccgaagtact ctctcttcga gctggagaac ggcaggaaga
     6421 gaatgctggc ttccgctggc gagctccaga aggggaacga gctcgcgctg ccaagcaagt
     6481 acgtgaactt cctctacctg gcttcccact acgagaagct caagggcagc ccggaggaca
     6541 acgagcaaaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcatcgagc
     6601 aaatctccga gttcagcaag cgcgtgatcc tcgccgacgc gaacctggat aaggtcctct
     6661 ccgcctacaa caagcaccgg gacaagccca tcagagagca agcggagaac atcatccatc
     6721 tcttcaccct gacgaacctc ggcgctcctg ctgctttcaa gtacttcgac accacgatcg
     6781 atcggaagag atacacctcc acgaaggagg tcctggacgc gaccctcatc caccagtcga
     6841 tcaccggcct gtacgagacg aggatcgacc tctcacaact cggcggggat aagagacccg
     6901 cagcaaccaa gaaggcaggg caagcaaaga agaagaagac gcgtgactcc ggcggcagca
     6961 ccaacctgtc cgacatcatc gagaaggaga cgggcaagca actcgtgatc caggagagca
     7021 tcctcatgct gccagaggag gtggaggagg tcatcggcaa caagccagag tccgacatcc
     7081 tggtgcacac cgcctacgac gagtccaccg acgagaacgt catgctcctg accagcgacg
     7141 ccccagagta caagccatgg gccctcgtca tccaggacag caacggggag aacaagatca
     7201 agatgctgtc gggggggagc ccaaagaaga agcggaaggt gtagtgtaca agtaacccgg
     7261 gaattcggta cgctgaaatc accagtctct ctctacaaat ctatctctct ctattttctc
     7321 cataaataat gtgtgagtag tttcccgata agggaaatta gggttcttat agggtttcgc
     7381 tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat
     7441 caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat ctcctaaagt
     7501 ccctatagat ctttgtcgtg aatataaacc agacacgaga cgactaaacc tggagcccag
     7561 acgccgttcg aagctagaag taccgcttag gcaggaggcc gttagggaaa agatgctaag
     7621 gcagggttgg ttacgttgac tcccccgtag gtttggttta aatatgatga agtggacgga
     7681 aggaaggagg aagacaagga aggataaggt tgcaggccct gtgcaaggta agaagatgga
     7741 aatttgatag aggtacgcta ctatacttat actatacgct aagggaatgc ttgtatttat
     7801 accctatacc ccctaataac cccttatcaa tttaagaaat aatccgcata agcccccgct
     7861 taaaaattgg tatcagagcc atgaataggt ctatgaccaa aactcaagag gataaaacct
     7921 caccaaaata cgaaagagtt cttaactcta aagataaaag atctttcaag atcaaaacta
     7981 gttccctcac accggtgacg gggatcgcat gcgatatctc gagatctagc ttggcgtaat
     8041 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     8101 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8161 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8221 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8281 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8341 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8401 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8461 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8521 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8581 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8641 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8701 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8761 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8821 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8881 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8941 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9001 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9061 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9121 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9181 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9241 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9301 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9361 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9421 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9481 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9541 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9601 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9661 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9721 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9781 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9841 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9901 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9961 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10021 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10081 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10141 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcca
    10201 gt
//
