LOCUS       sequence-182123-       11169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression vector of 53BP1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Escribano-Diaz C, Orthwein A, Fradet-Turcotte A, Xing M, Young JT, 
            Tkac J, Cook MA, Rosebrock AP, Munro M, Canny MD, Xu D, Durocher D
  TITLE     A cell cycle-dependent regulatory circuit composed of 53BP1-RIF1 and
            BRCA1-CtIP controls DNA repair pathway choice.
  JOURNAL   Mol Cell. 2013 Mar 7;49(5):872-83. doi: 
            10.1016/j.molcel.2013.01.001. Epub 2013 Jan 17.
  PUBMED    23333306
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.01.001"; journalName: "Mol Cell"; date: 
            "2013-03-7- 7"; volume: "49"; issue: "5"; pages: "872-83"
COMMENT     Sequence Label: pcDNA5-FRT/T0-Flag-53BP1
FEATURES             Location/Qualifiers
     source          1..11169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(22..39)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(193..781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(273..292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(952..1743,1744..1812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1575..1594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1813..1917)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1992..2011)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2183..2562
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2563..2766
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2717..2737
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2768..2786
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2789..2807
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2817..2841
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2941..2964
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(9069..9086)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9075..9299
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9432..9451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    9583..9630
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             9638..10660
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    10790..10911
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10827..10846)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10881..10900
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10960..10976)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10960..10976)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10973..10995)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10984..11000
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11008..11014,11015..11032,11033..11038))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11053..11074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
       61 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      121 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      181 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      241 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      301 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      361 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      421 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      481 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      541 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      601 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      661 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      721 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      781 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      841 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
      901 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
      961 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1021 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1081 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1141 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1201 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1261 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1321 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1381 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1441 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1501 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1561 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1621 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     1681 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     1741 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     1801 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     1861 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     1921 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg
     1981 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct
     2041 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc
     2101 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
     2161 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
