LOCUS       sequence-182135-       12652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter,
            also expresses mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Yoshimatsu T, D'Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, 
            Raible DW, Wong RO
  TITLE     Presynaptic partner selection during retinal circuit reassembly 
            varies with timing of neuronal regeneration in vivo.
  JOURNAL   Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590.
  PUBMED    26838932
REFERENCE   2  (bases 1 to 12652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-3"; volume: "7:10590. doi"; pages: " 
            10.1038/ncomms10590"
COMMENT     Sequence Label: pCx55.5-TrpR
FEATURES             Location/Qualifiers
     source          1..12652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(561..572,573..735)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1108..1124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1144..1164
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     1166..1185
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1166..1184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5816..5840
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6212..6553
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    complement(6624..6648)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6650..6784
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6847..6867)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6875..6891)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6907..7041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7177..7887)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7455..7474)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7724..7742
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     gap             9686..9696
                     /estimated_length=11
     misc_feature    join(10182..10369,10370..10381)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10415..10431)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10415..10431)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10468..10488)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10468..10486)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10507..10523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10520..10542)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10531..10547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10555..10561,10562..10579,10580..10585))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10600..10621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10738..10755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10909..11497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10989..11008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11668..12459,12460..12528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12291..12310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12529..12633)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaatg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat
      781 taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa
      841 ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt
      901 aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg
      961 aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta
     1021 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
     1081 gaaacacagg ccagatgggc cctcgagcag gaaacagcta tgaccatgat tacgccaagc
     1141 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtacaag
     1201 cgctaccgga ctcagatctc gagagatctt tccaacagca cacaataata cacttgatat
     1261 atatggcttt gagtaatcaa tgtacatttc tgaaatttac agaaatatcc gacccctgat
     1321 gtgacctgta aagggcattt gaggatgaaa agagggactt ctgctgacca atcacatctg
     1381 ttaggtaaca gtctagcagt actttccact acagagcaga attggggttg tgatttaagt
     1441 caatgtgtga tgcactaacc ccaggaataa aacattaatc taaatttggg ctattgatga
     1501 taattgactg atgtctgggt ctgtgttttc aactaactgt tatttacttt agccatcatt
     1561 gctaacagac aagtgttgat cggccaccaa taggacattt gctcctgcaa acagataatt
     1621 tgtctatatc aatttcagaa atgcatctcc tttcgtaata tttacaaaaa ttttagccct
     1681 actgtgaaat taatttattt tcagatattt cccacatgct gaaatagcat cttctttttt
     1741 aacaatagtt ttaataaata atttcttttg tctttgctat gataattgca cattatacca
