LOCUS       sequence-182136-       13641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral 
            Replication: WDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13641)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_253
FEATURES             Location/Qualifiers
     source          1..13641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1827..2852)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4828..4833,4834..4851,4852..4858)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4866..4882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4871..4893
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4890..4906
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4890..4906
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5364..5488
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5513..5518,5519..5536,5537..5543)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5597..6277
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             6597..6902
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6781..6800
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6943..7067)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8871..8895
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10196..10825
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11254..12327
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12393..12587
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12823..12845)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12931..13071
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12986..13005
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13086..13103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(13257..13641,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13337..13356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
     1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
     1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
     1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
     2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
     2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
     2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
     2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
     2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
     2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
     2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
     2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
     2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag
     2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
     2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
     2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
     2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg
     2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac
     2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat
     2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa
     3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa
     3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg
     3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc
     3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta
     3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat
     3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata
     3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca
     3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga
     3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt
     3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca
     3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc
     3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac
     3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca
     3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca
     3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg
     3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc
     3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat
     4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga
     4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt
     4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag
     4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg
     4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt
     4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat
     4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc
     4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt
     4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt
     4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta
     4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt
     4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag
     4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc
     4921 gagtaaggta gtgaacagaa gtccggcagg tccttagcga aaaaacgggg tgtgccagaa
     4981 aactctatcc tctaccctgc gtggaggtgt gaattctgca cactgcaaat gcaatgtgtc
     5041 caatgcttta tatagggcag gttttggcgg gagaacaggg ccctagtgtt cccacggtag
     5101 cgtagcgaat cgtgtgggcc ctgttcggtg tgcggtcggg gggcctccac gcgggttata
     5161 atattacccc gcgtggtggc ccccgacgcg cactcggctt ttcgtgagtg cgcggaggct
     5221 tttggaccac atcttttctg atcactttcg tggaagatgt tgatttatca cacttttgac
     5281 ggggaaatct gtgccatgcc ttagcttata aggaagtgcg tggtagccca tctcgacaag
     5341 tttgtaccga tcaaaagcag gtgacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
     5401 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
     5461 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
     5521 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga gattttcagg
     5581 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     5641 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtc gctcaatgta cctataacca
     5701 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     5761 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     5821 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     5881 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     5941 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     6001 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     6061 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     6121 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     6181 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     6241 gcaggcgggg cgtaatctag aggatccggc ttactaaaag ccagataaca gtatgcgtat
     6301 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     6361 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     6421 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     6481 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     6541 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     6601 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     6661 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     6721 tgtcagataa agtcccccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     6781 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     6841 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     6901 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     6961 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     7021 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtcac ctgcaaaaag
     7081 tgtttgatcg ccggcggtac aaagtggtta aaataatatt ttatttatct catgtcattc
     7141 gattacagag gctcggctac gagcaaagac aaaccaaata taacaaacaa caacccttac
     7201 acaatgacat cggaaaacga aatacaacac cctgagatat tacatttata gaaactgtac
