LOCUS       sequence-182091-       10442 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral 
            Replication: none.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_220d
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        72..748
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     685..705
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             811..1608
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(868..887)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1478..1497
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1665..1839
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    1917..1941
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2366..3160
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3247..3835
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3736..3755
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3989..4006
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4021..4161
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4087..4106)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4247..4269
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4505..4699
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4765..5838)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6267..6896)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8197..8221
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8409..8431
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8423..8440
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8424..8440
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8460..8584
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8625..8930)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8727..8746)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9250..9930)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     promoter        complement(join(9984..9990,9991..10008,10009..10014))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(10039..10163)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(10209..10225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10209..10225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10222..10244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10233..10249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10257..10263,10264..10281,10282..10287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10302..10323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
       61 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      121 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      181 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
      241 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      301 tcaaagcaag tggattgatg tgaacatggt ggagcacgac actctcgtct actccaagaa
      361 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
      421 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
      481 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
      541 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
      601 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
      661 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
      721 ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct
      781 ctcgagcttt cgcagatctg tcgatcgacc atggggattg aacaagatgg attgcacgca
      841 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
      901 ggctgctctg atgccgccgt gtttcggctg tcagcgcagg ggcgcccggt tctttttgtc
      961 aagaccgacc tgtccggtgc cctgaatgaa ctccaggacg aggcagcgcg gctatcgtgg
     1021 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     1081 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     1141 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     1201 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     1261 gccggtcttg tcgatcagga tgatctggac gaagaacatc aggggctcgc gccagccgaa
     1321 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacacatggc
     1381 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     1441 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     1501 gaagaacttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     1561 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     1621 ggttcggatc gatcctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
     1681 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
     1741 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     1801 taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta
     1861 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     1921 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     1981 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     2041 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     2101 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     2161 gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg
     2221 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
     2281 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
     2341 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
     2401 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
     2461 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
     2521 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
     2581 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
     2641 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
     2701 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
     2761 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
     2821 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
     2881 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     2941 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     3001 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     3061 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     3121 gaaaataaaa tattatattt tactggatga attgttttag tacctagaat gcatgaccaa
     3181 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3241 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3301 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3361 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3421 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3481 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3541 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3601 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3661 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3721 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3781 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3841 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     3901 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3961 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggg
     4021 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     4081 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     4141 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4201 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtttccgg gagctgcatg
     4261 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     4321 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
     4381 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     4441 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tgggctgtgc gctggccaga
     4501 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     4561 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     4621 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     4681 aatgtacgtg ctatccacag gaaagaggcc ttttcgacct ttttcccctg ctagggcaat
     4741 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     4801 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     4861 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     4921 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
     4981 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
     5041 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
     5101 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
     5161 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
     5221 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
     5281 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
     5341 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
     5401 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
     5461 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
     5521 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
     5581 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
     5641 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
     5701 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
     5761 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
     5821 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
     5881 cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
     5941 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
     6001 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
     6061 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
     6121 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
     6181 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
     6241 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
     6301 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
     6361 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
     6421 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
     6481 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
     6541 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
     6601 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
     6661 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
     6721 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
     6781 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
     6841 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
     6901 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
     6961 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
     7021 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
     7081 gccgcacggt tgagccgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
     7141 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
     7201 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
     7261 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
     7321 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
     7381 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
     7441 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
     7501 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
     7561 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
     7621 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
     7681 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
     7741 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
     7801 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
     7861 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
     7921 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
     7981 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
     8041 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc
     8101 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
     8161 tatccgatta ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc
     8221 aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc tcaagctgct
     8281 ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     8341 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     8401 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccacacttt ttgcaggtga
     8461 ccactttgta caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
     8521 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
     8581 tatggtcgac ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac
     8641 atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc
     8701 ccgataacgg aggccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc
     8761 ccgatatgca ccaccgggta aagttcacgg gggactttat ctgacagcag acgtgcactg
     8821 gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca
     8881 aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagcc
     8941 cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc
     9001 ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt
     9061 tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg
     9121 ctgtcaactg tcactgtaat acgctgcttc atagcatacc tctttttgac atacttcggg
     9181 tatacatatc agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt
     9241 atctggcttt tagtaagccg gatcctctag attacgcccc gcctgccact catcgcagta
     9301 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     9361 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     9421 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     9481 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     9541 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     9601 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     9661 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     9721 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     9781 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     9841 ttgagcgact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     9901 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     9961 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
    10021 ggttcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
    10081 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
    10141 tcagcttttt tgtacaaact tgtcacctgc ttttgatccc cgggtaccga gctcgaattc
    10201 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    10261 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10321 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    10381 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggctagag cagcttgcca
    10441 ac
//