     1801 gctattttgc aagatattag tattcagttt aaagtgcaat ttaaaagctt aactagtagg
     1861 ttaattggag tacatatagg tttcataata ttaagcacta aacattagag gtttatgggg
     1921 cctgttgaaa atatattttt aaataatatt ttacaaacaa actaatagaa ataagaattt
     1981 ctccagaata ataacatagg aaatactgtt taataaatgt gttaaacatc actagggaaa
     2041 tatctgaaaa agaattaaat gtttatctgt gtaaatattt atatactgat gtagcatgac
     2101 ctgtaataat tgttctgtca tgctatatcc atacagtgta gggatgtatg ttgagactac
     2161 tataaatgtt taatcagacg cagattaaaa ttagtaagtg atcttgcggc tcagtaacag
     2221 aggtcacttc taaatccaac agtgtttaaa tacataaaca ttccctttta ttaatatgtt
     2281 tgtgtaagat catgatataa ttatcttaac aagcataaat tgcattcaag ctataatatc
     2341 ctaatatatt ctaatatcct tatattgaat ctgtaaaaaa aatgttaatt aaccctagag
     2401 ttcaagaaaa tgagtttttt tttggacatt ttagttgttt gaaataatat aatgcaaaag
     2461 aaataatata taaagaaata agtctgcaga ataacagatt atacacgggt aggttacaag
     2521 gttttgtacc ataatataca actccaaccc agacgatttg tcactttaaa ctattcgaaa
     2581 aagtcccttt gggaacttaa ggttaaaagt tgttggaaga cacatcaagg tctcataatg
     2641 caattcaaaa gcataaataa acattataaa atcttgaatt attcacagat gattccttgg
     2701 acttacaaat aattttatgt taagtggttg taaacaatgt attttgcctg aattaaacaa
     2761 agcaattaag ttgaatatta ttgcatttaa ttagtttgtt taagttcaac acacataaat
     2821 tgtttgcaac aacttttcag atattgtttt ttcttcagtg tatggtgaac tgctaattta
     2881 tggatatttt ttaacagtgg atgcattaaa aaatttttag ttcttcaaat taagtgtcaa
     2941 agtttgtgta tgatcatgct ataattatct caacaaaaat aaattgcatt taagctatac
     3001 tatcccacat gcactgtaca aaatatctgt taattaacag tattttgtga ttcacaagtg
     3061 tttccccttt atttatggtt gtgaattgca ttaatggaac tttgatctct gtcgactttt
     3121 gatgttgaaa attcacctac aatttaacaa aatgactttt attgacattt taacaaagca
     3181 taagaaatta tatgtagaga aatatatctg taaaataaag gaatgcatac gggcagcttg
     3241 gttttgtacc atattatacg acaccatcaa cccggacaat ttgtcacttt aaactattcg
     3301 aaatgttagt aagtcaattt tttttaactt gaaggataaa agtcaaaaac atcaaggtct
     3361 cataatgcaa tttaaaagca tgaataatct tttttacggt tgtaaattgg attatgggac
     3421 gttggtctct gttctgttga cttttgatgt tgaactttca acattacagt ttaaagttta
     3481 cagtgtctta aataacagaa aatgcatggc agctttttaa acaagctgtt tgtactgcaa
     3541 gatacagcac caaccctgac aatatgtcac tttaatctat ttaaaatgtt cataaaagtc
     3601 actttgtcaa acatttcaag gttaaaagat caagatatgc aattcaaaat caaaaataaa
     3661 cggggaaaaa tatcaaataa aaacataaat cacaaaaaat ggaaaactgc aaatttccgg
     3721 atatattttt acattgtggt ctgttttgta gaataccaag ctttaaatta attcattaaa
     3781 gcatctccag tctaaatctg actatgcagc tcatttatta tattaaatat aggacttgac
     3841 atagccccta aatatgtcca agccttgaat gaaagcaacc aaccaacagc atagttagtg
     3901 agtgtaatgg ggttgcacca ttgatttctt ttaaatggaa taaagtatca ccacaactag
     3961 cagagacaac ttaattacac tgaatccagc agagtatgaa atcggacatg accgattgat
     4021 tcttttctga actatcgact gctaaaagta gtgccaacat gtggccaaat gccattagaa
     4081 acttagcttt gataataaca actcccttca gctttctggt ttttaagtat taatcaaatt
     4141 aaatgtgtaa atgttttagt tttatgtctt aaaaaaagaa ccaataggtc ttcagttctg
     4201 ttgaaaacta ataggcctag tgtgtgtgtt tatctcaagt tggcacccat gttgggctag
     4261 atttgcagat cagctgaaaa aatcgttctg caatgggtgt taactagcaa tcaaccacgc
     4321 aagactgtct aacattactt taatcgatag cgggtgcaca atagcatgag gaatcaagtg
     4381 tacccaagcg taaattcata cagcctacag aagtttttat gttttatttt atcattgcag
     4441 ggttttactt acgcatggta tacagtggca gtgttagcaa gctgaatcaa ttcagttttc
     4501 ccttcatgtt gatagtggag cactgattat tgacgctctc acatacagaa gcagcgcgct
     4561 tgccaacaaa gccctgcgca ctcggaaacc ttcttttggt gccaacaaag aggaaagagg
     4621 gggtcacaaa agtttaggtg atgtgctgcg tcagatgtgc agggcaccaa cgatttttaa
     4681 atattttggt ggcctcgacg cgcctctatg acccgcgggg atttaaagca gcggcagact
     4741 gtccatactg ctgtaaggta aatgggcaca ttgatttctc ctgcatgact ataagttggc
     4801 acctagacga cctgcttgtt atgtgtgtag cccagacctt taattataag cgggattgca
     4861 tgagtctagc tttttgtagc tagtgtgtag atgcaggatg ggcttgttac aatgaagatt
     4921 tacttgattt ccccaacttt tgtttaaggt acgttgatgg tttatagttg atttatttgc
     4981 tttctattaa agataaagtc gcatggagtt agaactgtaa acacagcaat tgatttcact
     5041 tattagcacc acatgcaatg tcaatttttt gtgacccatt aatagatgtt aattaagctt
     5101 gctgtaattg tatttcagta caaacatgct tgtatttaag gatgtttatg acctaatttc
     5161 ttggaacatg ttaaaatatt ttaacaaata tatatttatt atctttaacg ttatattatt