     7261 gccgtccgcg ctaggacagt cactgcgaag cagtgacgtc ttcgccggag gcgaacgagt
     7321 agttgatgaa cgtctcgcct tcatacatgt agtgaacaac agtgttagag tacatgtaat
     7381 ccgactgttc gggagtcata tccttgagcc aatcttcgtc tggattaact aaaatgatgc
     7441 aaggtattcc accccgtatg acctttcgct taccatattt tggattgacc gtgaagtcac
     7501 gctgagcccc gacgaagcac ttccagttgg gtgtgaactt gaatggaatg tcgtcgatga
     7561 tattatactt ggcgttgacg tcatatgttg tgaaatcaac tagactgtta taataattgt
     7621 gtgtccctag agaccttgcc caggaagtct ttcctgttct ggttggcccg cagatgtaga
     7681 tggacttatg cctccccggt gactcctgga ataatcgtcc atccactcta agtcagattg
     7741 cgcttgatcc gcaggagtgg aagtacaaag gatataggat tcgaggctta cggagtagag
     7801 atgttcattt ttccagcttt caatggtctc atggcaaatg agtgattcgg ttggaaactc
     7861 aggtgtgtaa gtggcaactg ggtcaggaaa tagatggcgt gccgtgtact cgaagtcttt
     7921 gagacggata gaccattcaa acggaaaacg attgcaaacc atgctgagga attcctcgcg
     7981 agaggaacta gattcaatga tctgtttcat atccgcatca cggtctttac gacctggagt
     8041 tgaaacagcc acgaatgttc cccactcagc tgtgtttaca tcggagtcaa cctccttcgt
     8101 gatgtaatca cgaacttggt tgcagtcttt ggcagcttgt atatttggat ggaatatgga
     8161 gaatggagat gtatccatac ggaggtttaa ggcattggga ttggtgatgg aagcacgaag
     8221 cttgttctgc acgagaacgt gcagatgtgg tgatccatct tcgtggagct ctctaacagc
     8281 agcgatgtag aggggctcat atttgttcaa gagagtgcga agtgaatcca aggcgtactg
     8341 tggctcaagg gtacattgag gatatgttag aaagaggtac ttggaataga cacggaacct
     8401 gggtgcagat gaagaggcca tggtagtgaa cagaagtccg gcaggtcctt agcgaaaaaa
     8461 cggggtgtgc cagaaaactc tatcctctac cctgcgtgga ggtgtgaatt ctgcacactg
     8521 caaatgcaat gtgtccaatg ctttatatag ggcaggtttt ggcgggagaa cagggcccta
     8581 gtgttcccac ggtagcgtag cgaatcgtgt gggccctgtt cggtgtgcgg tcggggggcc
     8641 tccacgcggg ttataatatt accccgcgtg gtggcccccg acgcgcactc ggcttttcgt
     8701 gagtgcgcgg aggcttttgg accacatctt ttctgatcac tttcgtggaa gatgttgatt
     8761 tatcacactt ttgacgggga aatctgtgcc atgccttagc ttataaggaa gtgcgtggta
     8821 gcccatctcg gggccctcga ttcgacgttc ctgtttaaac tatcagtgtt tgacaggata
     8881 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     8941 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca gggttcccct
     9001 cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg cttctgacgt
     9061 tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac gcgacaggct
     9121 gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat
     9181 acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc tggcctgctg
     9241 ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc cgaactgcac
     9301 gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg cgaccgcccg
     9361 gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt gaccaggcta
     9421 gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc
     9481 ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc ggccggccgc
     9541 atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat catcgaccgc
     9601 acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc ccgccctacc
     9661 ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg
     9721 aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc
     9781 agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga ggacgcattg
     9841 accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca agcatgaaac
     9901 cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag gcggagatga
     9961 tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg ctgcatgaaa
    10021 tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag
    10081 aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg
    10141 cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa
    10201 ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct
    10261 agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg
    10321 cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga
    10381 ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga
    10441 cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct
    10501 gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt
    10561 taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc
    10621 gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc
    10681 cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac
    10741 aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc
    10801 tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca
    10861 aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc
    10921 ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc
    10981 aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac
    11041 atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc
    11101 taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat
    11161 gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc ggccctgcaa
    11221 tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg
    11281 gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc
    11341 gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct
    11401 gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag
    11461 ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc
    11521 cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga
    11581 gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg
    11641 gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc
    11701 gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca
    11761 cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac
    11821 ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag
    11881 gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag
    11941 atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg
    12001 taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt
    12061 ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag
    12121 gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc
    12181 aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat
    12241 ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc
    12301 gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta
    12361 gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat tgggaaccca
    12421 aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac
    12481 cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac
    12541 tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag
    12601 cgcacagccc aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc
    12661 gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca
    12721 ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat
    12781 caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
    12841 cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
    12901 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
    12961 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    13021 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gccctcttcc
    13081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    13141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    13201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    13261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    13321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    13381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    13441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    13501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    13561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    13621 aggattagca gagcgaggta t
//
