LOCUS       sequence-182139-       13133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral 
            Replication: WDV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 13133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_263
FEATURES             Location/Qualifiers
     source          1..13133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1793..2344)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     protein_bind    4284..4305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4320..4325,4326..4343,4344..4350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4358..4374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4363..4385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4382..4398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4382..4398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4856..4980
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5005..5010,5011..5028,5029..5035)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5089..5769
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             6089..6394
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6273..6292
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6435..6559)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    8363..8387
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9688..10317
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10746..11819
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11885..12079
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12315..12337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12423..12563
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12478..12497
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12578..12595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12749..13133,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12829..12848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct
     1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac
     1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat
     1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca
     1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca
     2041 gtcccgtccg ctggtggcgg gggctgacgt acacggtgga ctcggccgtc cagtcgtagg
     2101 cgttgcgtgc cttccaggga cccgcgtagg cgatgccggc gacctcgccg tccacctcgg
     2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt
     2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc
     2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc
     2341 tcataagctt ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca
     2401 gaactcatca caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc
     2461 acatcattag tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg
     2521 catgatcatc aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc
     2581 atgcatatct atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta
     2641 taggaacagc agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca
     2701 gtttcaattt aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat
     2761 gtagcagcat atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg
     2821 tgtgctattc agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata
     2881 atcatcttaa tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg
     2941 atcttattct actctgatct acagcaatca gataacgacc taaccttgca catggcaaca
     3001 aaacaatcga tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat
     3061 agatgcacgt acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag
     3121 gcagcgaccc agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca
     3181 cgattcccgg acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca
     3241 gcggaaccga tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca
     3301 cgcggatcta gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag
     3361 aaccaccaaa ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt
     3421 accttgaagc gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc
     3481 cgggggttgt gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg
     3541 ccctgtattt atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc
     3601 cccacccgtg ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc
     3661 aatttccgcg atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat
     3721 ttgtagacgc cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac
     3781 aatggcttaa cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg
     3841 caatgggatt ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg
     3901 acatggtctt cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt
     3961 tagaagttaa gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa
     4021 actttagaga ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt
     4081 cggacggtgt gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg
     4141 gtggggctca agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt
     4201 tagttggctt cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     4261 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     4321 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     4381 acaggaaaca gctatgacat gattacgaat tcgagtaagg tagtgaacag aagtccggca
     4441 ggtccttagc gaaaaaacgg ggtgtgccag aaaactctat cctctaccct gcgtggaggt
     4501 gtgaattctg cacactgcaa atgcaatgtg tccaatgctt tatatagggc aggttttggc
     4561 gggagaacag ggccctagtg ttcccacggt agcgtagcga atcgtgtggg ccctgttcgg
     4621 tgtgcggtcg gggggcctcc acgcgggtta taatattacc ccgcgtggtg gcccccgacg
     4681 cgcactcggc ttttcgtgag tgcgcggagg cttttggacc acatcttttc tgatcacttt
     4741 cgtggaagat gttgatttat cacacttttg acggggaaat ctgtgccatg ccttagctta
     4801 taaggaagtg cgtggtagcc catctcgaca agtttgtacc gatcaaaagc aggtgacaag
     4861 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
     4921 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
     4981 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
     5041 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     5101 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     5161 tttcagtcag tcgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     5221 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     5281 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     5341 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     5401 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     5461 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     5521 gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     5581 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     5641 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     5701 cttaatgaat tacaacagta ctgcgatgag tggcaggcgg ggcgtaatct agaggatccg
     5761 gcttactaaa agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa
     5821 tatatactga tatgtatacc cgaagtatgt caaaaagagg tatgctatga agcagcgtat
     5881 tacagtgaca gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc
     5941 tccggtctgg taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg
     6001 aaagcggaaa atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct
     6061 tttgctgacg agaacagggg ctggtgaaat gcagtttaag gtttacacct ataaaagaga
     6121 gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg
     6181 gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtccccc gtgaacttta
     6241 cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt
