LOCUS       sequence-182103-        7395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..7395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             553..1230
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     protein_bind    3049..3060
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4123..4244
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4160..4179)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4214..4233
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4251..4706)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4388..4409)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4600..4619
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4733..4837
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4839..5196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4839..5030
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4862..4882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5047..5182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5231..6025
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5231..6025
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5285..5304)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5895..5914
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6213..6232)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6257..6304
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6633..7221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7122..7141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatgagtgc gattaagcca gacatgaaga tcaaactccg tatggaaggc
      601 aacgtaaacg ggcaccactt tgtgatcgac ggagatggta caggcaagcc ttttgaggga
      661 aaacagagta tggatcttga agtcaaagag ggcggacctc tgccttttgc ctttgatatc
      721 ctgaccactg cattccatta cggcaacagg gtattcgcca aatatccaga caacatacaa
      781 gactatttta agcagtcgtt tcctaagggg tattcgtggg aacgaagctt gactttcgaa
      841 gacgggggca tttgcattgc cagaaacgac ataacaatgg aaggggacac tttctataat
      901 aaagttcgat tttatggtac caactttccc gccaatggtc cagttatgca gaagaagacg
      961 ctgaaatggg agccctccac tgagaaaatg tatgtgcgtg atggagtgct gacgggtgat
     1021 attcatatgg ctttgttgct tgaaggaaat gcccattacc gatgtgactt cagaactact
     1081 tacaaagcta aggagaaggg tgtcaagtta ccaggctacc actttgtgga ccactgcatt
     1141 gagattttaa gccatgacaa agattacaac aaggttaagc tgtatgagca tgctgttgct
     1201 cattctggat tgcctgacaa tgccagacga tccggactca gatctggcag cggtggaggc
     1261 ttggaggagc cggaggccgc gacgcggaca gccgcggccg tggattgcaa agacagaccc
     1321 gggttcccag tgaagaggtt aatacaagcc cgcttgccct tcaaacgcct gaaccttgtt
     1381 cccaaggaga aagttgagga agacacatca ccaaaagcag ctgtggaaag caaagtcccg
     1441 gatctacaat tgtctttggg cacttttgaa agccagtgtc acacaggctc tcacgtaggc
     1501 ttgagtacaa agttggtcgg tggccagggc cccattgata gcttcttgag ggcaacaatc
     1561 aagccagtac cgagtgtggt cattatcgac ctgacggaga actgcagtga cattccagac
     1621 agccctgagg gccacagtga actaagtcct gacactgctg gtgtggtcac cacagtggaa
     1681 ggagctgcca agcagcagga gcactctgca gcagagctgt gtctcctaga gaccccttca
     1741 gacatcactt gtcacatgga agaggagcct gggagtccag gagacccaaa gaggacgggt
     1801 gactgtcagg ctggttcact acagagctgt ccggagctga ccccaggttc taggacatgc
     1861 cccaccaagg agttatcaag ctggagcaag gcaggggatc tcctatttat agagaaagtg
     1921 cctgtggtgg tgctagagga catcctggcc actaagccct ccattgcctc tcttcccatg
     1981 atgtccctgg ataggagtgt cacttcagag agtgaaatac tggagtcctg ccccgaggat
     2041 gactccatac tgagccactc ctccaccaac tcctcttctc caaccagctc tcctgaggga
     2101 ccatccacac ccccagaaca tcggggtggg agaagctccc cctccacccc agcctgcaga
     2161 gttgccaaga actttgtcaa aggctccaca gagaagggca ggagcaaact gcacagagat
     2221 agagaacagc agagggaaga gaaggaaaag ctgagggaag agatacgacg agccaaggag
     2281 gaggcacgga agaagaagga ggaggagaag gagctgaagg aaaaggagcg gagggaaaag
     2341 cgggagaaag atgagaagga gaaggcggag aagcagcggc taaaggagga aaaacgcaag
     2401 gagcggcagg aagctctgga ggctaaactg gaggagaaga ggaagaagga ggaagagaag
     2461 cggttacggg aggaagagaa gcggttacgg gaagaagaga agcgcattaa ggcagaaaag
     2521 gcagagatca ctcgattctt ccagaaaccg aagactccgc aggctcccaa gaccctggcc
     2581 ggttcctgtg gtaagtttgc cccctttgaa atcaaagaac acatggtgct ggctccacgg
     2641 tgccgagctg ccttggatca agacctgtgc gatcagctgg accagctcct gcagcagcag
     2701 agtgtggcaa gcaccttcct cagtgaccta aaaagccggc tgcccttgag atcggggccc
     2761 acccgggtct gtggccatga cacagacatc atgaacaggg atgtggtcat tgtggagagc
     2821 agcaaggtgg acggtgtgtc tgaaaggaag aagtttggcc gcatgaagct tctgcagttc
     2881 tcagagaacc accggccagc atactggggg acgtggaaca agaagacagc catcatccgc
     2941 ccccggaacc cctgggccca ggacaaagac cttcttgact atgaggtaga cagtgacgat
     3001 gagtgggaag aggaggaacc tggggagtct ttgtcccaca gtgaggggga tgaggatgac
     3061 gatgtggggg aagatgagga tgaggacgat ggcttctttg tgccccatgg gtacttatct
     3121 gaagatgaag gcgtgactga ggagtgtgca gaccctgaga accacaaagt acaccagaag
     3181 ctgaaggcca aggagtggga tgagctccta gccaagggca agcgctttcg tgtgctgcag
     3241 cctgtgcacg tgggatgtgt atgggcatca gaggcagcca actgcacgag ttccgacttg
     3301 aagctgctgc agcagttcac tgcatgcctg ctggatgtgg catcccctga tgagccagaa
     3361 cccggggcct ccaggaggga aaagagggac cagcatatcc tggcccagtt gcttccgctg
     3421 ctacatggca atgtgaatgg gagcaaggtg atcatccatg aattccagga gcaatgccgc
     3481 aggggcctgc tgacccttcc aagtcccacc ccacacctgc agatgcccaa cttggaggat
     3541 gctgttgctg tgccatccaa ggccaggctg aagcgcctta tctctgagaa ctctgcctat
     3601 gagaagcggc ccaacttccg tatgtgctgg tatgtgcacc cagaggtgct caagagcttt
     3661 ggccaggagt gcctgccagt gccctgccag tggacctaca tcaccaccat gccctcagca
     3721 cccagggaag acagtggcag tgcttctact gaggggcctg gccagagcac acctatgcta
     3781 ctcaagcgga agccagcggc caccatgtgc atcacgcagt ttatgaagaa gcggcgctat
     3841 gatggacagg ttgggtctgg ggacatggat ggcttccagg cagacacaga agaggatgaa
     3901 gaggatgaca cagactgtat gatcatagat gtgccagatg ttgggagtga cgtgtcagaa
     3961 gctcccatac ctgctcccac actttgcaag tgaggatcca ccggatctag ataactgatc
     4021 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4081 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
     4261 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     4321 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     4381 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     4441 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     4501 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     4561 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     4621 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     4681 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
     4741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4801 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
     4861 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     4921 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     4981 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5041 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5101 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5161 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
     5221 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     5281 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5341 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5401 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5461 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5521 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5581 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5641 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5701 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     5761 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     5821 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5881 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     5941 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6001 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6061 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6121 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6181 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
     6241 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
     6301 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     6361 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     6421 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
     6481 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6541 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7261 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7321 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
     7381 atatatggag ttccg
//
