LOCUS       sequence-182118-       12679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral 
            Replication: ToLCV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_222
FEATURES             Location/Qualifiers
     source          1..12679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2746..2766)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4046..4170
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4195..4200,4201..4218,4219..4225)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4279..4959
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5279..5584
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5463..5482
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5625..5749)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(5786..6038)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7909..7933
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9234..9863
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10292..11365
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11431..11625
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11861..11883)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11969..12109
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12024..12043
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12124..12141)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12295..12679,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12375..12394)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcaaagtt taacgcgtcg
     3721 attaagttga cttggtcaat cggtgtctct caactctctc tatgtatcgg tgtattggag
     3781 tcctatatat atggagactc taatggcata aatgtaaata tggaacttta aatcaaaacc
     3841 cttacgctcc aaaaagcggc catccgtata atattaccgg atggccgcga tttttttttt
     3901 ggaccccaca acgcaataac tgacaaagac atgcggacca atcacctgac gcgctcaaag
     3961 cttaattgtt ttgtgttttc tatttaaact tgggcaccaa gtattgtagt tcgtactaca
     4021 agtttgtacc gatcaaaagc aggtgacaag tttgtacaaa aaagctgaac gagaaacgta
     4081 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
     4141 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
     4201 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
     4261 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     4321 caatggcatc gtaaagaaca ttttgaggca tttcagtcag tcgctcaatg tacctataac
     4381 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     4441 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     4501 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     4561 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     4621 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     4681 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     4741 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     4801 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     4861 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     4921 tggcaggcgg ggcgtaatct agaggatccg gcttactaaa agccagataa cagtatgcgt
     4981 atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc cgaagtatgt
     5041 caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg acagctatca
     5101 gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac catgcagaat
     5161 gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg gatggctgag
     5221 gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg ctggtgaaat
     5281 gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg tggatgtaca
     5341 gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca gtgcacgtct
     5401 gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg atgaaagctg
     5461 gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg aagaagtggc
     5521 tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt tctggggaat
     5581 ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt gactggatat
     5641 gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt aatatattga
     5701 tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtc acctgcaaaa
     5761 agtgtttgat cgccggcggt acaaagtggt agtaacatag atgacaccgc gcgcgataat
     5821 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
     5881 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
     5941 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
     6001 aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcct aataaatatt
     6061 aaattttatt atatgcgaac tgtctacaaa tgttgtttgc gtcaatgcat cccatagtac
     6121 ataattcact gctctaatta cattattcaa actaattaca cccaaattat taagaaattt
     6181 caacacttgt gttttaaata cccttaagaa acgaccagtc ggaggctgtg aggtcatcca
     6241 gattcggtag gttaggaaac atttgtgtat ccccaacact ttcctcaggt tgtgattgaa
     6301 ctgtatccgg acggtgatga tgtcttcgtt catcacgaag ggccggtttt ggtgctctgt
     6361 tatcttgaaa tataggggat tttgaatctc ccagataaac acgccattct ctgcttgagc
     6421 tgcagtgatg agttcccctg tgcgtgaatc catggtcgtg gcaggttatt gctatgaagt
     6481 aagaacagcc acacggtaga tcaactcgtc gacgtctggt ccccttcttg gctagcctgt
     6541 gctgtacttt gattggtacc tgagtagagt gggccttcga gggtgacgaa ggtcgcattt
     6601 tttaaagccc agttttttag tgcagaattc ttttcctcat ccaagtattc tttatagctg
     6661 gagttgggtc ctggattgca gaggaaggta gtgggaattc cgcctttaat ttgaactggc
     6721 ttcccgtact ttgtatttga ttgccagtcc ctttgggccc ccatgaattc tttaaagtgc
     6781 tttaggtagt gggggtctac gtcatcaatg acgttgtacc aggcatcatt actgtaaacc
     6841 tttgggctaa ggtctagatg accacacaaa taattatgtg gtcccaatga tctggcccac
     6901 atagtcttcc ccgtcctact gtcaccctca attactatac ttatgggtct cagaggccgc
     6961 gcagcgggac tgcagacgtt ctcggcagcc cactcttcaa gttcttctgg aacttggtcg
     7021 aaagaagaag ataaaaaagg tgaaacataa acatccattg gaggtgtaaa aatcctatct
     7081 aaattagaat ttaaattgtg aaattgtaaa acaaaatctt tgggagcttt ctccctcaat
     7141 atattaaggg cctgagcttt ggaccctgaa ttgattgcct cggcatatgc gtcgttggca
     7201 gattggcaac ctcctctagc tgatcgtcca tcgacttgga aaactccatg atcaatgacg
     7261 tctccgtctt tttccatgta ggatttgaca tcgcttgagc tcttagctcc ctgaatgttc
     7321 ggatggaaat gtgctgacct ggttggggat gtgaggtcga agaatctatt gtttttgcac
     7381 tggaactttc cttcgaactg gatgagcaca tgcaagtgag gagtcccatc ttcgtgaagc
     7441 tctctgcaaa ttctaataaa ttttttgaaa gtgggtgttt ggagatttaa taatttggga
     7501 aagtgctctt ctttagttag agagcacttg ggataagtga gaaaataatt tttggcattt
     7561 attttaaagc gattgggggc tgccatgttg acttggtcaa tcggtgtctc tcaactctct
     7621 ctatgtatcg gtgtattgga gtcctatata tatggagact ctaatggcat aaatgtaaat
     7681 atggaacttt aaatcaaaac ccttacgctc caaaaagcgg ccatccgtat aatattaccg
     7741 gatggccgcg attttttttt tggaccccac aacgcaataa ctgacaaaga catgcggacc
     7801 aatcacctga cgcgctcaaa gcttaattgt tttgtgtttt ctatttaaac ttgggcacca
     7861 agtattgtag ttcgtacttg gggccctcga gtttaaacta tcagtgtttg acaggatata
     7921 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
     7981 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     8041 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     8101 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     8161 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     8221 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     8281 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     8341 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     8401 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     8461 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     8521 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     8581 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     8641 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     8701 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     8761 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     8821 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     8881 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     8941 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     9001 gcggccgggt acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc
     9061 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     9121 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     9181 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     9241 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     9301 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     9361 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     9421 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     9481 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     9541 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     9601 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     9661 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     9721 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     9781 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     9841 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     9901 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     9961 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
    10021 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
    10081 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
    10141 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
    10201 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgcctgccgg ccctgcaatg
    10261 gcactggaac ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt
    10321 acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc
    10381 ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga
    10441 tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc
    10501 gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg
    10561 cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc
    10621 tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc
    10681 cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga
    10741 atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca
    10801 cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct
    10861 ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc
    10921 caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat
    10981 cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta
    11041 ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt
    11101 gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc
    11161 agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa
    11221 gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt
    11281 gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga
    11341 gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc
    11401 aggggaaaaa ggtcgaaaag gcctctttcc tgtggatagc acgtacattg ggaacccaaa
    11461 gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg
    11521 gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc
    11581 tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg
    11641 cacagcccaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc
    11701 cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg
    11761 caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca
    11821 aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    11881 cggaaacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    11941 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
    12001 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
    12061 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc cctcttccgc
    12121 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    12181 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    12241 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    12301 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    12361 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    12421 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    12481 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    12541 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    12601 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    12661 gattagcaga gcgaggtat
//
