LOCUS       sequence-182158-        1627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of hydroxylated Hif1a TAD (776-826).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Sugase K, Landes MA, Wright PE, Martinez-Yamout M
  TITLE     Overexpression of post-translationally modified peptides in 
            Escherichia coli by co-expression with modifying enzymes.
  JOURNAL   Protein Expr Purif. 2008 Feb;57(2):108-15. Epub 2007 Nov 1.
  PUBMED    18054500
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2008-02"; volume: "57(2)"; pages: "108-15. Epub
            2007 Nov 1"
COMMENT     Sequence Label: HisGB1-Hif1a(776-826)_FIH
FEATURES             Location/Qualifiers
     source          1..1627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             14..36
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             82..243
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             268..285
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     RBS             471..493
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     regulatory      498..507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1572..1589
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctctagaat aattttgttt aactttaaga aggagatata ccatgggcca tcatcatcat
       61 catcacagca gcggcatgac ttacaaactg atccttaacg gtaaaacttt gaaaggcgaa
      121 accactaccg aagctgttga tgctgcgact gcagaaaaag ttttcaaaca gtacgctaac
      181 gacaacggtg ttgacggtga atggacttac gacgatgcga ctaagacctt caccgttact
      241 gaagaattca gcagcggcag cagcggcctg gttccgcgtg gttcccatat gtctgattta
      301 gcatgtagac tgctggggca atcaatggat gaaagtggat taccacagct gaccagttat
      361 gattgtgaag ttaatgctcc tatacaaggc agcagaaacc tactgcaggg tgaagaatta
      421 ctcagagctt tggatcaagt taactgatag ggatcccctc tagaaataat tttgtttaac
      481 tttaagaagg agatatacca tggcggcgac agcggcggag gctgtggcct ctggctctgg
      541 agagccccgg gaggaggctg gagccctcgg ccccgcctgg gatgaatccc agttgcgcag
      601 ttatagcttc ccgactaggc ccattccgcg tctgagtcag agcgaccccc gggcagagga
      661 gcttattgag aatgaggagc ctgtggtgct gaccgacaca aatcttgtgt atcctgccct
      721 gaaatgggac cttgaatacc tgcaagagaa tattggcaat ggagacttct ctgtgtacag
      781 tgccagcacc cacaagttct tgtactatga tgagaagaag atggccaatt tccagaactt
      841 taagccgagg tccaacaggg aagaaatgaa atttcatgag ttcgttgaga aactgcagga
      901 tatacagcag cgaggagggg aagagaggtt gtatctgcag caaacgctca atgacactgt
      961 gggcaggaag attgtcatgg acttcttagg ttttaactgg aactggatta ataagcaaca
     1021 gggaaagcgt ggctgggggc agcttacctc taacctgctg ctcattggca tggaaggaaa
     1081 tgtgacacct gctcactatg atgagcagca gaactttttt gctcagataa aaggttacaa
     1141 acgatgcatc ttattccctc cggatcagtt cgagtgcctc tacccatacc ctgttcatca
     1201 cccatgtgac agacagagcc aggtggactt tgacaatccc gactacgaga ggttccctaa
     1261 tttccaaaat gtggttggtt acgaaacagt ggttggccct ggtgatgttc tttacatccc
     1321 aatgtactgg tggcatcaca tagagtcatt actaaatggg gggattacca tcactgtgaa
     1381 cttctggtat aagggggctc ccacccctaa gagaattgaa tatcctctca aagctcatca
     1441 gaaagtggcc ataatgagaa acattgagaa gatgcttgga gaggccttgg ggaacccaca
     1501 agaggtgggg cccttgttga acacaatgat caagggccga tacaactagt aagcttgcgg
     1561 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaaga
     1621 agcgagt
//
