LOCUS       sequence-182117-       12895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral 
            Replication: ToLCV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_212
FEATURES             Location/Qualifiers
     source          1..12895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(2919..3595)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2962..2982)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3786..3807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3822..3827,3828..3845,3846..3852)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3860..3876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3865..3887
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3884..3900
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3884..3900
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4262..4386
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4411..4416,4417..4434,4435..4441)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4495..5175
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5495..5800
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5679..5698
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5841..5965)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(6002..6254)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    8125..8149
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9450..10079
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10508..11581
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11647..11841
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12077..12099)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12185..12325
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12240..12259
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12340..12357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12511..12895,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12591..12610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
     2581 atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccgga tctgcgaaag
     2881 ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct ctccaaatga
     2941 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc
     3001 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     3061 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     3121 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     3181 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     3241 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     3301 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc aatccacttg
     3361 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     3421 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     3481 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     3541 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     3601 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     3661 caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg
     3721 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3781 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     3841 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     3901 atgattacga attcgagctc aaagtttaac gcgtcgatta agttgacttg gtcaatcggt
     3961 gtctctcaac tctctctatg tatcggtgta ttggagtcct atatatatgg agactctaat
     4021 ggcataaatg taaatatgga actttaaatc aaaaccctta cgctccaaaa agcggccatc
     4081 cgtataatat taccggatgg ccgcgatttt ttttttggac cccacaacgc aataactgac
     4141 aaagacatgc ggaccaatca cctgacgcgc tcaaagctta attgttttgt gttttctatt
     4201 taaacttggg caccaagtat tgtagttcgt actacaagtt tgtaccgatc aaaagcaggt
     4261 gacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt
     4321 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc
     4381 actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat
     4441 gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag
     4501 aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt
     4561 gaggcatttc agtcagtcgc tcaatgtacc tataaccaga ccgttcagct ggatattacg
     4621 gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt
     4681 cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg
     4741 gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt
     4801 tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa
     4861 gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg
     4921 tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat
     4981 atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag
     5041 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     5101 agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag
     5161 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
     5221 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     5281 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     5341 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     5401 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     5461 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     5521 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     5581 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga
     5641 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     5701 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     5761 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     5821 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     5881 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     5941 gttcagcttt cttgtacaaa gtggtcacct gcaaaaagtg tttgatcgcc ggcggtacaa
     6001 agtggtagta acatagatga caccgcgcgc gataatttat cctagtttgc gcgctatatt
     6061 ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa aacccatctc
     6121 ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt caacagaaat
     6181 tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact ttattgccaa
     6241 atgtttgaac gatcggggaa attcctaata aatattaaat tttattatat gcgaactgtc
     6301 tacaaatgtt gtttgcgtca atgcatccca tagtacataa ttcactgctc taattacatt
     6361 attcaaacta attacaccca aattattaag aaatttcaac acttgtgttt taaataccct
     6421 taagaaacga ccagtcggag gctgtgaggt catccagatt cggtaggtta ggaaacattt
     6481 gtgtatcccc aacactttcc tcaggttgtg attgaactgt atccggacgg tgatgatgtc
     6541 ttcgttcatc acgaagggcc ggttttggtg ctctgttatc ttgaaatata ggggattttg
     6601 aatctcccag ataaacacgc cattctctgc ttgagctgca gtgatgagtt cccctgtgcg
     6661 tgaatccatg gtcgtggcag gttattgcta tgaagtaaga acagccacac ggtagatcaa
     6721 ctcgtcgacg tctggtcccc ttcttggcta gcctgtgctg tactttgatt ggtacctgag
     6781 tagagtgggc cttcgagggt gacgaaggtc gcatttttta aagcccagtt ttttagtgca
     6841 gaattctttt cctcatccaa gtattcttta tagctggagt tgggtcctgg attgcagagg
     6901 aaggtagtgg gaattccgcc tttaatttga actggcttcc cgtactttgt atttgattgc