     5221 attatagtta ttgttattaa tttacatatt tatctgtgac tacacatata tatatatata
     5281 tatatattta acacagctca gattgcagaa agaatattac aattgtggtt ctatttaagt
     5341 attttaaacc atgttagata cacagctaat atgtgggtgg tgctttattt taaaatggta
     5401 attaataact aatttatcta gaaaaatggt ggaaagtttt tttttacatg ttggtctgcc
     5461 attaatctaa attttgttgg agagattggg tttttttata cttgactttc agcttataaa
     5521 atgttttgaa acatagggtt gagacttttg tctggattta ttgtttgcat atttgtgtga
     5581 ggttactgtt ggaaaacatt gattttaaat taaagctctc aaacatttaa ataacagttt
     5641 tatactctgg ttgatttaat ttcaatatat tttcatattt aaaagcaatt tcccatggtc
     5701 taggttcatg tttctttctt cttattttgt ctttacagac acaaaaaagc ccgagtaaag
     5761 gatccaccgg tcactagttc tagagcggcc gccaccgcgg acaggtttaa acaattcaag
     5821 tttgtacaaa aaagcaggct ggaccatggc acccaagaag aagaggaagg cccaacaatc
     5881 accctattca gcagcgatgg cagaacagcg tcaccaggag tggttacgtt ttgtggacct
     5941 gcttaagaat gcctaccaaa acgatctcca tttaccgttg ttaaacctga tgctgacgcc
     6001 agatgagcgc aaagcgttgg ggactcgcgt gcgtattgtc gaagagctgt tgcgcggcga
     6061 aatgagccag cgtgagttaa aaaatgaact cggcgcaggc atcgcgacga ttacgcgtgg
     6121 atctaacagc ctgaaagccg cgcccgtcga gctgcgccag tggctggaag aggtgttgct
     6181 gaaaagcgat gggtcgacgc tagccggtgg cgccaatttt aatcaaagtg ggaatattgc
     6241 tgatagctca ttgtccttca ctttcactaa cagtagcaac ggtccgaacc tcataacaac
     6301 tcaaacaaat tctcaagcgc tttcacaacc aattgcctcc tctaacgttc atgataactt
     6361 catgaataat gaaatcacgg ctagtaaaat tgatgatggt aataattcaa aaccactgtc
     6421 acctggttgg acggaccaaa ctgcgtataa cgcgtttgga atcactacag ggatgtttaa
     6481 taccactaca atggatgatg tatataacta tctattcgat gatgaagata ccccaccaaa
     6541 cccaaaaaaa gagggtctag aattcctcga cggatcgtag atccagacat gataagatac
     6601 attgatgagt ttggacaaac cacacccagc tttcttgtac aaagtggggg atccagacat
     6661 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     6721 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     6781 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     6841 tttttccaac tttattatac atagttgata attcactggc cgtcgtttta cggtaccatc
     6901 gatgatgatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6961 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     7021 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     7081 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat
     7141 tatgatcctc tagatcagat ccagagtcgc ggccgcttac ttgtacagct cgtccatgcc
     7201 gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt
     7261 gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt
     7321 cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag
     7381 cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc
     7441 ctcccagccc atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg
     7501 cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac
     7561 cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag
     7621 cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa
     7681 ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt
     7741 ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg
     7801 gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat
     7861 gttatcctcc tcgcccttgc tcaccatcgg tacctgtctg ctttgctgtt tggtggtctg
     7921 ggctcctggg tcactggctt actaatggag tctttatgta tgaggactct tatcaattgt
     7981 tcttctataa aggtctgcag tgtttctgtt cgtcccctac atggacaccc agagcctcct
     8041 aaatacagga gccctgataa ctgcacaagt gctcagattc cagcagggtg gaaaatgaga
     8101 taaagtgtgc agatggggag ggggacgtga atgagagaag cttgagctcg agatctgtcc
     8161 cgatctttct cttctgtgct gtcaatttag agtagttttt gaggtaattt gggtgcattc
     8221 tctgtaaaaa caacaaataa atatttatat tgtatgcaat aaatgtaaca attacacata
     8281 cactctctga ttacagcatt aaagggttag ttcacccaaa aatgaaaaga tgtcattaat
     8341 gactcaccct catgtcgttc caagcccgta agacctccgt tcatcttcag aacacagttt
     8401 aagatatttt agatttagtc cgagagcttt ctgtgcctcc attgagaatg tatgtacggt
     8461 atactgtcca tgtccagaaa ggtaataaaa acatcaaagt agtccatgtg acatcagtgg
     8521 gttagttaga attttttgaa gcatcgaata cattttggtc caaaaataac aaaacctacg
     8581 actttattcg gcattgtatt ctcttccggg tctgttgtca atccgcgttc acgacttcgc
     8641 agtgacgcta caatgctgaa taaagtcgta ggttttgtta tttttggacc aaaatgtatt
     8701 ttcgatgctt caaataattc tacctaaccc actgatgtca catggactac tttgatgttt