     6301 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa
     6361 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca
     6421 gtctgcaggt cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
     6481 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag
     6541 ctttcttgta caaagtggtc acctgcaaaa agtgtttgat cgccggcggt acaaagtggt
     6601 taaaataata ttttatttat ctcatgtcat tcgattacag aggctcggct acgagcaaag
     6661 acaaaccaaa tataacaaac aacaaccctt acacaatgac atcggaaaac gaaatacaac
     6721 accctgagat attacattta tagaaactgt acgccgtccg cgctaggaca gtcactgcga
     6781 agcagtgacg tcttcgccgg aggcgaacga gtagttgatg aacgtctcgc cttcatacat
     6841 gtagtgaaca acagtgttag agtacatgta atccgactgt tcgggagtca tatccttgag
     6901 ccaatcttcg tctggattaa ctaaaatgat gcaaggtatt ccaccccgta tgacctttcg
     6961 cttaccatat tttggattga ccgtgaagtc acgctgagcc ccgacgaagc acttccagtt
     7021 gggtgtgaac ttgaatggaa tgtcgtcgat gatattatac ttggcgttga cgtcatatgt
     7081 tgtgaaatca actagactgt tataataatt gtgtgtccct agagaccttg cccaggaagt
     7141 ctttcctgtt ctggttggcc cgcagatgta gatggactta tgcctccccg gtgactcctg
     7201 gaataatcgt ccatccactc taagtcagat tgcgcttgat ccgcaggagt ggaagtacaa
     7261 aggatatagg attcgaggct tacggagtag agatgttcat ttttccagct ttcaatggtc
     7321 tcatggcaaa tgagtgattc ggttggaaac tcaggtgtgt aagtggcaac tgggtcagga
     7381 aatagatggc gtgccgtgta ctcgaagtct ttgagacgga tagaccattc aaacggaaaa
     7441 cgattgcaaa ccatgctgag gaattcctcg cgagaggaac tagattcaat gatctgtttc
     7501 atatccgcat cacggtcttt acgacctgga gttgaaacag ccacgaatgt tccccactca
     7561 gctgtgttta catcggagtc aacctccttc gtgatgtaat cacgaacttg gttgcagtct
     7621 ttggcagctt gtatatttgg atggaatatg gagaatggag atgtatccat acggaggttt
     7681 aaggcattgg gattggtgat ggaagcacga agcttgttct gcacgagaac gtgcagatgt
     7741 ggtgatccat cttcgtggag ctctctaaca gcagcgatgt agaggggctc atatttgttc
     7801 aagagagtgc gaagtgaatc caaggcgtac tgtggctcaa gggtacattg aggatatgtt
     7861 agaaagaggt acttggaata gacacggaac ctgggtgcag atgaagaggc catggtagtg
     7921 aacagaagtc cggcaggtcc ttagcgaaaa aacggggtgt gccagaaaac tctatcctct
     7981 accctgcgtg gaggtgtgaa ttctgcacac tgcaaatgca atgtgtccaa tgctttatat
     8041 agggcaggtt ttggcgggag aacagggccc tagtgttccc acggtagcgt agcgaatcgt
     8101 gtgggccctg ttcggtgtgc ggtcgggggg cctccacgcg ggttataata ttaccccgcg
     8161 tggtggcccc cgacgcgcac tcggcttttc gtgagtgcgc ggaggctttt ggaccacatc
     8221 ttttctgatc actttcgtgg aagatgttga tttatcacac ttttgacggg gaaatctgtg
     8281 ccatgcctta gcttataagg aagtgcgtgg tagcccatct cggggccctc gattcgacgt
     8341 tcctgtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
     8401 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     8461 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     8521 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     8581 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     8641 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     8701 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     8761 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     8821 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     8881 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     8941 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     9001 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     9061 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     9121 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     9181 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     9241 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     9301 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     9361 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     9421 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     9481 cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     9541 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     9601 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     9661 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     9721 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     9781 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     9841 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     9901 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     9961 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
    10021 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
    10081 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
    10141 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
    10201 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
    10261 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
    10321 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
    10381 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
    10441 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
    10501 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
    10561 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
    10621 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
    10681 gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
    10741 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
    10801 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
    10861 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
    10921 cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt
    10981 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
    11041 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
    11101 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
    11161 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
    11221 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
    11281 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
    11341 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
    11401 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
    11461 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
    11521 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
    11581 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
    11641 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
    11701 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
    11761 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
    11821 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggcctct
    11881 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
    11941 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
    12001 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
    12061 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc ccaagagctg caaaaagcgc
    12121 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
    12181 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
    12241 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt
    12301 gatgacggtg aaaacctctg acacatgcag ctcccggaaa cggtcacagc ttgtctgtaa
    12361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    12421 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
    12481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
    12541 taaggagaaa ataccgcatc aggccctctt ccgcttcctc gctcactgac tcgctgcgct
    12601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    13021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    13081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tat
//