     2221 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2281 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2341 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2401 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2461 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2521 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2581 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2641 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2701 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2761 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
     2821 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
     2881 agacaccggg accgatccag cctccggact ctagcgttta aacttaagct tggtaccatg
     2941 gactacaaag acgatgacga taaagccgcc agtttaaacg gcgcgccatt aattaaggat
     3001 ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca ctggcggccg
     3061 ctcgagatgg accctactgg aagtcagttg gattcagatt tctctcagca agatactcct
     3121 tgcctgataa ttgaagattc tcagcctgaa agccaggttc tagaggatga ttctggttct
     3181 cacttcagta tgctatctcg acaccttcct aatctccaga cgcacaaaga aaatcctgtg
     3241 ttggatgttg tgtccaatcc tgaacaaaca gctggagaag aacgaggaga cggtaatagt
     3301 gggttcaatg aacatttgaa agaaaacaag gttgcagacc ctgtggattc ttctaacttg
     3361 gacacatgtg gttccatcag tcaggtcatt gagcagttac ctcagccaaa caggacaagc
     3421 agtgttctgg gaatgtcagt ggaatctgct cctgctgtgg aggaagagaa gggagaagag
     3481 ttggaacaga aggagaaaga gaaggaagaa gatacttcag gcaatactac acattccctt
     3541 ggtgctgaag atactgcctc atcacagttg ggttttgggg ttctggaact ctcccagagc
     3601 caggatgttg aggaaaatac tgtgccatat gaagtggaca aagagcagct acaatcagta
     3661 accaccaact ctggttatac caggctgtct gatgtggatg ctaatactgc aattaagcat
     3721 gaagaacagt ccaacgaaga tatccccata gcagaacagt ccagcaagga catccctgtg
     3781 acagcacagc ccagtaagga tgtacatgtt gtaaaagagc aaaatccacc acctgcaagg
     3841 tcagaggaca tgccttttag ccccaaagca tctgttgctg ctatggaagc aaaagaacag
     3901 ttgtctgcac aagaacttat ggaaagtgga ctgcagattc agaagtcacc agagcctgag
     3961 gttttgtcaa ctcaggaaga cttgtttgac cagagcaata aaacagtatc ttctgatggt
     4021 tgctctactc cttcaaggga ggaaggtggg tgttctttgg cttccactcc tgccaccact
     4081 ctgcatctcc tgcagctctc tggtcagagg tcccttgttc aggacagtct ttccacgaat
     4141 tcttcagatc ttgttgctcc ttctcctgat gctttccgat ctactccttt tatcgttcct
     4201 agcagtccca cagagcaaga agggagacaa gataagccaa tggacacgtc agtgttatct
     4261 gaagaaggag gagagccttt tcagaagaaa cttcaaagtg gtgaaccagt ggagttagaa
     4321 aacccccctc tcctgcctga gtccactgta tcaccacaag cctcaacacc aatatctcag
     4381 agcacaccag tcttccctcc tgggtcactt cctatcccat cccagcctca gttttctcat
     4441 gacattttta ttccttcccc aagtctggaa gaacaatcaa atgatgggaa gaaagatgga
     4501 gatatgcata gttcatcttt gacagttgag tgttctaaaa cttcagagat tgaaccaaag
     4561 aattcccctg aggatcttgg gctatctttg acaggggatt cttgcaagtt gatgctttct
     4621 acaagtgaat atagtcagtc cccaaagatg gagagcttga gttctcacag aattgatgaa
     4681 gatggagaaa acacacagat tgaggatacg gaacccatgt ctccagttct caattctaaa
     4741 tttgttcctg ctgaaaatga tagtatcctg atgaatccag cacaggatgg tgaagtacaa
     4801 ctgagtcaga atgatgacaa aacaaaggga gatgatacag acaccaggga tgacattagt
     4861 attttagcca ctggttgcaa gggcagagaa gaaacggtag cagaagatgt ttgtattgat
     4921 ctcacttgtg attcggggag tcaggcagtt ccgtcaccag ctactcgatc tgaggcactt
     4981 tctagtgtgt tagatcagga ggaagctatg gaaattaaag aacaccatcc agaggagggg
     5041 tcttcagggt ctgaggtgga agaaatccct gagacacctt gtgaaagtca aggagaggaa
     5101 ctcaaagaag aaaatatgga gagtgttccg ttgcaccttt ctctgactga aactcagtcc
     5161 caagggttgt gtcttcaaaa ggaaatgcca aaaaaagaat gctcagaagc tatggaagtt
     5221 gaaaccagtg tgattagtat tgattcccct caaaagttgg caatacttga ccaagaattg
     5281 gaacataagg aacaggaagc ttgggaagaa gctacttcag aggactccag tgttgtcatt
     5341 gtagatgtga aagagccatc tcccagagtt gatgtttctt gtgaaccttt ggagggagtg
     5401 gagaagtgct cagattccca gtcatgggag gatattgctc cagaaataga accatgtgct
     5461 gagaatagat tagacaccaa ggaagaaaag agtgtagaat atgaaggaga tctgaaatca
     5521 gggactgcag aaacagaacc tgtagagcaa gattcttcac agccttcctt acctttagta
     5581 cgcgctgacg acccgcttag acttgaccag gagttgcagc agccccaaac tcaggagaaa
     5641 acaagtaatt cattaacaga agactcaaaa atggctaatg caaagcagct aagctcagat
     5701 gcagaggccc agaagctggg gaagccctct gcccatgcct cacaaagctt ctgtgaaagt
     5761 tctagtgaaa ccccatttca tttcactttg cctaaagaag gtgatatcat cccaccattg
     5821 actggtgcaa ccccacctct tattgggcac ctaaaattgg agcccaagag acacagtact
     5881 cctattggta ttagcaacta tccagaaagc accatagcaa ccagtgatgt catgtctgaa
     5941 agcatggtgg agacccatga tcccatactt gggagtggaa aaggggattc tggggctgcc
     6001 ccagacgtgg atgataaatt atgtctaaga atgaaactgg ttagtcctga gactgaggcg
     6061 agtgaagagt ctttgcagtt caacctggaa aagcctgcaa ctggtgaaag aaaaaatgga
     6121 tctactgctg ttgctgagtc tgttgccagt ccccagaaga ccatgtctgt gttgagctgt
     6181 atctgtgaag ccaggcaaga gaatgaggct cgaagtgagg atccccccac cacacccatc
     6241 agggggaact tgctccactt tccaagttct caaggagaag aggagaaaga aaaattggag
     6301 ggtgaccata caatcaggca gagtcaacag cctatgaagc ccattagtcc tgtcaaggac
     6361 cctgtttctc ctgcttccca gaagatggtc atacaagggc catccagtcc tcaaggagag
     6421 gcaatggtga cagatgtgct agaagaccag aaagaaggac ggagtactaa taaggaaaat
     6481 cctagtaagg ccttgattga aaggcccagc caaaataaca taggaatcca aaccatggag
     6541 tgttccttga gggtcccaga aactgtttca gcagcaaccc agactataaa gaatgtgtgt
