LOCUS       sequence-182121-       12365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral 
            Replication: ToLCV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12365)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_232
FEATURES             Location/Qualifiers
     source          1..12365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(2389..3065)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2432..2452)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3256..3277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3292..3297,3298..3315,3316..3322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3330..3346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3335..3357
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3354..3370
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3354..3370
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3732..3856
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3881..3886,3887..3904,3905..3911)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3965..4645
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             4965..5270
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5149..5168
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5311..5435)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(5472..5724)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7595..7619
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8920..9549
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9978..11051
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11117..11311
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11547..11569)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11655..11795
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11710..11729
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11810..11827)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11981..12365,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12061..12080)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtcga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcatggtaga ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct
     2401 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     2461 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     2521 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     2581 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     2641 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     2701 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     2761 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc
     2821 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     2881 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     2941 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     3001 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     3061 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     3121 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
     3181 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     3241 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     3301 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     3361 cagctatgac atgattacga attcgagctc aaagtttaac gcgtcgatta agttgacttg
     3421 gtcaatcggt gtctctcaac tctctctatg tatcggtgta ttggagtcct atatatatgg
     3481 agactctaat ggcataaatg taaatatgga actttaaatc aaaaccctta cgctccaaaa
     3541 agcggccatc cgtataatat taccggatgg ccgcgatttt ttttttggac cccacaacgc
     3601 aataactgac aaagacatgc ggaccaatca cctgacgcgc tcaaagctta attgttttgt
     3661 gttttctatt taaacttggg caccaagtat tgtagttcgt actacaagtt tgtaccgatc
     3721 aaaagcaggt gacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     3781 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     3841 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     3901 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     3961 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     4021 agaacatttt gaggcatttc agtcagtcgc tcaatgtacc tataaccaga ccgttcagct
     4081 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     4141 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     4201 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     4261 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     4321 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     4381 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     4441 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     4501 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     4561 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc aggcggggcg
     4621 taatctagag gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat
     4681 ttttgcggta taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg
     4741 ctatgaagca gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat
     4801 atatgatgtc aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct
     4861 gcgtgccgaa cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat
     4921 tgaaatgaac ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt
     4981 acacctataa aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg
     5041 acacgcccgg gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag
     5101 tcccccgtga actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca
     5161 ccgatatggc cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc
     5221 gcgaaaatga catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct
     5281 cccttataca cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag
     5341 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     5401 tacgtttctc gttcagcttt cttgtacaaa gtggtcacct gcaaaaagtg tttgatcgcc
     5461 ggcggtacaa agtggtagta acatagatga caccgcgcgc gataatttat cctagtttgc
     5521 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
     5581 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
     5641 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
     5701 ttattgccaa atgtttgaac gatcggggaa attcctaata aatattaaat tttattatat
     5761 gcgaactgtc tacaaatgtt gtttgcgtca atgcatccca tagtacataa ttcactgctc
     5821 taattacatt attcaaacta attacaccca aattattaag aaatttcaac acttgtgttt
     5881 taaataccct taagaaacga ccagtcggag gctgtgaggt catccagatt cggtaggtta
     5941 ggaaacattt gtgtatcccc aacactttcc tcaggttgtg attgaactgt atccggacgg
     6001 tgatgatgtc ttcgttcatc acgaagggcc ggttttggtg ctctgttatc ttgaaatata
     6061 ggggattttg aatctcccag ataaacacgc cattctctgc ttgagctgca gtgatgagtt
     6121 cccctgtgcg tgaatccatg gtcgtggcag gttattgcta tgaagtaaga acagccacac
     6181 ggtagatcaa ctcgtcgacg tctggtcccc ttcttggcta gcctgtgctg tactttgatt
     6241 ggtacctgag tagagtgggc cttcgagggt gacgaaggtc gcatttttta aagcccagtt
     6301 ttttagtgca gaattctttt cctcatccaa gtattcttta tagctggagt tgggtcctgg
     6361 attgcagagg aaggtagtgg gaattccgcc tttaatttga actggcttcc cgtactttgt
     6421 atttgattgc cagtcccttt gggcccccat gaattcttta aagtgcttta ggtagtgggg
     6481 gtctacgtca tcaatgacgt tgtaccaggc atcattactg taaacctttg ggctaaggtc
     6541 tagatgacca cacaaataat tatgtggtcc caatgatctg gcccacatag tcttccccgt
     6601 cctactgtca ccctcaatta ctatacttat gggtctcaga ggccgcgcag cgggactgca
     6661 gacgttctcg gcagcccact cttcaagttc ttctggaact tggtcgaaag aagaagataa
     6721 aaaaggtgaa acataaacat ccattggagg tgtaaaaatc ctatctaaat tagaatttaa
     6781 attgtgaaat tgtaaaacaa aatctttggg agctttctcc ctcaatatat taagggcctg
     6841 agctttggac cctgaattga ttgcctcggc atatgcgtcg ttggcagatt ggcaacctcc
     6901 tctagctgat cgtccatcga cttggaaaac tccatgatca atgacgtctc cgtctttttc
     6961 catgtaggat ttgacatcgc ttgagctctt agctccctga atgttcggat ggaaatgtgc
     7021 tgacctggtt ggggatgtga ggtcgaagaa tctattgttt ttgcactgga actttccttc
     7081 gaactggatg agcacatgca agtgaggagt cccatcttcg tgaagctctc tgcaaattct
     7141 aataaatttt ttgaaagtgg gtgtttggag atttaataat ttgggaaagt gctcttcttt
     7201 agttagagag cacttgggat aagtgagaaa ataatttttg gcatttattt taaagcgatt
     7261 gggggctgcc atgttgactt ggtcaatcgg tgtctctcaa ctctctctat gtatcggtgt
     7321 attggagtcc tatatatatg gagactctaa tggcataaat gtaaatatgg aactttaaat
     7381 caaaaccctt acgctccaaa aagcggccat ccgtataata ttaccggatg gccgcgattt
     7441 tttttttgga ccccacaacg caataactga caaagacatg cggaccaatc acctgacgcg
     7501 ctcaaagctt aattgttttg tgttttctat ttaaacttgg gcaccaagta ttgtagttcg
     7561 tacttggggc cctcgagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     7621 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     7681 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt
     7741 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt
     7801 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt
     7861 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc
     7921 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca
     7981 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca
     8041 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc
     8101 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca
     8161 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta
     8221 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt
     8281 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg
     8341 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga
     8401 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac
     8461 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc
     8521 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc
     8581 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca
     8641 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt
     8701 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc
     8761 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg
     8821 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat
     8881 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt
     8941 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa
     9001 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg
     9061 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac
     9121 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg
     9181 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca
     9241 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag
     9301 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc
     9361 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt
     9421 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca
     9481 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa
     9541 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg
     9601 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag
     9661 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg
     9721 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag
     9781 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg
     9841 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc
     9901 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc
     9961 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg
    10021 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca
    10081 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag
    10141 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg
    10201 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca
    10261 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc
    10321 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg
    10381 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat
    10441 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac
    10501 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca
    10561 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc
    10621 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa
    10681 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag
    10741 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca
    10801 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt
    10861 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca
    10921 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg
    10981 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg
    11041 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc
    11101 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga
    11161 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag
    11221 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta
    11281 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc
    11341 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc
    11401 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc
    11461 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc
    11521 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
    11581 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
    11641 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
    11701 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
    11761 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
    11821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12361 ggtat
//