     6961 cagtcccttt gggcccccat gaattcttta aagtgcttta ggtagtgggg gtctacgtca
     7021 tcaatgacgt tgtaccaggc atcattactg taaacctttg ggctaaggtc tagatgacca
     7081 cacaaataat tatgtggtcc caatgatctg gcccacatag tcttccccgt cctactgtca
     7141 ccctcaatta ctatacttat gggtctcaga ggccgcgcag cgggactgca gacgttctcg
     7201 gcagcccact cttcaagttc ttctggaact tggtcgaaag aagaagataa aaaaggtgaa
     7261 acataaacat ccattggagg tgtaaaaatc ctatctaaat tagaatttaa attgtgaaat
     7321 tgtaaaacaa aatctttggg agctttctcc ctcaatatat taagggcctg agctttggac
     7381 cctgaattga ttgcctcggc atatgcgtcg ttggcagatt ggcaacctcc tctagctgat
     7441 cgtccatcga cttggaaaac tccatgatca atgacgtctc cgtctttttc catgtaggat
     7501 ttgacatcgc ttgagctctt agctccctga atgttcggat ggaaatgtgc tgacctggtt
     7561 ggggatgtga ggtcgaagaa tctattgttt ttgcactgga actttccttc gaactggatg
     7621 agcacatgca agtgaggagt cccatcttcg tgaagctctc tgcaaattct aataaatttt
     7681 ttgaaagtgg gtgtttggag atttaataat ttgggaaagt gctcttcttt agttagagag
     7741 cacttgggat aagtgagaaa ataatttttg gcatttattt taaagcgatt gggggctgcc
     7801 atgttgactt ggtcaatcgg tgtctctcaa ctctctctat gtatcggtgt attggagtcc
     7861 tatatatatg gagactctaa tggcataaat gtaaatatgg aactttaaat caaaaccctt
     7921 acgctccaaa aagcggccat ccgtataata ttaccggatg gccgcgattt tttttttgga
     7981 ccccacaacg caataactga caaagacatg cggaccaatc acctgacgcg ctcaaagctt
     8041 aattgttttg tgttttctat ttaaacttgg gcaccaagta ttgtagttcg tacttggggc
     8101 cctcgagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa
     8161 gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc
     8221 atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc
     8281 cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga
     8341 catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt
     8401 tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta
     8461 cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga
     8521 ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc
     8581 cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct
     8641 acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga
     8701 cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga
     8761 gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat
     8821 tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa
     8881 ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc
     8941 ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt
     9001 gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc
     9061 caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc
     9121 cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg
     9181 tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg
     9241 cccgcgcacg gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag
     9301 ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg
     9361 tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga
     9421 gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag
     9481 gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg
     9541 ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc
     9601 gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga
     9661 aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg
     9721 ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg
     9781 acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg
     9841 gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg
     9901 aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc
     9961 gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg
    10021 gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag
    10081 ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag
    10141 cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg
    10201 ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca
    10261 aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag
    10321 caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag
    10381 aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag
    10441 gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg
    10501 aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga
    10561 tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga
    10621 agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca
    10681 accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga
    10741 ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt
    10801 ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct
    10861 tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta
    10921 cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg
    10981 gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg
    11041 ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa
    11101 caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt
    11161 atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc
    11221 ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa
    11281 cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt
    11341 tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac
    11401 gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa
    11461 gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg
    11521 cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta
    11581 atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct
    11641 ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc
    11701 gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat
    11761 aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa
    11821 aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gcccaagagc tgcaaaaagc
    11881 gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc
    11941 cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc
    12001 cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg
    12061 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga aacggtcaca gcttgtctgt
    12121 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
    12181 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
    12241 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
    12301 cgtaaggaga aaataccgca tcaggccctc ttccgcttcc tcgctcactg actcgctgcg
    12361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    12421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    12481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    12541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    12601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    12661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    12721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    12781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    12841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtat
//
