LOCUS       sequence-182169-        4317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal AID*-9myc degron cassette with NatR marker for S. 
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Morawska M, Ulrich HD
  TITLE     An expanded tool kit for the auxin-inducible degron system in 
            budding yeast.
  JOURNAL   Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 
            23.
  PUBMED    23836714
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.2967"; 
            journalName: "Yeast"; date: "2013-09"; volume: "30"; issue: "9"; 
            pages: "341-51"
COMMENT     Sequence Label: pNat-AID*-9myc
FEATURES             Location/Qualifiers
     source          1..4317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             79..210
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             232..261
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             268..297
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             304..333
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             352..381
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             388..417
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             424..453
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             472..501
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             508..537
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             544..573
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        648..991
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             992..1567
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGEAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGERQALYMSMPCP"
     terminator      1575..1762
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1630..1654)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(1954..1973)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1955..1973)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2060..2077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2231..2819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2311..2330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2990..3781,3782..3850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3613..3632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3851..3955)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4023..4041
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4079..4101)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4201..4220
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        join(4301..4317,1..2)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     join(4301..4317,1)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggtgcagg cgccggggct
       61 ggtgctgggg caggtacccc taaagatcca gccaaacctc cggccaaggc acaagttgtg
      121 ggatggccac cggtgagatc ataccggaag aacgtgatgg tttcctgcca aaaatcaagc
      181 ggtggcccgg aggcggcggc gttcgtgaag gtatcacccg gggctagtgg tgaacaaaag
      241 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      301 ggtgaacaaa aattaatctc agaagaagac ttgaacggat cctctagagg tgaacaaaag
      361 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      421 ggtgaacaaa aattaatctc agaagaagac ttgaacggat cctctagagg tgaacaaaag
      481 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac
      541 ggtgaacaaa aattaatctc agaagaagac ttgaacggat ccactagcta aaaaggcgcg
      601 ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc
      661 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc
      721 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc
      781 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc
      841 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt
      901 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca
      961 gttctcacat cacatccgaa cataaacaac catgggtacc actcttgacg acacggctta
     1021 ccggtaccgc accagtgtcc cgggggaagc cgaggccatc gaggcactgg atgggtcctt
     1081 caccaccgac accgtcttcc gcgtcaccgc caccggggac ggcttcaccc tgcgggaggt
     1141 gccggtggac ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg acgaatcgga
     1201 cgacggggag gacggcgacc cggactcccg gacgttcgtc gcgtacgggg acgacggcga
     1261 cctggcgggc ttcgtggtca tctcgtactc ggcgtggaac cgccggctga ccgtcgagga
     1321 catcgaggtc gccccggagc accgggggca cggggtcggg cgcgcgttga tggggctcgc
     1381 gacggagttc gccggcgagc ggggcgccgg gcacctctgg ctggaggtca ccaacgtcaa
     1441 cgcaccggcg atccacgcgt accggcggat ggggttcacc ctctgcggcc tggacaccgc
     1501 cctgtacgac ggcaccgcct cggacggcga gcggcaggcg ctctacatga gcatgccctg
     1561 cccctaatct cgaggcgaat ttcttatgat ttatgatttt tattattaaa taagttataa
     1621 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttgt
     1681 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc
     1741 ttattgacca cacctctacc ggcatgccga gcaaatgcct gcaaatcgct ccccatttca
     1801 cccaattgta gatatgctaa ctccagcaat gagttgatga atctcggtgt gtattttatg
     1861 tcctcagagg acaacacctg ttgtaatcga gctcgaattc atcgatgata tcagatccac
     1921 tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga
     1981 taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2401 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     2461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2641 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     2701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3961 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4021 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     4081 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     4141 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     4201 cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg ttagaacgcg
     4261 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactata
//
