LOCUS       sequence-182092-       12261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral 
            Replication: BeYDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_221
FEATURES             Location/Qualifiers
     source          1..12261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2746..2766)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4105..4229
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4254..4259,4260..4277,4278..4284)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4338..5018
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5338..5643
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5522..5541
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5684..5808)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7491..7515
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8816..9445
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9874..10947
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11013..11207
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11443..11465)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11551..11691
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11606..11625
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11706..11723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11877..12261,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11957..11976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcaaagtt taacgcgtta
     3721 gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg
     3781 tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga
     3841 atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc
     3901 ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa
     3961 atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt
     4021 atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc
     4081 ctcggtaccg atcaaaagca ggtgacaagt ttgtacaaaa aagctgaacg agaaacgtaa
     4141 aatgatataa atatcaatat attaaattag attttgcata aaaaacagac tacataatac
     4201 tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca ggctttacac
     4261 tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg agattttcag
     4321 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     4381 aatggcatcg taaagaacat tttgaggcat ttcagtcagt cgctcaatgt acctataacc
     4441 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     4501 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     4561 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     4621 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     4681 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     4741 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
     4801 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     4861 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     4921 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     4981 ggcaggcggg gcgtaatcta gaggatccgg cttactaaaa gccagataac agtatgcgta
     5041 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc
     5101 aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcag
     5161 ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc atgcagaatg
     5221 aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg atggctgagg
     5281 tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc tggtgaaatg
     5341 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag
     5401 agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg
     5461 ctgtcagata aagtcccccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg
     5521 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct
     5581 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata
     5641 taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg actggatatg
     5701 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
     5761 atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtca cctgcaaaaa
     5821 gtgtttgatc gccggcggta ccgagtgtac ttcaagtcag tgggaaatca ataaaatgat
     5881 tattttatga atatatttca ttgtgcaagt agatagaaat tacatatgtt acataacaca
     5941 cgaaataaac aaaaaaagac aatccaaaaa caaacacccc aaaaaaaata atcactttag
     6001 ataaactcgt atgaggagag gcacgttcag tgactcgacg attcccgagc aaaaaaagtc
     6061 tccccgtcac acatgtagtg ggtgacgcaa ttatctttaa agtaatcctt ctgttgactt
     6121 gtcattgata acatccagtc ttcgtcagga ttgcaaagaa ttatagaagg gatcccacct
     6181 tttattttct tcttttttcc atatttaggg ttgacagtga aatcagactg gcaacctatt
     6241 aattgcttcc acaatgggac gaacttgaag gggatgtcgt cgatgatatt ataggtggcg
     6301 tgttcatcgt agttggtgaa atcgatggta ccgttccaat agttgtgtcg tccgagactt
     6361 ctagcccagg tggtctttcc ggtacgagtt ggtccgcaga tgtagaggct ggggtgtcgg
     6421 attccattcc ttccattgtc cttgttaaat cggccatcca ttcaaggtca gattgagctt
     6481 gttggtatga gacaggatgt atgtaagtat aagcgtctat gcttacatgg tatagatggg
     6541 tttccctcca ggagtgtaga tcttcgtggc agcgaagatc tgattctgtg aagggcgaca
     6601 catacggttc aggttgtgga gggaataatt tgttggctga atattccagc cattgaagct
     6661 ttgttgccca ttcatgaggg aattcttcct tgatcatgtc aagatattcc tccttagacg
     6721 ttgcagtctg gataatagtt ctccatcgtg cgtcagattt gcgaggagaa accttatgat
     6781 ctcggaaatc tcctctggtt ttaatatctc cgtcctttga tatgtaatca aggacttgtt
     6841 tagagtttct agctggctgg atattagggt gatttccttc aaaatcgaaa aaagaaggat
     6901 ccctaataca aggtttttta tcaagctgga gaagagcatg atagtgggta gtgccatctt
     6961 gatgaagctc agaagcaaca ccaaggaaga aaataagaaa aggtgtgagt ttctcccaga
     7021 gaaactggaa taaatcatct ctttgagatg agcacttggg ataggtaagg aaaacatatt
     7081 tagattggag tctgaagttc ttactagcag aaggcatgtt gttgtgactc cgaggggttg
     7141 cctcaaactc tatcttataa ccggcgtgga ggcatggagg caggggtatt ttggtcattt
     7201 taatagatag tggaaaatga cgtggaattt acttaaagac gaagtctttg cgacaagggg
     7261 gggcccacgc cgaatttaat attaccggcg tggccccccc ttatcgcgag tgctttagca
     7321 cgagcggtcc agatttaaag tagaaaattt cccgcccact agggttaaag gtgttcacac
     7381 tataaaagca tatacgatgt gatggtattt gatggagcgt atattgtatc aggtatttcc
     7441 gttggatacg aattattcgt acgaccctca tagtttaaac tatcagtgtt tgacaggata
     7501 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     7561 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     7621 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     7681 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     7741 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     7801 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     7861 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     7921 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     7981 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     8041 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     8101 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     8161 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     8221 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     8281 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     8341 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     8401 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     8461 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     8521 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     8581 tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg ctgcatgaaa
     8641 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
     8701 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
     8761 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
     8821 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
     8881 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
     8941 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
     9001 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
     9061 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
     9121 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
     9181 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
     9241 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
     9301 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
     9361 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
     9421 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
     9481 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
     9541 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
     9601 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
     9661 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
     9721 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
     9781 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
     9841 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
     9901 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
     9961 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
    10021 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
    10081 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
    10141 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
    10201 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
    10261 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
    10321 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
    10381 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
    10441 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
    10501 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
    10561 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
    10621 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
    10681 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
    10741 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
    10801 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
    10861 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
    10921 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
    10981 gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat tgggaaccca
    11041 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
    11101 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    11161 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    11221 cgcacagccc aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    11281 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    11341 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    11401 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    11461 cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    11521 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    11581 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    11641 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gccctcttcc
    11701 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    11761 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    11821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    11881 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    11941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    12181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    12241 aggattagca gagcgaggta t
//
