LOCUS       sequence-182131-        1724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP1071-2
FEATURES             Location/Qualifiers
     source          1..1724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(112..140)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     837..857
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             898..939
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             1689..1709
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cggtacccgg ggatcgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
       61 tacaaggatg acgatgacaa ggtgagtaaa ggagaagaac ttttcactgg agttgtccca
      121 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      181 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      241 cctgttccat ggccaacact tgtcactact ctgacctacg gcgtgcagtg cttcagccgg
      301 taccccgacc acatgaagca gcatgacttt ttcaaggtaa gtttaaacat atatatacta
      361 actaaccctg attatttaaa ttttcagagt gccatgcccg aaggttatgt acaggaaaga
      421 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      481 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      541 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      601 caaaaggtaa gtttaaacag ttcggtacta actaaccata catatttaaa ttttcagaat
      661 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      721 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      781 tacctgtcca cacaatctaa gctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      841 cttcttgagt ttgtaacagc tgctgggatt acactcggca tggacgagct gtacaagtcc
      901 ggattcgcca acgagctcgg accacgtctc atgggaaagc caccaaagcc atctgccaag
      961 ggagccaaga aggccgccaa gaccgtcgtt gccaagccaa aggacggaaa gaagagacgt
     1021 catgcccgca aggaatcgta ctccgtctac atctaccgtg ttctcaagca agttcaccca
     1081 gacaccggag tctcctccaa ggccatgtct atcatgaact ccttcgtcaa cgatgtattc
     1141 gaacgcatcg cttcggaagc ttcccgtctt gctcattaca acaaacgctc aacgatctca
     1201 tcccgcgaaa ttcaaaccgc tgtccgtttg attctcccag gagaacttgc caagcacgcc
     1261 gtgtctgagg gaaccaaggc cgtcaccaag tacacttcca gcaagtaaac tagtgataaa
     1321 tgcaagatcc tttcaagcat tcccttcttc tctatcactc ttctttcttt ttgtcaaaaa
     1381 attctctcgc taatttattt gcttttttaa tgttattatt ttatgacttt ttatagtcac
     1441 tgaaaagttt gcatctgagt gaagtgaatg ctatcaaaat gtgattctgt ctgatgtact
     1501 ttcacaatct ctcttcaatt ccattttgaa gtgctttaaa cccgaaaggt tgagaaaaat
     1561 gcgagcgctc aaatatttgt attgtgttcg ttgagtgacc caacaaaaag aggaaacttt
     1621 attgtgccgc caagaaatgc ccgggggatc ggtggagctc caccggtggc ggccgctcta
     1681 gaactagtga gaacctctac ttccaaggag atcctctaga gtcg
//