     8761 ttattacctt tctggacatg gacagtatac cgtacataca ttttcagtgg agggacagaa
     8821 agctctcgga ctaaatctaa aatatcttaa actgtgttcc gaagatgaac ggaggtgtta
     8881 cgggcttgga acgacatgag ggtgagtcat taatgacatc ttttcatttt tgggtgaact
     8941 aaccctttaa taaaactgac atttgcagcc aattgcacac tttataatgt taaatacttg
     9001 ttgattttag aaatacagta gcatttagtg acaatcaaac attaatttta tttctgatta
     9061 tattgacaaa aacacaggac tgtaaggctg acatgacagg gtgaattgat ttcattacat
     9121 tagttttgat ggtaacactt ttacaataag gttcgttagt taactacatt agttaacatg
     9181 aactaataat gaactgcacg tatacatgtt aatttcaaca tttactaata ctttatatct
     9241 tgttaacatt agttaatgca ctgtgaacta acatgaacaa acaatgaaca tctgtatttc
     9301 tattaactaa cgttaacaaa gattaacaga tacagtaaca aatgtattgc tcatggttat
     9361 ttaatgttag ttaatacatg aactaatcaa cctcattgta aagtgttacc gttttgacaa
     9421 tacaacatgt ttggaaaaaa atttctcgct tggttttctt gtgttttgca tgaattcaaa
     9481 cacaatgaga gcacggaatg ataatgatta ttcaaagctg caataggcgc caaatttccc
     9541 gtggacctaa cgttaccaat tacaaatgca ttttagcatt ttattacgtg agccttatat
     9601 tgtcaatcgc atgcattagt gaaaataggc ctactacaca tataaaaggc gcgccatcaa
     9661 cgtcaaaaca tgcacaccca aaaaannnnn nnnnnnaaaa aaaaactatc agtaaaacaa
     9721 aatacttaat gtacttacct caatatgctt ccttaggttt gatggcgaac ttttgaaggc
     9781 cgatatttct ttattaagcg ggagacagag gacacatttc atcttgaatg aatctttatt
     9841 tacaccactt aaagaaaaga attcgcgaag atacggccac gggtgctctt gatcctgtgg
     9901 ctgattttgg actgtgctgc tcgcagctgc tgatgaatca catacttcct ccattttctt
     9961 ccactgattg actgttataa tttccctaat ttccaggtca aggtgctgtg cattgtggta
    10021 atagatgtga catgacgtca cttccaaagg accaatgaac atgtctgacc aatttcatat
    10081 aatgtgaaaa cgattttcat aggcagaata aataacattt aaattaaact gggcatcagc
    10141 gcaattcaat tggtttggta atagcaaggg aaaatagaat gaagtgatct ccaaaaaata
    10201 agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa gtactttttt ttctaaaaaa
    10261 atgtaattaa gtaaaagtaa aagtattgat ttttaattgt actcaagtaa agtaaaaatc
    10321 cccaaaaata atacttaagt acagtaatca agtaaaatta ctcaagtact ttacacctct
    10381 ggttcttgac cccctacctt cagcaagccc agcagatcca ctagttctag agcggccgcc
    10441 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    10501 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10561 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10621 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10681 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10741 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10801 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10861 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10921 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10981 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    11041 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    11101 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11161 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11221 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11281 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11341 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11401 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11461 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11521 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11581 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11641 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11701 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11761 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11821 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11881 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11941 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    12001 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    12061 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12121 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12181 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12241 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12301 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12361 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12421 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12481 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12541 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12601 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tg
//
