LOCUS       sequence-182120-       11947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral 
            Replication: BeYDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11947)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 11947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_231
FEATURES             Location/Qualifiers
     source          1..11947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2389..3065)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2432..2452)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3256..3277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3292..3297,3298..3315,3316..3322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3330..3346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3335..3357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3354..3370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3354..3370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3791..3915
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3940..3945,3946..3963,3964..3970)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4024..4704
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5024..5329
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5208..5227
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5370..5494)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    7177..7201
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8502..9131
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9560..10633
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10699..10893
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11129..11151)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11237..11377
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11292..11311
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11392..11409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11563..11947,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11643..11662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtcga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcatggtaga ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     2401 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     2461 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     2521 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     2581 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     2641 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     2701 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     2761 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     2821 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     2881 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     2941 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     3001 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     3061 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     3121 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
     3181 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3241 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3301 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3361 cagctatgac atgattacga attcgagctc aaagtttaac gcgttagcag aaggcatgtt
     3421 gttgtgactc cgaggggttg cctcaaactc tatcttataa ccggcgtgga ggcatggagg
     3481 caggggtatt ttggtcattt taatagatag tggaaaatga cgtggaattt acttaaagac
     3541 gaagtctttg cgacaagggg gggcccacgc cgaatttaat attaccggcg tggccccccc
     3601 ttatcgcgag tgctttagca cgagcggtcc agatttaaag tagaaaattt cccgcccact
     3661 agggttaaag gtgttcacac tataaaagca tatacgatgt gatggtattt gatggagcgt
     3721 atattgtatc aggtatttcc gttggatacg aattattcgt acgaccctcg gtaccgatca
     3781 aaagcaggtg acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat
     3841 caatatatta aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca
     3901 tatccagtca ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc
     3961 tcgtataatg tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct
     4021 aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa
     4081 gaacattttg aggcatttca gtcagtcgct caatgtacct ataaccagac cgttcagctg
     4141 gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt
     4201 attcacattc ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac
     4261 ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact
     4321 gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata
     4381 tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt
     4441 gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac
     4501 gtggccaata tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa
     4561 ggcgacaagg tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc
     4621 catgtcggca gaatgcttaa tgaattacaa cagtactgcg atgagtggca ggcggggcgt
     4681 aatctagagg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
     4741 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtatgc
     4801 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
     4861 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
     4921 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
     4981 gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt ttaaggttta
     5041 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
     5101 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
     5161 cccccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
     5221 cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg
     5281 cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc
     5341 ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt
     5401 attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt
     5461 acgtttctcg ttcagctttc ttgtacaaag tggtcacctg caaaaagtgt ttgatcgccg
     5521 gcggtaccga gtgtacttca agtcagtggg aaatcaataa aatgattatt ttatgaatat
     5581 atttcattgt gcaagtagat agaaattaca tatgttacat aacacacgaa ataaacaaaa
     5641 aaagacaatc caaaaacaaa caccccaaaa aaaataatca ctttagataa actcgtatga
     5701 ggagaggcac gttcagtgac tcgacgattc ccgagcaaaa aaagtctccc cgtcacacat
     5761 gtagtgggtg acgcaattat ctttaaagta atccttctgt tgacttgtca ttgataacat
     5821 ccagtcttcg tcaggattgc aaagaattat agaagggatc ccacctttta ttttcttctt
     5881 ttttccatat ttagggttga cagtgaaatc agactggcaa cctattaatt gcttccacaa
     5941 tgggacgaac ttgaagggga tgtcgtcgat gatattatag gtggcgtgtt catcgtagtt
     6001 ggtgaaatcg atggtaccgt tccaatagtt gtgtcgtccg agacttctag cccaggtggt
     6061 ctttccggta cgagttggtc cgcagatgta gaggctgggg tgtcggattc cattccttcc
     6121 attgtccttg ttaaatcggc catccattca aggtcagatt gagcttgttg gtatgagaca
     6181 ggatgtatgt aagtataagc gtctatgctt acatggtata gatgggtttc cctccaggag
     6241 tgtagatctt cgtggcagcg aagatctgat tctgtgaagg gcgacacata cggttcaggt
     6301 tgtggaggga ataatttgtt ggctgaatat tccagccatt gaagctttgt tgcccattca
     6361 tgagggaatt cttccttgat catgtcaaga tattcctcct tagacgttgc agtctggata
     6421 atagttctcc atcgtgcgtc agatttgcga ggagaaacct tatgatctcg gaaatctcct
     6481 ctggttttaa tatctccgtc ctttgatatg taatcaagga cttgtttaga gtttctagct
     6541 ggctggatat tagggtgatt tccttcaaaa tcgaaaaaag aaggatccct aatacaaggt
     6601 tttttatcaa gctggagaag agcatgatag tgggtagtgc catcttgatg aagctcagaa
     6661 gcaacaccaa ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa ctggaataaa
     6721 tcatctcttt gagatgagca cttgggatag gtaaggaaaa catatttaga ttggagtctg
     6781 aagttcttac tagcagaagg catgttgttg tgactccgag gggttgcctc aaactctatc
     6841 ttataaccgg cgtggaggca tggaggcagg ggtattttgg tcattttaat agatagtgga
     6901 aaatgacgtg gaatttactt aaagacgaag tctttgcgac aagggggggc ccacgccgaa
     6961 tttaatatta ccggcgtggc ccccccttat cgcgagtgct ttagcacgag cggtccagat
     7021 ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt tcacactata aaagcatata
     7081 cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt atttccgttg gatacgaatt
     7141 attcgtacga ccctcatagt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     7201 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     7261 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac
     7321 tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
     7381 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
     7441 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
     7501 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
     7561 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
     7621 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
     7681 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
     7741 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
     7801 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
     7861 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
     7921 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
     7981 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
     8041 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
     8101 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
     8161 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
     8221 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
     8281 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
     8341 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
     8401 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     8461 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     8521 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     8581 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     8641 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     8701 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     8761 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     8821 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     8881 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     8941 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     9001 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     9061 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     9121 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     9181 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     9241 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     9301 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     9361 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     9421 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     9481 gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc actggaaccc
     9541 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     9601 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     9661 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     9721 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     9781 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     9841 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     9901 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     9961 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
    10021 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
    10081 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
    10141 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
    10201 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
    10261 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
    10321 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
    10381 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
    10441 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
    10501 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
    10561 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
    10621 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
    10681 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
    10741 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
    10801 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
    10861 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
    10921 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
    10981 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
    11041 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
    11101 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
    11161 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
    11221 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
    11281 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
    11341 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
    11401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    11461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    11521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    11581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    11641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    11701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11941 gaggtat
//
