LOCUS       sequence-182154-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XFlag::tagRFP::linker::TEV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Paix A, Folkmann A, Seydoux G
  TITLE     Precision genome editing using CRISPR-Cas9 and linear repair 
            templates in C. elegans.
  JOURNAL   Methods. 2017 May 15;121-122:86-93. doi: 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7.
  PUBMED    28392263
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods."; 
            date: "2017-05-15"; volume: "121-122:86-93. doi"; pages: " 
            10.1016/j.ymeth.2017.03.023. Epub 2017 Apr 7"
COMMENT     Sequence Label: pAP997-1
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             82..789
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLN"
     CDS             841..861
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 cggtacccgg ggatcgacta caaagaccat gacggtgatt ataaagatca tgatatcgat
       61 tacaaggatg acgatgacaa ggtgtctaag ggcgaagagc tgattaagga gaacatgcac
      121 atgaagctgt acatggaggg caccgtgaac aaccaccact tcaaatgcac atccgagggc
      181 gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga gggcggccct
      241 ctccccttcg cctttgacat cctggctacc agcttcatgt acggcagcag aaccttcatc
      301 aaccacaccc agggcatccc cgacttcttt aagcagtcct ttcctgaggg cttcacatgg
      361 gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc
      421 caggacggct gtctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc
      481 cctgtgatgc agaagaaaac actcggctgg gaggccaata ccgagatgct gtaccccgct
      541 gacggcggcc tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg gggccacctg
      601 atctgcaatt tcaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc
      661 ggcgtctact atgtggacca cagactggaa agaatcaaag aggccgacaa agagacctac
      721 gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac
      781 aaacttaatt gcccggggga tcggtggagc tccaccggtg gcggccgctc tagaactagt
      841 gagaacctct acttccaagg agatcctcta gagtcg
//