     6601 gagcagggga ccagtacagt ggaccagaac tttggaaagc aagatgccac agttcagact
     6661 gagaggggga gtggtgagaa accagtcagt gctcctgggg atgatacaga gtcgctccat
     6721 agccagggag aagaagagtt tgatatgcct cagcctccac atggccatgt cttacatcgt
     6781 cacatgagaa caatccggga agtacgcaca cttgtcactc gtgtcattac agatgtgtat
     6841 tatgtggatg gaacagaagt agaaagaaaa gtaactgagg agactgaaga gccaattgta
     6901 gagtgtcagg agtgtgaaac tgaagtttcc ccttcacaga ctgggggctc ctcaggtgac
     6961 ctgggggata tcagctcctt ctcctccaag gcatccagct tacaccgcac atcaagtggg
     7021 acaagtctct cagctatgca cagcagtgga agctcaggga aaggagccgg accactcaga
     7081 gggaaaacca gcgggacaga acccgcagat tttgccttac ccagctcccg aggaggccca
     7141 ggaaaactga gtcctagaaa aggggtcagt cagacaggga cgccagtgtg tgaggaggat
     7201 ggtgatgcag gccttggcat cagacaggga gggaaggctc cagtcacgcc tcgtgggcgt
     7261 gggcgaaggg gccgcccacc ttctcggacc actggaacca gagaaacagc tgtgcctggc
     7321 cccttgggca tagaggacat ttcacctaac ttgtcaccag atgataaatc cttcagccgt
     7381 gtcgtgcccc gagtgccaga ctccaccaga cgaacagatg tgggtgctgg tgctttgcgt
     7441 cgtagtgact ctccagaaat tcctttccag gctgctgctg gcccttctga tggcttagat
     7501 gcctcctctc caggaaatag ctttgtaggg ctccgtgttg tagccaagtg gtcatccaat
     7561 ggctactttt actctgggaa aatcacacga gatgtcggag ctgggaagta taaattgctc
     7621 tttgatgatg ggtacgaatg tgatgtgttg ggcaaagaca ttctgttatg tgaccccatc
     7681 ccgctggaca ctgaagtgac ggccctctcg gaggatgagt atttcagtgc aggagtggtg
     7741 aaaggacata ggaaggagtc tggggaactg tactacagca ttgaaaaaga aggccaaaga
     7801 aagtggtata agcgaatggc tgtcatcctg tccttggagc aaggaaacag actgagagag
     7861 cagtatgggc ttggccccta tgaagcagta acacctctta caaaggcagc agatatcagc
     7921 ttagacaatt tggtggaagg gaagcggaaa cggcgcagta acgtcagctc cccagccacc
     7981 cctactgcct ccagtagcag cagcacaacc cctacccgaa agatcacaga aagtcctcgt
     8041 gcctccatgg gagttctctc aggcaaaaga aaacttatca cttctgaaga ggaacggtcc
     8101 cctgccaagc gaggtcgcaa gtctgccaca gtaaaacctg gtgcagtagg ggcaggagag
     8161 tttgtgagcc cctgtgagag tggagacaac accggtgaac cctctgccct ggaagagcag
     8221 agagggcctt tgcctctcaa caagaccttg tttctgggct acgcatttct ccttaccatg
     8281 gccacaacca gtgacaagtt ggccagccgc tccaaactgc cagatggtcc tacaggaagc
     8341 agtgaagaag aggaggaatt tttggaaatt cctcctttca acaagcagta tacagaatcc
     8401 cagcttcgag caggagctgg ctatatcctt gaagatttca atgaagccca gtgtaacaca
     8461 gcttaccagt gtcttctaat tgcggatcag cattgtcgaa cccggaagta cttcctgtgc
     8521 cttgccagtg ggattccttg tgtgtctcat gtctgggtcc atgatagttg ccatgccaac
     8581 cagctccaga actaccgtaa ttatctgttg ccagctgggt acagccttga ggagcaaaga
     8641 attctggact ggcaaccccg tgaaaatcct ttccagaatc tgaaggtact cttggtatca
     8701 gaccaacagc agaacttcct ggagctctgg tctgagatcc tcatgactgg tggtgcagcc
     8761 tctgtgaagc agcaccattc aagtgcccat aacaaagata ttgctttagg ggtatttgat
     8821 gtggtggtga cggacccctc atgcccagcc tcggtgctga agtgtgctga agcattgcag
     8881 ctgcctgtgg tgtcacaaga gtgggtgatc cagtgcctca ttgttgggga gagaattgga
     8941 ttcaagcagc atccaaaata taaacacgat tatgtttctc actaaagata cttggtctta
     9001 ctggttttat tccctgctat cgtggagatt gtgttttaac caggttttcg tttaaacccg
     9061 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     9121 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     9181 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     9241 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     9301 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     9361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag
     9601 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac
     9661 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     9721 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     9781 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     9841 ggccgcgctc ccgattccgg aagtgcttga cattggggag ttcagcgaga gcctgaccta
     9901 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     9961 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
    10021 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
    10081 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
    10141 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
    10201 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
    10261 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
    10321 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
    10381 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
    10441 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
    10501 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
    10561 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
    10621 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga
    10681 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    10741 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
    10801 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    10861 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    10921 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
    10981 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    11041 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    11101 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11161 cggtttgcg
//
